8BEF

Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CI membrane core)

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
D [auth K]	SCOP2B Superfamily	NADH-quinone oxidoreductase, subunit 11-like	8101595	3002200	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
K [auth Z]	GRIM-19	e8befZ1	A: extended segments	X: Mitochondrial complex I, B16.6 subunit (From Topology)	H: Mitochondrial complex I, B16.6 subunit (From Topology)	T: Mitochondrial complex I, B16.6 subunit	F: GRIM-19	ECOD (1.6)
L [auth a]	MWFE	e8befa1	A: extended segments	X: Mitochondrial complex I, MWFE subunit (From Topology)	H: Mitochondrial complex I, MWFE subunit (From Topology)	T: Mitochondrial complex I, MWFE subunit	F: MWFE	ECOD (1.6)
O [auth e]	Ndufs5	e8befe1	A: alpha bundles	X: cytochrome bc1 complex 11 kDa protein-like	H: CHCH domain (From Topology)	T: CHCH domain	F: Ndufs5	ECOD (1.6)
B [auth H]	NADHdh	e8befH1	A: alpha complex topology	X: Sodium/proton antiporter subunits-like (From Homology)	H: Sodium/proton antiporter subunits-like	T: NADH-quinone oxidoreductase subunit 8 (Nqo8)-related	F: NADHdh	ECOD (1.6)
T [auth x]	Hexapep_2_1	e8befx1	A: beta duplicates or obligate multimers	X: Single-stranded left-handed beta-helix	H: Trimeric LpxA-like enzymes (From Topology)	T: Trimeric LpxA-like enzymes	F: Hexapep_2_1	ECOD (1.6)
D [auth K]	Oxidored_q2	e8befK1	A: alpha complex topology	X: NADH-quinone oxidoreductase subunit K (From Topology)	H: NADH-quinone oxidoreductase subunit K (From Topology)	T: NADH-quinone oxidoreductase subunit K	F: Oxidored_q2	ECOD (1.6)
G [auth N]	Proton_antipo_M	e8befN1	A: alpha complex topology	X: Sodium/proton antiporter subunits-like (From Homology)	H: Sodium/proton antiporter subunits-like	T: NADH-quinone oxidoreductase subunit L (NuoL)-related	F: Proton_antipo_M	ECOD (1.6)
H [auth O]	Fer2	e8befO1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: Fer2	ECOD (1.6)
I [auth X]	F_UNCLASSIFIED	e8befX1	A: few secondary structure elements	X: p8-MTCP1-related (From Topology)	H: p8-MTCP1-related (From Topology)	T: p8-MTCP1-related	F: F_UNCLASSIFIED	ECOD (1.6)
I [auth X]	CHCH	e8befX2	A: few secondary structure elements	X: p8-MTCP1-related (From Topology)	H: p8-MTCP1-related (From Topology)	T: p8-MTCP1-related	F: CHCH	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
U [auth y]	2.160.10.10	Mainly Beta	3 Solenoid	UDP N-Acetylglucosamine Acyltransferase	domain 1	CATH (utative)
V [auth z]	2.160.10.10	Mainly Beta	3 Solenoid	UDP N-Acetylglucosamine Acyltransferase	domain 1	CATH (utative)
C [auth J]	1.20.120.1200	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
F [auth M]	PF00361	Proton-conducting membrane transporter (Proton_antipo_M)	Proton-conducting membrane transporter	-	Family
J [auth Y]	PF00132	Bacterial transferase hexapeptide (six repeats) (Hexapep)	Bacterial transferase hexapeptide (six repeats)	-	Repeat
J [auth Y]	PF02466	Tim17/Tim22/Tim23/Pmp24 family (Tim17)	Tim17/Tim22/Tim23/Pmp24 family	-	Family
A	PF15879	NADH-ubiquinone oxidoreductase MWFE subunit (MWFE)	NADH-ubiquinone oxidoreductase MWFE subunit	-	Family
A	PF00507	NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 (Oxidored_q4)	NADH-ubiquinone/plastoquinone oxidoreductase, chain 3	-	Family
B [auth H]	PF00146	NADH dehydrogenase (NADHdh)	NADH dehydrogenase	-	Family
C [auth J]	PF00499	NADH-ubiquinone/plastoquinone oxidoreductase chain 6 (Oxidored_q3)	NADH-ubiquinone/plastoquinone oxidoreductase chain 6	-	Family
D [auth K]	PF00420	NADH-ubiquinone/plastoquinone oxidoreductase chain 4L (Oxidored_q2)	NADH-ubiquinone/plastoquinone oxidoreductase chain 4L	-	Family
G [auth N]	PF00361	Proton-conducting membrane transporter (Proton_antipo_M)	Proton-conducting membrane transporter	-	Family
I [auth X]	PF06747	CHCH domain (CHCH)	CHCH domain	we have identified a conserved motif in the LOC118487 protein that we have called the CHCH motif. Alignment of this protein with related members showed the presence of three subgroups of proteins, which are called the S (Small), N (N-terminal extende ...	we have identified a conserved motif in the LOC118487 protein that we have called the CHCH motif. Alignment of this protein with related members showed the presence of three subgroups of proteins, which are called the S (Small), N (N-terminal extended) and C (C-terminal extended) subgroups. All three sub-groups of proteins have in common that they contain a predicted conserved [coiled coil 1]-[helix 1]-[coiled coil 2]-[helix 2] domain (CHCH domain). Within each helix of the CHCH domain, there are two cysteines present in a C-X9-C motif. The N-group contains an additional double helix domain, and each helix contains the C-X9-C motif. This family contains a number of characterised proteins: Cox19 protein - a nuclear gene of Saccharomyces cerevisiae, codes for an 11-kDa protein (Cox19p) required for expression of cytochrome