2XS0

Linear binding motifs for JNK and for calcineurin antagonistically control the nuclear shuttling of NFAT4

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Protein kinase-like (PK-like)	8040900	3000066	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Pkinase_Tyr	e2xs0A1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
A	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00069	Protein kinase domain (Pkinase)	Protein kinase domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B	NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 3	DNA-binding transcription factor activity, RNA polymerase II-specific DNA-binding transcription repressor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription repressor activity DNA-binding transcription factor activity DNA binding sequence-specific DNA binding binding double-stranded DNA binding nucleic acid binding RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding DNA-binding transcription factor activity, RNA polymerase II-specific DNA-binding transcription repressor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription repressor activity DNA-binding transcription factor activity DNA binding sequence-specific DNA binding binding double-stranded DNA binding nucleic acid binding RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding organic cyclic compound binding transcription regulatory region nucleic acid binding RNA polymerase II cis-regulatory region sequence-specific DNA binding DNA-binding transcription activator activity, RNA polymerase II-specific DNA-binding transcription activator activity	developmental process multicellular organismal process regulation of biological process regulation of developmental process positive regulation of artery morphogenesis blood vessel morphogenesis tube development artery development biological regulation positive regulation of biological process vasculature development circulatory system development positive regulation of developmental process multicellular organism development anatomical structure morphogenesis developmental process multicellular organismal process regulation of biological process regulation of developmental process positive regulation of artery morphogenesis blood vessel morphogenesis tube development artery development biological regulation positive regulation of biological process vasculature development circulatory system development positive regulation of developmental process multicellular organism development anatomical structure morphogenesis tube morphogenesis regulation of artery morphogenesis anatomical structure development artery morphogenesis regulation of anatomical structure morphogenesis blood vessel development system development regulation of biosynthetic process regulation of nitric oxide biosynthetic process regulation of cellular process reactive nitrogen species metabolic process positive regulation of nitric oxide metabolic process nitric oxide metabolic process positive regulation of metabolic process nitric oxide biosynthetic process biosynthetic process positive regulation of biosynthetic process cellular process metabolic process regulation of nitric oxide metabolic process positive regulation of nitric oxide biosynthetic process regulation of metabolic process positive regulation of cellular process calcineurin-NFAT signaling cascade cellular response to stimulus response to stimulus signaling intracellular signal transduction cell communication calcium-mediated signaling intracellular signaling cassette signal transduction calcineurin-mediated signaling thymic T cell selection cellular developmental process hemopoiesis cell activation cell development mononuclear cell differentiation lymphocyte activation lymphocyte differentiation positive thymic T cell selection leukocyte activation T cell differentiation in thymus T cell activation immune system process cell differentiation positive T cell selection T cell differentiation T cell selection leukocyte differentiation positive regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death cell death positive regulation of apoptotic process apoptotic process programmed cell death DN4 thymocyte differentiation protein localization establishment of protein localization cellular localization nitrogen compound transport nucleocytoplasmic transport transport nuclear transport intracellular transport protein localization to nucleus establishment of localization localization import into nucleus establishment of protein localization to organelle protein transport cellular macromolecule localization macromolecule localization protein localization to organelle establishment of localization in cell protein import into nucleus intracellular protein transport negative regulation of biological process negative regulation of miRNA transcription RNA metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process RNA biosynthetic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process miRNA transcription regulation of miRNA metabolic process negative regulation of cellular process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process miRNA metabolic process negative regulation of macromolecule metabolic process regulation of RNA biosynthetic process negative regulation of DNA-templated transcription negative regulation of metabolic process primary metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of gene expression negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of miRNA metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription negative regulation of RNA metabolic process regulation of miRNA transcription muscle hypertrophy regulation of muscle adaptation system process positive regulation of cardiac muscle hypertrophy regulation of system process striated muscle hypertrophy regulation of muscle system process muscle system process regulation of cardiac muscle hypertrophy regulation of muscle hypertrophy muscle adaptation regulation of response to stimulus positive regulation of muscle hypertrophy cardiac muscle hypertrophy positive regulation of multicellular organismal process regulation of multicellular organismal process defense response response to stress inflammatory response smooth muscle cell differentiation regulation of smooth muscle cell differentiation negative regulation of muscle cell