2XKO | pdb_00002xko

Crystal structure of the complex of NtcA with its transcriptional co- activator PipX

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C	SCOP2 Family	PipX-like	8047162	4005478	SCOP2 (2022-06-29)
C	SCOP2 Superfamily	PipX-like	8092203	3002570	SCOP2 (2022-06-29)
D	SCOP2B Superfamily	PipX-like	8092203	3002570	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	DUF3539	e2xkoC1	A: a+b two layers	X: PipX (From Topology)	H: PipX (From Topology)	T: PipX	F: DUF3539	ECOD (1.6)
D	DUF3539	e2xkoD1	A: a+b two layers	X: PipX (From Topology)	H: PipX (From Topology)	T: PipX	F: DUF3539	ECOD (1.6)
A	cNMP_binding	e2xkoA1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
A	HTH_Crp_2	e2xkoA2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)
B	cNMP_binding	e2xkoB1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
B	HTH_Crp_2	e2xkoB2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C	2.30.30.660	Mainly Beta	Roll	SH3 type barrels.	Protein of unknown function (DUF3539)	CATH (4.3.0)
C	6.10.250.870	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
D	2.30.30.660	Mainly Beta	Roll	SH3 type barrels.	Protein of unknown function (DUF3539)	CATH (4.3.0)
D	6.10.250.870	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
A	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
B	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
B	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF13545	Crp-like helix-turn-helix domain (HTH_Crp_2)	Crp-like helix-turn-helix domain	This family represents a crp-like helix-turn-helix domain that is likely to bind DNA.	Domain
A, B	PF00027	Cyclic nucleotide-binding domain (cNMP_binding)	Cyclic nucleotide-binding domain	This domain sensor domain can bind cAMP, cGMP, c-di-GMP, oxygen and 2-oxoglutarate (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
C, D	PIPX	-	-	-
A, B	GLOBAL NITROGEN REGULATOR	DNA binding binding nucleic acid binding transcription regulator activity DNA-binding transcription factor activity	-	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
C, D	IPR021926	PII-interacting protein X, cyanobacteria	Family
A, B	IPR050397	Global Transcriptional Regulators in Environmental Response	Family
A, B	IPR000595	Cyclic nucleotide-binding domain	Domain
A, B	IPR018335	Transcription regulator HTH, Crp-type, conserved site	Conserved Site
A, B	IPR018490	Cyclic nucleotide-binding domain superfamily	Homologous Superfamily
A, B	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, B	IPR022299	Transcription regulator, NtcA	Family
A, B	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
A, B	IPR012318	Crp-type HTH domain	Domain
A, B	IPR014710	RmlC-like jelly roll fold	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.