oxidase. Because cox19 mutants are able to synthesise the mitochondrial and nuclear gene products of cytochrome oxidase, Cox19p probably functions post-translationally during assembly of the enzyme. Cox19p is present in the cytoplasm and mitochondria, where it exists as a soluble intermembrane protein. This dual location is similar to what was previously reported for Cox17p, a low molecular weight copper protein thought to be required for maturation of the CuA centre of subunit 2 of cytochrome oxidase. Cox19p have four conserved potential metal ligands, these are three cysteines and one histidine. Mrp10 - belongs to the class of yeast mitochondrial ribosomal proteins that are essential for translation [2]. Eukaryotic NADH-ubiquinone oxidoreductase 19 kDa (NDUFA8) subunit [3]. The CHCH domain was previously called DUF657 [4].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
E [auth L]	NADH-ubiquinone oxidoreductase chain 5	oxidoreductase activity oxidoreductase activity, acting on NAD(P)H catalytic activity NADH dehydrogenase activity monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity oxidoreductase activity oxidoreductase activity, acting on NAD(P)H catalytic activity NADH dehydrogenase activity monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity	aerobic respiration cellular respiration oxidative phosphorylation energy derivation by oxidation of organic compounds respiratory electron transport chain ATP synthesis coupled electron transport electron transport chain metabolic process cellular process generation of precursor metabolites and energy electron transport coupled proton transport transport proton transmembrane transport monoatomic ion transmembrane transport aerobic respiration cellular respiration oxidative phosphorylation energy derivation by oxidation of organic compounds respiratory electron transport chain ATP synthesis coupled electron transport electron transport chain metabolic process cellular process generation of precursor metabolites and energy electron transport coupled proton transport transport proton transmembrane transport monoatomic ion transmembrane transport inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization energy coupled proton transmembrane transport, against electrochemical gradient monoatomic ion transport monoatomic cation transport	membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm organelle membrane respiratory chain complex protein-containing complex catalytic complex membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex plastid
F [auth M]	NADH-ubiquinone oxidoreductase chain 4	binding quinone binding ubiquinone binding small molecule binding monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity binding quinone binding ubiquinone binding small molecule binding monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity catalytic activity	aerobic respiration cellular respiration oxidative phosphorylation energy derivation by oxidation of organic compounds respiratory electron transport chain ATP synthesis coupled electron transport electron transport chain metabolic process cellular process generation of precursor metabolites and energy electron transport coupled proton transport transport proton transmembrane transport monoatomic ion transmembrane transport aerobic respiration cellular respiration oxidative phosphorylation energy derivation by oxidation of organic compounds respiratory electron transport chain ATP synthesis coupled electron transport electron transport chain metabolic process cellular process generation of precursor metabolites and energy electron transport coupled proton transport transport proton transmembrane transport monoatomic ion transmembrane transport inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization energy coupled proton transmembrane transport, against electrochemical gradient monoatomic ion transport monoatomic cation transport	membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm organelle membrane respiratory chain complex protein-containing complex catalytic complex membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex plastid
