1U0H

STRUCTURAL BASIS FOR THE INHIBITION OF MAMMALIAN ADENYLYL CYCLASE BY MANT-GTP

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d1u0hb_	Alpha and beta proteins (a+b)	Ferredoxin-like	Nucleotide cyclase	Adenylyl and guanylyl cyclase catalytic domain	Type II adenylyl cyclase C2 domain	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
C	d1u0hc1	All alpha proteins	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
C	d1u0hc2	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Transducin (alpha subunit)	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
A	d1u0ha_	Alpha and beta proteins (a+b)	Ferredoxin-like	Nucleotide cyclase	Adenylyl and guanylyl cyclase catalytic domain	Adenylyl cyclase VC1, domain C1a	dog (Canis lupus familiaris ) [TaxId: 9615 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	Nucleotide cyclase/DNA polymerase palm domain-like	8035576	3001272	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Galpha insert domain-like	8056792	3000669	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Ras-like P-loop GTPases	8056791	3002022	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Ras-like P-loop GTPases	8056791	3002022	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Nucleotide cyclase/DNA polymerase palm domain-like	8035568	3001272	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	Guanylate_cyc	e1u0hB1	A: a+b two layers	X: Alpha-beta plaits	H: Adenylyl and guanylyl cyclase catalytic domain-like	T: Adenylyl and guanylyl cyclase catalytic domain-like	F: Guanylate_cyc	ECOD (1.6)
C	G-alpha	e1u0hC1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
C	Ras	e1u0hC2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
A	Guanylate_cyc	e1u0hA1	A: a+b two layers	X: Alpha-beta plaits	H: Adenylyl and guanylyl cyclase catalytic domain-like	T: Adenylyl and guanylyl cyclase catalytic domain-like	F: Guanylate_cyc	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.30.70.1230	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits	Nucleotide cyclase, GGDEF domain	CATH (4.3.0)
C	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
A	3.30.70.1230	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits	Nucleotide cyclase, GGDEF domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00211	Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc)	Adenylate and Guanylate cyclase catalytic domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B	Adenylate cyclase, type II	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding phosphorus-oxygen lyase activity cyclase activity lyase activity adenylate cyclase activity catalytic activity protein binding enzyme binding adenylate cyclase binding manganese ion binding transition metal ion binding protein-containing complex binding G-protein beta/gamma-subunit complex binding	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation adenylate cyclase-activating G protein-coupled receptor signaling pathway cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway cellular process signal transduction ribose phosphate metabolic process nucleotide biosynthetic process cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation adenylate cyclase-activating G protein-coupled receptor signaling pathway cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway cellular process signal transduction ribose phosphate metabolic process nucleotide biosynthetic process carbohydrate derivative metabolic process purine ribonucleotide biosynthetic process cAMP biosynthetic process cyclic nucleotide metabolic process nucleobase-containing compound metabolic process ribonucleotide biosynthetic process purine nucleotide metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine-containing compound biosynthetic process cyclic nucleotide biosynthetic process phosphate-containing compound metabolic process small molecule metabolic process phosphorus metabolic process ribose phosphate biosynthetic process nucleoside phosphate metabolic process carbohydrate derivative biosynthetic process cAMP metabolic process cyclic purine nucleotide metabolic process primary metabolic process nucleotide metabolic process nucleobase-containing compound biosynthetic process purine-containing compound metabolic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process nucleoside phosphate biosynthetic process metabolic process purine ribonucleotide metabolic process purine nucleotide biosynthetic process adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway intracellular signal transduction cAMP-mediated signaling intracellular signaling cassette response to ketone response to lipid response to alcohol cellular response to forskolin cellular response to organic cyclic compound response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to alcohol response to chemical response to forskolin cellular response to lipid cellular response to ketone response to organic cyclic compound	cellular anatomical structure membrane cell periphery plasma membrane membrane microdomain membrane raft somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection intracellular anatomical structure cytoplasm cellular anatomical structure membrane cell periphery plasma membrane membrane microdomain membrane raft somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection intracellular anatomical structure cytoplasm protein-containing complex
C	Guanine nucleotide-binding protein G(s), alpha subunit	peptide hormone receptor binding protein binding binding corticotropin-releasing hormone receptor binding signaling receptor binding G protein-coupled receptor binding corticotropin-releasing hormone receptor 1 binding hormone receptor binding neuropeptide receptor binding mu-type opioid receptor binding opioid receptor binding beta-2 adrenergic receptor binding adrenergic receptor binding D1 dopamine receptor binding peptide hormone receptor binding protein binding binding corticotropin-releasing hormone receptor binding signaling receptor binding G protein-coupled receptor binding corticotropin-releasing hormone receptor 1 binding hormone receptor binding neuropeptide receptor binding mu-type opioid receptor binding opioid receptor binding beta-2 adrenergic receptor binding adrenergic receptor binding D1 dopamine receptor binding dopamine receptor binding protein-containing complex binding G-protein beta/gamma-subunit complex binding ionotropic glutamate receptor binding glutamate receptor binding enzyme regulator activity cyclase regulator activity enzyme activator activity cyclase activator activity molecular function activator activity molecular function regulator activity adenylate cyclase regulator activity adenylate cyclase activator activity guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cation binding metal ion binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity insulin-like growth factor