3OOJ

C1A mutant of E. coli GlmS in complex with glucose-6P and glutamate

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
D	d3oojd2	Alpha and beta proteins (a/b)	SIS domain	SIS domain	double-SIS domain	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D	d3oojd1	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	automated matches	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
E	d3ooje2	Alpha and beta proteins (a/b)	SIS domain	SIS domain	double-SIS domain	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
E	d3ooje1	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	automated matches	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d3ooja2	Alpha and beta proteins (a/b)	SIS domain	SIS domain	double-SIS domain	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d3ooja1	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	automated matches	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d3oojb2	Alpha and beta proteins (a/b)	SIS domain	SIS domain	double-SIS domain	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d3oojb1	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	automated matches	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d3oojc2	Alpha and beta proteins (a/b)	SIS domain	SIS domain	double-SIS domain	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d3oojc1	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	automated matches	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
F	d3oojf2	Alpha and beta proteins (a/b)	SIS domain	SIS domain	double-SIS domain	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
F	d3oojf1	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	automated matches	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
G	d3oojg2	Alpha and beta proteins (a/b)	SIS domain	SIS domain	double-SIS domain	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
G	d3oojg1	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	automated matches	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
H	d3oojh2	Alpha and beta proteins (a/b)	SIS domain	SIS domain	double-SIS domain	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
H	d3oojh1	Alpha and beta proteins (a+b)	Ntn hydrolase-like	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	automated matches	automated matches	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
D	SCOP2B Superfamily	Class II glutamine amidotransferases	8041848	3000131	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8032993	3001397	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8052796	3001397	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8032993	3001397	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Class II glutamine amidotransferases	8041848	3000131	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8052796	3001397	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8032993	3001397	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Class II glutamine amidotransferases	8041848	3000131	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8052796	3001397	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Class II glutamine amidotransferases	8041848	3000131	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8032993	3001397	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8052796	3001397	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8032993	3001397	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Class II glutamine amidotransferases	8041848	3000131	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8052796	3001397	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Class II glutamine amidotransferases	8041848	3000131	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8052796	3001397	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8032993	3001397	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Class II glutamine amidotransferases	8041848	3000131	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8052796	3001397	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8032993	3001397	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8052796	3001397	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8032993	3001397	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Class II glutamine amidotransferases	8041848	3000131	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
D	GATase_2_1st	e3oojD6	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Class II glutamine amidotransferases	F: GATase_2_1st	ECOD (1.6)
D	SIS_3	e3oojD3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: SIS_3	ECOD (1.6)
E	GATase_2_1st	e3oojE6	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Class II glutamine amidotransferases	F: GATase_2_1st	ECOD (1.6)
E	SIS_3	e3oojE3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: SIS_3	ECOD (1.6)
A	GATase_2_1st	e3oojA1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Class II glutamine amidotransferases	F: GATase_2_1st	ECOD (1.6)
A	SIS_3	e3oojA3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: SIS_3	ECOD (1.6)
B	GATase_2_1st	e3oojB1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Class II glutamine amidotransferases	F: GATase_2_1st	ECOD (1.6)
B	SIS_3	e3oojB3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: SIS_3	ECOD (1.6)
C	GATase_2_1st	e3oojC8	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Class II glutamine amidotransferases	F: GATase_2_1st	ECOD (1.6)
C	SIS_3	e3oojC3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: SIS_3	ECOD (1.6)
F	GATase_2_1st	e3oojF1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Class II glutamine amidotransferases	F: GATase_2_1st	ECOD (1.6)
F	SIS_3	e3oojF3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: SIS_3	ECOD (1.6)
G	GATase_2_1st	e3oojG1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Class II glutamine amidotransferases	F: GATase_2_1st	ECOD (1.6)