J [auth Y]	Outer envelope pore protein 16-3, chloroplastic/mitochondrial	wide pore channel activity passive transmembrane transporter activity channel activity porin activity transporter activity transmembrane transporter activity identical protein binding protein binding binding protein homodimerization activity protein dimerization activity	protein localization establishment of protein localization cellular localization nitrogen compound transport transport establishment of protein localization to mitochondrial membrane protein targeting mitochondrial membrane organization cellular component organization localization cellular macromolecule localization establishment of localization in cell cellular component organization or biogenesis protein insertion into mitochondrial inner membrane protein localization establishment of protein localization cellular localization nitrogen compound transport transport establishment of protein localization to mitochondrial membrane protein targeting mitochondrial membrane organization cellular component organization localization cellular macromolecule localization establishment of localization in cell cellular component organization or biogenesis protein insertion into mitochondrial inner membrane mitochondrial transport mitochondrion organization membrane organization establishment of protein localization to membrane inner mitochondrial membrane organization intracellular transport localization within membrane protein localization to membrane establishment of localization organelle organization establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle protein localization to mitochondrion protein targeting to mitochondrion cellular process establishment of protein localization to mitochondrion intracellular protein transport monoatomic ion transport	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle plastid membrane plastid membrane chloroplast envelope organelle outer membrane plastid outer membrane membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle plastid membrane plastid membrane chloroplast envelope organelle outer membrane plastid outer membrane chloroplast outer membrane organelle envelope bounding membrane of organelle chloroplast membrane outer membrane organelle membrane plastid envelope chloroplast mitochondrial protein-containing complex protein-containing complex mitochondrial membrane organelle inner membrane membrane protein complex TIM22 mitochondrial import inner membrane insertion complex mitochondrial inner membrane inner mitochondrial membrane protein complex mitochondrial envelope cytosol mitochondrial outer membrane pore complex
M [auth b]	At2g46540/F11C10.23	-	-	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle mitochondrion organelle intracellular membrane-bounded organelle
S [auth v]	Uncharacterized protein At2g27730, mitochondrial	cation binding transition metal ion binding copper ion binding ion binding binding metal ion binding small molecule binding	metabolic process cellular process cellular metabolic compound salvage photorespiration	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle respiratory chain complex protein-containing complex catalytic complex membrane membrane protein complex transporter complex membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle respiratory chain complex protein-containing complex catalytic complex membrane membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex nucleus membrane-enclosed lumen intracellular membraneless organelle nucleolus intracellular organelle lumen organelle lumen membraneless organelle nuclear lumen plant-type vacuole vacuole peroxisome microbody mitochondrial membrane organelle envelope mitochondrial envelope organelle membrane
A	NADH-ubiquinone oxidoreductase chain 3	monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity catalytic activity	-	protein-containing complex cellular anatomical structure catalytic complex membrane membrane protein complex oxidoreductase complex NADH dehydrogenase complex membrane-bounded organelle mitochondrial membrane intracellular anatomical structure mitochondrion intracellular membrane-bounded organelle intracellular organelle organelle envelope protein-containing complex cellular anatomical structure catalytic complex membrane membrane protein complex oxidoreductase complex NADH dehydrogenase complex membrane-bounded organelle mitochondrial membrane intracellular anatomical structure mitochondrion intracellular membrane-bounded organelle intracellular organelle organelle envelope mitochondrial envelope organelle cytoplasm organelle membrane
K [auth Z]	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A	-	metabolic process cellular process cellular metabolic compound salvage photorespiration developmental process multicellular organismal process reproductive system development reproductive process embryo development developmental process involved in reproduction multicellular organism development fruit development embryo development ending in seed dormancy seed development metabolic process cellular process cellular metabolic compound salvage photorespiration developmental process multicellular organismal process reproductive system development reproductive process embryo development developmental process involved in reproduction multicellular organism development fruit development embryo development ending in seed dormancy seed development anatomical structure development reproductive structure development post-embryonic development system development	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane organelle inner membrane membrane mitochondrial inner membrane organelle envelope mitochondrial envelope membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane organelle inner membrane membrane mitochondrial inner membrane organelle envelope mitochondrial envelope organelle membrane respiratory chain complex protein-containing complex catalytic complex membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex extracellular region