differentiation regulation of cell differentiation muscle cell differentiation muscle structure development vascular associated smooth muscle cell differentiation negative regulation of cell differentiation negative regulation of developmental process negative regulation of vascular associated smooth muscle cell differentiation regulation of vascular associated smooth muscle cell differentiation negative regulation of smooth muscle cell differentiation regulation of muscle cell differentiation transcription by RNA polymerase II regulation of transcription by RNA polymerase II positive regulation of DNA-templated transcription positive regulation of macromolecule biosynthetic process positive regulation of macromolecule metabolic process positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process positive regulation of RNA biosynthetic process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol protein-containing complex transcription regulator complex membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen cellular anatomical structure intracellular anatomical structure cytoplasm cytosol protein-containing complex transcription regulator complex membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen membraneless organelle intracellular membraneless organelle chromatin chromosome
A	MITOGEN-ACTIVATED PROTEIN KINASE 8	histone deacetylase binding protein binding binding enzyme binding enzyme regulator activity molecular function regulator activity histone deacetylase regulator activity protein phosphatase binding phosphatase binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding histone deacetylase binding protein binding binding enzyme binding enzyme regulator activity molecular function regulator activity histone deacetylase regulator activity protein phosphatase binding phosphatase binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding protein serine/threonine kinase activity JUN kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity MAP kinase activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein serine kinase activity protein serine/threonine kinase binding kinase binding protein kinase binding	regulation of protein binding regulation of molecular function negative regulation of protein binding negative regulation of binding regulation of binding biological regulation negative regulation of molecular function regulation of biological process catabolic process regulation of cellular process macroautophagy autophagy regulation of catabolic process regulation of autophagy regulation of protein binding regulation of molecular function negative regulation of protein binding negative regulation of binding regulation of binding biological regulation negative regulation of molecular function regulation of biological process catabolic process regulation of cellular process macroautophagy autophagy regulation of catabolic process regulation of autophagy regulation of macroautophagy cellular process metabolic process process utilizing autophagic mechanism regulation of metabolic process regulation of biosynthetic process positive regulation of biological process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process positive regulation of macromolecule biosynthetic process regulation of gene expression positive regulation of metabolic process biosynthetic process positive regulation of gene expression positive regulation of biosynthetic process regulation of macromolecule biosynthetic process positive regulation of macromolecule metabolic process macromolecule metabolic process positive regulation of cellular process positive regulation of molecular function positive regulation of cyclase activity regulation of catalytic activity positive regulation of catalytic activity regulation of cyclase activity positive regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death cell death positive regulation of apoptotic process apoptotic process programmed cell death protein phosphorylation phosphorylation primary metabolic process macromolecule modification peptidyl-amino acid modification phosphate-containing compound metabolic process peptidyl-threonine modification protein metabolic process protein modification process phosphorus metabolic process peptidyl-threonine phosphorylation cellular response to stimulus cellular response to stress response to stimulus response to stress cellular senescence response to oxidative stress cellular response to chemical stimulus response to chemical cellular response to oxidative stress cellular response to chemical stress protein localization regulation of localization establishment of protein localization cellular localization regulation of protein localization positive regulation of establishment of protein localization localization cellular macromolecule localization regulation of establishment of protein localization establishment of localization regulation of establishment of protein localization to mitochondrion establishment of protein localization to organelle regulation of cellular localization macromolecule localization protein localization to organelle positive regulation of protein localization protein localization to mitochondrion establishment of protein localization to mitochondrion positive regulation of establishment of protein localization to mitochondrion signaling intracellular signal transduction cell communication JNK cascade intracellular signaling cassette MAPK cascade signal transduction response to light stimulus response to radiation response to abiotic stimulus response to UV immune response-regulating cell surface receptor signaling pathway cell surface receptor signaling pathway immune response Fc receptor signaling pathway immune response-regulating signaling pathway regulation of response to stimulus immune system process Fc-epsilon receptor signaling pathway regulation of immune response regulation of immune system process peptidyl-serine modification peptidyl-serine phosphorylation response to nutrient levels cellular response to amino acid starvation cellular response to starvation cellular response to nutrient levels response to amino acid starvation response to starvation cellular response to metal ion response to metal ion cellular response to cadmium ion response to cadmium ion positive regulation of protein metabolic process