receptor binding	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation adenylate cyclase-activating G protein-coupled receptor signaling pathway cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway cellular process signal transduction adenylate cyclase-activating adrenergic receptor signaling pathway adrenergic receptor signaling pathway cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation adenylate cyclase-activating G protein-coupled receptor signaling pathway cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway cellular process signal transduction adenylate cyclase-activating adrenergic receptor signaling pathway adrenergic receptor signaling pathway multicellular organismal process system process sensory perception of chemical stimulus sensory perception nervous system process cellular response to nitrogen compound response to dopamine cellular response to dopamine response to monoamine response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus response to organic cyclic compound G protein-coupled dopamine receptor signaling pathway cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to monoamine stimulus response to chemical	protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cellular anatomical structure cytoplasmic side of plasma membrane GTPase complex protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cellular anatomical structure cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane intracellular anatomical structure cytoplasm
A	Adenylate cyclase, type V	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding phosphorus-oxygen lyase activity cyclase activity lyase activity adenylate cyclase activity catalytic activity cation binding metal ion binding	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation adenylate cyclase-activating G protein-coupled receptor signaling pathway cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway cellular process signal transduction positive regulation of cytosolic calcium ion concentration regulation of biological quality cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation adenylate cyclase-activating G protein-coupled receptor signaling pathway cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway cellular process signal transduction positive regulation of cytosolic calcium ion concentration regulation of biological quality ribose phosphate metabolic process nucleotide biosynthetic process carbohydrate derivative metabolic process purine ribonucleotide biosynthetic process cAMP biosynthetic process cyclic nucleotide metabolic process nucleobase-containing compound metabolic process ribonucleotide biosynthetic process purine nucleotide metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine-containing compound biosynthetic process cyclic nucleotide biosynthetic process phosphate-containing compound metabolic process small molecule metabolic process phosphorus metabolic process ribose phosphate biosynthetic process nucleoside phosphate metabolic process carbohydrate derivative biosynthetic process cAMP metabolic process cyclic purine nucleotide metabolic process primary metabolic process nucleotide metabolic process nucleobase-containing compound biosynthetic process purine-containing compound metabolic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process nucleoside phosphate biosynthetic process metabolic process purine ribonucleotide metabolic process purine nucleotide biosynthetic process adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway intracellular signal transduction protein localization regulation of localization establishment of protein localization nitrogen compound transport localization carbohydrate homeostasis cellular macromolecule localization establishment of localization in cell regulation of peptide hormone secretion regulation of hormone levels establishment of protein localization to extracellular region intracellular glucose homeostasis protein secretion establishment of localization cellular response to oxygen-containing compound regulation of protein secretion regulation of secretion cellular response to carbohydrate stimulus chemical homeostasis response to glucose response to chemical glucose homeostasis insulin secretion involved in cellular response to glucose stimulus cellular localization regulation of hormone secretion regulation of protein localization regulation of peptide secretion transport homeostatic process hormone secretion protein localization to extracellular region peptide secretion secretion by cell regulation of secretion by cell response to monosaccharide response to carbohydrate amide transport signal release cellular response to hexose stimulus intracellular chemical homeostasis cellular response to monosaccharide stimulus regulation of signaling secretion regulation of transport regulation of peptide transport regulation of establishment of protein localization cell-cell signaling export from cell response to oxygen-containing compound cellular response to glucose stimulus regulation of protein transport peptide transport response to hexose cellular response to chemical stimulus cellular homeostasis protein transport regulation of cellular localization regulation of insulin secretion peptide hormone secretion macromolecule localization regulation of insulin secretion involved in cellular response to glucose stimulus hormone transport regulation of cell communication insulin secretion response to ketone response to lipid response to alcohol cellular response to forskolin cellular response to organic cyclic compound cellular response to alcohol response to forskolin cellular response to lipid cellular response to ketone response to organic cyclic compound	membrane-bounded organelle cell projection cellular anatomical structure organelle cilium plasma membrane bounded cell projection membrane cell periphery plasma membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B	IPR018297	Adenylyl cyclase class-4/guanylyl cyclase, conserved site	Conserved Site
B	IPR009398	Adenylate cyclase, conserved domain	Domain
B	IPR001054	Adenylyl cyclase class-3/4/guanylyl cyclase	Domain
B	IPR032628	Adenylate cyclase, N-terminal	Domain
B	IPR030672	Adenylate cyclase	Family
B	IPR029787	Nucleotide cyclase	Homologous Superfamily
C	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
C	IPR000367	G-protein alpha subunit, group S	Family
C	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
C	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A	IPR018297	Adenylyl cyclase class-4/guanylyl cyclase, conserved site	Conserved Site
A	IPR009398	Adenylate cyclase, conserved domain	Domain
A	IPR001054	Adenylyl cyclase class-3/4/guanylyl cyclase	Domain
A	IPR032628	Adenylate cyclase, N-terminal	Domain
A	IPR029787	Nucleotide cyclase	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.