L [auth a]	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1	-	-	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane organelle inner membrane membrane mitochondrial inner membrane organelle envelope mitochondrial envelope membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane organelle inner membrane membrane mitochondrial inner membrane organelle envelope mitochondrial envelope organelle membrane
N [auth d]	Excitatory amino acid transporter	-	-	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle mitochondrion organelle intracellular membrane-bounded organelle plant-type vacuole vacuole
O [auth e]	NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B	-	-	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle mitochondrion organelle intracellular membrane-bounded organelle mitochondrial membrane organelle inner membrane membrane mitochondrial inner membrane organelle envelope cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle mitochondrion organelle intracellular membrane-bounded organelle mitochondrial membrane organelle inner membrane membrane mitochondrial inner membrane organelle envelope mitochondrial envelope organelle membrane mitochondrial intermembrane space membrane-enclosed lumen intracellular organelle lumen organelle envelope lumen organelle lumen
P [auth f]	At4g16450	-	metabolic process cellular process cellular metabolic compound salvage photorespiration	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle mitochondrion organelle intracellular membrane-bounded organelle respiratory chain complex protein-containing complex catalytic complex membrane membrane protein complex cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle mitochondrion organelle intracellular membrane-bounded organelle respiratory chain complex protein-containing complex catalytic complex membrane membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex mitochondrial membrane organelle envelope mitochondrial envelope organelle membrane
Q [auth i]	P1	-	metabolic process cellular process cellular metabolic compound salvage photorespiration	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle respiratory chain complex protein-containing complex catalytic complex membrane membrane protein complex transporter complex membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle respiratory chain complex protein-containing complex catalytic complex membrane membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex mitochondrial membrane organelle envelope mitochondrial envelope organelle membrane
R [auth u]	Uncharacterized protein At1g67785	-	-	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle
B [auth H]	NADH-ubiquinone oxidoreductase chain 1	monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity catalytic activity	aerobic respiration cellular respiration energy derivation by oxidation of organic compounds metabolic process cellular process generation of precursor metabolites and energy	membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm organelle membrane respiratory chain complex protein-containing complex catalytic complex membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex
T [auth x]	Gamma carbonic anhydrase-like 2, mitochondrial	cation binding ion binding binding metal ion binding small molecule binding	metabolic process cellular process cellular metabolic compound salvage photorespiration response to abscisic acid response to stimulus response to lipid response to hormone response to alcohol response to chemical response to endogenous stimulus response to oxygen-containing compound developmental process multicellular organismal process metabolic process cellular process cellular metabolic compound salvage photorespiration response to abscisic acid response to stimulus response to lipid response to hormone response to alcohol response to chemical response to endogenous stimulus response to oxygen-containing compound developmental process multicellular organismal process reproductive system development reproductive process vegetative to reproductive phase transition of meristem developmental process involved in reproduction multicellular organism development anatomical structure development reproductive structure development post-embryonic development system development response to osmotic stress response to stress response to abiotic stimulus response to salt stress	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane membrane organelle envelope mitochondrial envelope organelle membrane cytosol membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane membrane organelle envelope mitochondrial envelope organelle membrane cytosol respiratory chain complex protein-containing complex catalytic complex membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex plant-type vacuole vacuole