regulation of protein metabolic process regulation of primary metabolic process JUN phosphorylation stress-activated MAPK cascade stress-activated protein kinase signaling cascade rhythmic process circadian rhythm regulation of circadian rhythm negative regulation of programmed cell death negative regulation of biological process negative regulation of apoptotic process negative regulation of cellular process biological process involved in interspecies interaction between organisms response to lipid response to other organism cellular response to lipopolysaccharide response to oxygen-containing compound response to external stimulus cellular response to molecule of bacterial origin response to lipopolysaccharide cellular response to oxygen-containing compound response to biotic stimulus cellular response to biotic stimulus response to bacterium cellular response to lipid response to external biotic stimulus response to molecule of bacterial origin multicellular organismal process multicellular organismal-level homeostasis homeostatic process energy homeostasis positive regulation of cell communication positive regulation of response to biotic stimulus positive regulation of signal transduction pattern recognition receptor signaling pathway regulation of NLRP3 inflammasome complex assembly positive regulation of NLRP3 inflammasome complex assembly regulation of signal transduction cellular component biogenesis immune response-activating signaling pathway cellular component assembly inflammasome-mediated signaling pathway positive regulation of immune response canonical inflammasome complex assembly positive regulation of immune system process innate immune response-activating signaling pathway positive regulation of pattern recognition receptor signaling pathway regulation of innate immune response positive regulation of intracellular signal transduction positive regulation of inflammasome-mediated signaling pathway regulation of inflammasome-mediated signaling pathway defense response to other organism regulation of pattern recognition receptor signaling pathway NLRP3 inflammasome complex assembly positive regulation of defense response activation of innate immune response inflammatory response activation of immune response cytoplasmic pattern recognition receptor signaling pathway regulation of defense response regulation of response to biotic stimulus regulation of cellular component organization positive regulation of cellular component organization regulation of response to external stimulus intracellular receptor signaling pathway regulation of cytoplasmic pattern recognition receptor signaling pathway positive regulation of response to stimulus cellular component organization regulation of protein-containing complex assembly positive regulation of protein-containing complex assembly positive regulation of response to external stimulus regulation of intracellular signal transduction regulation of response to stress innate immune response protein-containing complex assembly cellular component organization or biogenesis regulation of signaling positive regulation of innate immune response defense response protein-containing complex organization positive regulation of cellular component biogenesis defense response to symbiont regulation of cellular component biogenesis regulation of cell communication positive regulation of signaling response to mechanical stimulus response to reactive oxygen species cellular response to reactive oxygen species cellular response to mechanical stimulus cellular response to abiotic stimulus cellular response to external stimulus cellular response to environmental stimulus regulation of cell killing positive regulation of cell killing cell killing	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol basal dendrite somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection axon membrane-bounded organelle nucleus cellular anatomical structure intracellular anatomical structure cytoplasm cytosol basal dendrite somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection axon membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen synapse cell junction

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B	IPR037059	Rel homology domain (RHD), DNA-binding domain superfamily	Homologous Superfamily
B	IPR002909	IPT domain	Domain
B	IPR008967	p53-like transcription factor, DNA-binding domain superfamily	Homologous Superfamily
B	IPR014756	Immunoglobulin E-set	Homologous Superfamily
B	IPR011539	Rel homology domain, DNA-binding domain	Domain
B	IPR008366	Nuclear factor of activated T cells (NFAT)	Family
B	IPR032397	Rel homology dimerisation domain	Domain
B	IPR013783	Immunoglobulin-like fold	Homologous Superfamily
A	IPR003527	Mitogen-activated protein (MAP) kinase, conserved site	Conserved Site
A	IPR000719	Protein kinase domain	Domain
A	IPR050117	Mitogen-activated protein kinase	Family
A	IPR008351	Mitogen-activated protein (MAP) kinase, JNK	Family
A	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A	IPR008271	Serine/threonine-protein kinase, active site	Active Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B	Pharos: Q12968	atypical teratoid rhabdoid tumor medulloblastoma pancreatic ductal adenocarcinoma psoriasis large cell medulloblastoma osteosarcoma Pick disease Sjogren syndrome malignant mesothelioma ovarian cancer primary biliary cholangitis tuberculosis
A	Pharos: P45983	acute liver failure Hyperalgesia trigeminal neuralgia extrahepatic cholestasis glioblastoma ischemia reperfusion injury ovarian cancer atopic eczema precancerous condition cancer schizophrenia gastric cancer hereditary diffuse gastric adenocarcinoma malignant ependymoma acute myeloid leukemia acute liver failure Hyperalgesia trigeminal neuralgia extrahepatic cholestasis glioblastoma ischemia reperfusion injury ovarian cancer atopic eczema precancerous condition cancer schizophrenia gastric cancer hereditary diffuse gastric adenocarcinoma malignant ependymoma acute myeloid leukemia pancreatic intraductal papillary-mucinous carcinoma atypical teratoid rhabdoid tumor depressive disorder thrombocytopenia due to immune destruction tuberculosis gastric neoplasm malignant glioma medulloblastoma osteosarcoma pilocytic astrocytoma breast cancer spina bifida dermatitis, atopic

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.