G	SIS_3	e3oojG3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: SIS_3	ECOD (1.6)
H	GATase_2_1st	e3oojH1	A: a+b four layers	X: Ntn/PP2C	H: Ntn	T: Class II glutamine amidotransferases	F: GATase_2_1st	ECOD (1.6)
H	SIS_3	e3oojH3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: SIS_3	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
D	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
D	3.40.50.10490	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Glucose-6-phosphate isomerase like protein	CATH (4.3.0)
E	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
E	3.40.50.10490	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Glucose-6-phosphate isomerase like protein	CATH (4.3.0)
A	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
A	3.40.50.10490	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Glucose-6-phosphate isomerase like protein	CATH (4.3.0)
B	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
B	3.40.50.10490	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Glucose-6-phosphate isomerase like protein	CATH (4.3.0)
C	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
C	3.40.50.10490	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Glucose-6-phosphate isomerase like protein	CATH (4.3.0)
F	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
F	3.40.50.10490	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Glucose-6-phosphate isomerase like protein	CATH (4.3.0)
G	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
G	3.40.50.10490	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Glucose-6-phosphate isomerase like protein	CATH (4.3.0)
H	3.60.20.10	Alpha Beta	4-Layer Sandwich	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	Aminohydrolase, N-terminal nucleophile (Ntn) domain	CATH (4.3.0)
H	3.40.50.10490	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Glucose-6-phosphate isomerase like protein	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H	PF01380	SIS domain (SIS)	SIS domain	SIS (Sugar ISomerase) domains are found in many phosphosugar isomerases and phosphosugar binding proteins. SIS domains are also found in proteins that regulate the expression of genes involved in synthesis of phosphosugars. Presumably the SIS domain ...	SIS (Sugar ISomerase) domains are found in many phosphosugar isomerases and phosphosugar binding proteins. SIS domains are also found in proteins that regulate the expression of genes involved in synthesis of phosphosugars. Presumably the SIS domains bind to the end-product of the pathway.	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	PF13522	Glutamine amidotransferase domain (GATase_6)	Glutamine amidotransferase domain	This domain is a class-II glutamine amidotransferase domain found in a variety of enzymes, such as asparagine synthetase and glutamine--fructose-6-phosphate transaminase.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H	Glucosamine/fructose-6-phosphate aminotransferase, isomerizing	binding carbohydrate derivative binding transferase activity, transferring nitrogenous groups glutamine-fructose-6-phosphate transaminase (isomerizing) activity L-glutamine aminotransferase activity transaminase activity transferase activity catalytic activity	carbohydrate derivative biosynthetic process glycoprotein biosynthetic process carbohydrate derivative metabolic process primary metabolic process glycoprotein metabolic process protein N-linked glycosylation protein glycosylation macromolecule biosynthetic process biosynthetic process glycosylation macromolecule modification macromolecule glycosylation metabolic process cellular process carbohydrate derivative biosynthetic process glycoprotein biosynthetic process carbohydrate derivative metabolic process primary metabolic process glycoprotein metabolic process protein N-linked glycosylation protein glycosylation macromolecule biosynthetic process biosynthetic process glycosylation macromolecule modification macromolecule glycosylation metabolic process cellular process protein metabolic process macromolecule metabolic process protein modification process organophosphate metabolic process phosphate-containing compound metabolic process fructose 6-phosphate metabolic process phosphorus metabolic process carbohydrate metabolic process nucleoside phosphate metabolic process nucleotide-sugar metabolic process nucleobase-containing compound metabolic process nucleobase-containing small molecule metabolic process amino sugar metabolic process small molecule metabolic process UDP-N-acetylglucosamine metabolic process amino sugar biosynthetic process nucleobase-containing compound biosynthetic process UDP-N-acetylglucosamine biosynthetic process organophosphate biosynthetic process nucleoside phosphate biosynthetic process nucleotide-sugar biosynthetic process glutamine metabolic process oxoacid metabolic process carboxylic acid metabolic process amino acid metabolic process organic acid metabolic process alpha-amino acid metabolic process L-amino acid metabolic process glutamine family amino acid metabolic process proteinogenic amino acid metabolic process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H	IPR046348	SIS domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR029055	Nucleophile aminohydrolases, N-terminal	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H	IPR035466	GlmS/AgaS, SIS domain 1	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR005855	Glucosamine-fructose-6-phosphate aminotransferase, isomerising	Family
A, B, C, D, E A, B, C, D, E, F, G, H	IPR035490	GlmS/FrlB, SIS domain 2	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR017932	Glutamine amidotransferase type 2 domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR047084	Glucosamine-fructose-6-phosphate aminotransferase, isomerising, N-terminal domain	Domain
A, B, C, D, E A, B, C, D, E, F, G, H	IPR001347	SIS domain	Domain