U [auth y]	Gamma carbonic anhydrase 2, mitochondrial	carbonate dehydratase activity hydro-lyase activity lyase activity catalytic activity carbon-oxygen lyase activity cation binding ion binding binding metal ion binding small molecule binding identical protein binding protein binding	metabolic process cellular process cellular metabolic compound salvage photorespiration multicellular organismal process reproductive process developmental process involved in reproduction reproductive shoot system development multicellular organism development anther development anatomical structure development reproductive structure development post-embryonic development phyllome development metabolic process cellular process cellular metabolic compound salvage photorespiration multicellular organismal process reproductive process developmental process involved in reproduction reproductive shoot system development multicellular organism development anther development anatomical structure development reproductive structure development post-embryonic development phyllome development androecium development system development developmental process plant organ development floral whorl development reproductive system development dehiscence stamen development multicellular organismal reproductive process flower development shoot system development anther dehiscence floral organ development protein-containing complex organization protein trimerization cellular component organization cellular component biogenesis cellular component assembly protein homotrimerization protein complex oligomerization protein-containing complex assembly protein homooligomerization cellular component organization or biogenesis regulation of biological process regulation of cellular process reactive oxygen species metabolic process biological regulation regulation of metabolic process regulation of reactive oxygen species metabolic process	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane membrane organelle envelope mitochondrial envelope organelle membrane respiratory chain complex membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle mitochondrial membrane membrane organelle envelope mitochondrial envelope organelle membrane respiratory chain complex protein-containing complex catalytic complex membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex nucleus
V [auth z]	Gamma carbonic anhydrase 1, mitochondrial	carbonate dehydratase activity hydro-lyase activity lyase activity catalytic activity carbon-oxygen lyase activity cation binding ion binding binding metal ion binding small molecule binding	metabolic process cellular process cellular metabolic compound salvage photorespiration protein-containing complex organization protein trimerization cellular component organization cellular component biogenesis cellular component assembly protein homotrimerization protein complex oligomerization protein-containing complex assembly protein homooligomerization cellular component organization or biogenesis	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle respiratory chain complex protein-containing complex catalytic complex membrane membrane protein complex transporter complex membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle respiratory chain complex protein-containing complex catalytic complex membrane membrane protein complex transporter complex NADH dehydrogenase complex transmembrane transporter complex respiratory chain complex I oxidoreductase complex nucleus mitochondrial membrane organelle envelope mitochondrial envelope organelle membrane
C [auth J]	NADH-ubiquinone oxidoreductase chain 6	monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity catalytic activity	-	membrane-bounded organelle mitochondrial membrane intracellular anatomical structure mitochondrion membrane intracellular membrane-bounded organelle intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm organelle membrane
