9DZV

Cryo-EM structure of the C. neoformans lipid flippase Apt1-Cdc50 in the E1 state

External Resource: Annotation

Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: PDBTM

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B]	PF03381	LEM3 (ligand-effect modulator 3) family / CDC50 family (CDC50)	LEM3 (ligand-effect modulator 3) family / CDC50 family	-	Family
B [auth A]	PF16209	Phospholipid-translocating ATPase N-terminal (PhoLip_ATPase_N)	Phospholipid-translocating ATPase N-terminal	-	Family
B [auth A]	PF16212	Phospholipid-translocating P-type ATPase C-terminal (PhoLip_ATPase_C)	Phospholipid-translocating P-type ATPase C-terminal	-	Family
B [auth A]	PF13246	Cation transport ATPase (P-type) (Cation_ATPase)	Cation transport ATPase (P-type)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B]	Transcription regulator	-	membrane organization phospholipid translocation transport biological regulation lipid localization phospholipid transport lipid transport lipid translocation establishment of localization regulation of membrane lipid distribution cellular component organization localization macromolecule localization cellular process membrane organization phospholipid translocation transport biological regulation lipid localization phospholipid transport lipid transport lipid translocation establishment of localization regulation of membrane lipid distribution cellular component organization localization macromolecule localization cellular process cellular component organization or biogenesis regulation of biological quality organophosphate ester transport	cell periphery cellular anatomical structure plasma membrane membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure Golgi apparatus organelle cytoplasm intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum
B [auth A]	Phospholipid-transporting ATPase	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding glycerophospholipid flippase activity lipid transporter activity ATPase-coupled intramembrane lipid transporter activity flippase activity intramembrane lipid transporter activity transporter activity phosphatidylethanolamine flippase activity phospholipid transporter activity cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding glycerophospholipid flippase activity lipid transporter activity ATPase-coupled intramembrane lipid transporter activity flippase activity intramembrane lipid transporter activity transporter activity phosphatidylethanolamine flippase activity phospholipid transporter activity ATP-dependent activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	membrane organization phospholipid translocation transport biological regulation lipid localization phospholipid transport lipid transport lipid translocation establishment of localization regulation of membrane lipid distribution cellular component organization localization macromolecule localization cellular process membrane organization phospholipid translocation transport biological regulation lipid localization phospholipid transport lipid transport lipid translocation establishment of localization regulation of membrane lipid distribution cellular component organization localization macromolecule localization cellular process cellular component organization or biogenesis regulation of biological quality organophosphate ester transport	cell periphery cellular anatomical structure plasma membrane membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B]	IPR005045	CDC50/LEM3 family	Family
B [auth A]	IPR023214	HAD superfamily	Homologous Superfamily
B [auth A]	IPR008250	P-type ATPase, A domain superfamily	Homologous Superfamily
B [auth A]	IPR001757	P-type ATPase	Family
B [auth A]	IPR044492	P-type ATPase, haloacid dehalogenase domain	Domain
B [auth A]	IPR006539	P-type ATPase, subfamily IV	Family
B [auth A]	IPR018303	P-type ATPase, phosphorylation site	PTM
B [auth A]	IPR036412	HAD-like superfamily	Homologous Superfamily
B [auth A]	IPR023298	P-type ATPase, transmembrane domain superfamily	Homologous Superfamily
B [auth A]	IPR032631	P-type ATPase, N-terminal	Domain
B [auth A]	IPR032630	P-type ATPase, C-terminal	Domain
B [auth A]	IPR023299	P-type ATPase, cytoplasmic domain N	Homologous Superfamily

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A [auth B], B [auth A]	PDBTM	Annotated as membrane protein by PDBTM

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.