D [auth K]	NADH-ubiquinone oxidoreductase chain 4L	monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity catalytic activity	aerobic respiration cellular respiration oxidative phosphorylation energy derivation by oxidation of organic compounds respiratory electron transport chain ATP synthesis coupled electron transport electron transport chain metabolic process cellular process generation of precursor metabolites and energy	protein-containing complex cellular anatomical structure catalytic complex membrane membrane protein complex oxidoreductase complex NADH dehydrogenase complex membrane-bounded organelle mitochondrial membrane intracellular anatomical structure mitochondrion intracellular membrane-bounded organelle intracellular organelle organelle envelope protein-containing complex cellular anatomical structure catalytic complex membrane membrane protein complex oxidoreductase complex NADH dehydrogenase complex membrane-bounded organelle mitochondrial membrane intracellular anatomical structure mitochondrion intracellular membrane-bounded organelle intracellular organelle organelle envelope mitochondrial envelope organelle cytoplasm organelle membrane
G [auth N]	NADH-ubiquinone oxidoreductase chain 2	monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity monoatomic cation transmembrane transporter activity proton transmembrane transporter activity primary active transmembrane transporter activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor electron transfer activity active monoatomic ion transmembrane transporter activity oxidoreductase activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity inorganic molecular entity transmembrane transporter activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity catalytic activity	aerobic respiration cellular respiration oxidative phosphorylation energy derivation by oxidation of organic compounds respiratory electron transport chain ATP synthesis coupled electron transport electron transport chain metabolic process cellular process generation of precursor metabolites and energy	membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope cellular anatomical structure mitochondrial envelope organelle cytoplasm organelle membrane plastid
H [auth O]	AT3G07480.1	iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding binding metal cluster binding small molecule binding	P450-containing electron transport chain electron transport chain metabolic process cellular process generation of precursor metabolites and energy transport proton transmembrane transport monoatomic ion transmembrane transport inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization P450-containing electron transport chain electron transport chain metabolic process cellular process generation of precursor metabolites and energy transport proton transmembrane transport monoatomic ion transmembrane transport inorganic ion transmembrane transport establishment of localization inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization monoatomic ion transport monoatomic cation transport aerobic respiration cellular respiration aerobic electron transport chain oxidative phosphorylation energy derivation by oxidation of organic compounds ATP synthesis coupled electron transport mitochondrial electron transport, NADH to ubiquinone respiratory electron transport chain mitochondrial ATP synthesis coupled electron transport	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle mitochondrion organelle intracellular membrane-bounded organelle
I [auth X]	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B	-	aerobic respiration cellular respiration aerobic electron transport chain oxidative phosphorylation energy derivation by oxidation of organic compounds ATP synthesis coupled electron transport electron transport chain generation of precursor metabolites and energy mitochondrial electron transport, NADH to ubiquinone respiratory electron transport chain metabolic process cellular process mitochondrial ATP synthesis coupled electron transport	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle plastid mitochondrial intermembrane space membrane-enclosed lumen intracellular organelle lumen organelle envelope lumen organelle envelope membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle plastid mitochondrial intermembrane space membrane-enclosed lumen intracellular organelle lumen organelle envelope lumen organelle envelope organelle lumen mitochondrial envelope

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
E [auth L]	IPR003945	NADH-quinone oxidoreductase, chain 5-like	Family
E [auth L]	IPR010934	NADH dehydrogenase subunit 5, C-terminal	Domain
E [auth L]	IPR001516	NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal	Domain
E [auth L]	IPR001750	NADH:quinone oxidoreductase/Mrp antiporter, transmembrane domain	Domain
E [auth L]	IPR018393	NADH-plastoquinone oxidoreductase, chain 5 subgroup	Family
F [auth M]	IPR003918	NADH:ubiquinone oxidoreductase	Family
F [auth M]	IPR010227	NADH-quinone oxidoreductase, chain M/4	Family
F [auth M]	IPR001750	NADH:quinone oxidoreductase/Mrp antiporter, transmembrane domain	Domain
J [auth Y]	IPR039175	Mitochondrial import inner membrane translocase subunit TIM22	Family
S [auth v]	IPR045284	At2g27730-like	Family
A	IPR023043	NAD(P)H-quinone oxidoreductase subunit 3, bacterial/plastid	Family
A	IPR000440	NADH:ubiquinone/plastoquinone oxidoreductase, chain 3	Family
A	IPR038430	NADH:ubiquinone oxidoreductase, subunit 3 superfamily	Homologous Superfamily
K [auth Z]	IPR009346	GRIM-19	Family
L [auth a]	IPR017384	NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1	Family
O [auth e]	IPR019342	NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5	Family
P [auth f]	IPR019721	NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal	Domain
P [auth f]	IPR053229	NADH-ubiquinone oxidoreductase subunit	Family
Q [auth i]	IPR035204	NADH-ubiquinone oxidoreductase 11kDa subunit	Family
B [auth H]	IPR001694	NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H	Family
B [auth H]	IPR018086	NADH:ubiquinone oxidoreductase, subunit 1, conserved site	Conserved Site
T [auth x]	IPR047324	Gamma carbonic anhydrase-like	Family
T [auth x]	IPR011004	Trimeric LpxA-like superfamily	Homologous Superfamily
T [auth x]	IPR050484	Transferase Hexapeptide/Carbonic Anhydrase	Family
U [auth y]	IPR047324	Gamma carbonic anhydrase-like	Family
U [auth y]	IPR001451	Hexapeptide repeat	Repeat
U [auth y]	IPR011004	Trimeric LpxA-like superfamily	Homologous Superfamily
U [auth y]	IPR050484	Transferase Hexapeptide/Carbonic Anhydrase	Family
V [auth z]	IPR047324	Gamma carbonic anhydrase-like	Family
V [auth z]	IPR011004	Trimeric LpxA-like superfamily	Homologous Superfamily
V [auth z]	IPR050484	Transferase Hexapeptide/Carbonic Anhydrase	Family
C [auth J]	IPR042106	NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ	Homologous Superfamily
C [auth J]	IPR001457	NADH:ubiquinone/plastoquinone oxidoreductase, chain 6	Family
D [auth K]	IPR001133	NADH-ubiquinone oxidoreductase chain 4L/K	Family
D [auth K]	IPR039428	NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like	Family
G [auth N]	IPR010096	NAD(P)H-quinone oxidoreductase, subunit N/subunit 2	Family
G [auth N]	IPR001750	NADH:quinone oxidoreductase/Mrp antiporter, transmembrane domain	Domain
H [auth O]	IPR001055	Adrenodoxin-like	Family
H [auth O]	IPR036010	2Fe-2S ferredoxin-like superfamily	Homologous Superfamily
H [auth O]	IPR012675	Beta-grasp domain superfamily	Homologous Superfamily
I [auth X]	IPR010625	CHCH	Domain
I [auth X]	IPR016680	NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B [auth H], C [auth J], D [auth K], E [auth L] A, B [auth H], C [auth J], D [auth K], E [auth L], F [auth M], G [auth N], J [auth Y], K [auth Z], L [auth a], O [auth e], R [auth u], S [auth v], T [auth x], U [auth y], V [auth z]	OPM	Transmembrane	Alpha-helical polytopic	Electron transport chain complex I (NADH dehydrogenase) and supercomplexes	H+ or Na+ translocating NADH dehydrogenase

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B [auth H], C [auth J], D [auth K], E [auth L] A, B [auth H], C [auth J], D [auth K], E [auth L], F [auth M], G [auth N], J [auth Y], K [auth Z], L [auth a], O [auth e], R [auth u], S [auth v], T [auth x], U [auth y], V [auth z]	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B [auth H], C [auth J], D [auth K], E [auth L] A, B [auth H], C [auth J], D [auth K], E [auth L], F [auth M], G [auth N], J [auth Y], K [auth Z], L [auth a], O [auth e], R [auth u], S [auth v], T [auth x], U [auth y], V [auth z]	MemProtMD	Annotated as membrane protein by MemProtMD

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A	FME	Parent Component: MET RESID: AA0021 , AA0566 PSI-MOD : N-formyl-L-methionine residue MOD:00030 , N-formyl-L-methionine (Met) MOD:00482 , N-[(L-histidin-1'-yl)methyl]-L-methionine (fMet) MOD:01890 , N-[(L-histidin-1'-yl)methyl]-L-methionine (Met) MOD:01891
P [auth f]	FME	Parent Component: MET RESID: AA0021 , AA0566 , AA0021 , AA0566 PSI-MOD : N-formyl-L-methionine residue MOD:00030 , N-formyl-L-methionine (Met) MOD:00482 , N-[(L-histidin-1'-yl)methyl]-L-methionine (fMet) MOD:01890 , N-[(L-histidin-1'-yl)methyl]-L-methionine (Met) MOD:01891 , N-formyl-L-methionine residue MOD:00030 , N-formyl-L-methionine (Met) MOD:00482 , N-[(L-histidin-1'-yl)methyl]-L-methionine (fMet) MOD:01890 , N-[(L-histidin-1'-yl)methyl]-L-methionine (Met) MOD:01891
D [auth K]	FME	Parent Component: MET RESID: AA0021 , AA0566 , AA0021 , AA0566 , AA0021 , AA0566 PSI-MOD : N-formyl-L-methionine residue MOD:00030 , N-formyl-L-methionine (Met) MOD:00482 , N-[(L-histidin-1'-yl)methyl]-L-methionine (fMet) MOD:01890 , N-[(L-histidin-1'-yl)methyl]-L-methionine (Met) MOD:01891 , N-formyl-L-methionine residue MOD:00030 , N-formyl-L-methionine (Met) MOD:00482 , N-[(L-histidin-1'-yl)methyl]-L-methionine (fMet) MOD:01890 , N-[(L-histidin-1'-yl)methyl]-L-methionine (Met) MOD:01891 , N-formyl-L-methionine residue MOD:00030 , N-formyl-L-methionine (Met) MOD:00482 , N-[(L-histidin-1'-yl)methyl]-L-methionine (fMet) MOD:01890 , N-[(L-histidin-1'-yl)methyl]-L-methionine (Met) MOD:01891