3QSK

5 Histidine Variant of the anti-RNase A VHH in Complex with RNAse A

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
IMGT Antibody Annotation
SAbDab Antibody Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3qska_	Alpha and beta proteins (a+b)	RNase A-like	RNase A-like	Ribonuclease A-like	automated matches	(Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
B	d3qskb_	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	V set domains (antibody variable domain-like)	automated matches	Arabian camel (Camelus dromedarius ) [TaxId: 9838 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	RNase A-like	8038165	3001918	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	RnaseA	e3qskA1	A: a+b complex topology	X: RNase A-like	H: RNase A-like (From Topology)	T: RNase A-like	F: RnaseA	ECOD (1.6)
B	V-set_8	e3qskB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_8	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.130.10	Alpha Beta	Roll	P-30 Protein	Ribonuclease A-like domain	CATH (4.3.0)
B	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)

IMGT Antibody Annotation IMGT Database Homepage

Chain	Protein Name	Description	Organism Name	Gene Allele Name(s)	Domain Name(s)	Receptor Type	Receptor Description	Provenance Source
B	Engineered 5 Histidine anti-RNase A llama heavy chain VH domain	VH	Camelus dromedarius (Arabian camel)	IGHV3S4501, IGHJ301	V-DOMAIN VH	IG	(VH)	IMGT (202445-0)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
B	Heavy Chain	IGHV1	-	ribonuclease pancreatic	SAbDab (2024-11-15)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00074	Pancreatic ribonuclease (RnaseA)	Pancreatic ribonuclease	Ribonucleases. Members include pancreatic RNAase A and angiogenins. Structure is an alpha+beta fold -- long curved beta sheet and three helices.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Ribonuclease pancreatic	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters endonuclease activity ribonuclease A activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity RNA endonuclease activity, producing 3'-phosphomonoesters nuclease activity hydrolase activity, acting on ester bonds RNA endonuclease activity catalytic activity lyase activity binding endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters endonuclease activity ribonuclease A activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity RNA endonuclease activity, producing 3'-phosphomonoesters nuclease activity hydrolase activity, acting on ester bonds RNA endonuclease activity catalytic activity lyase activity binding organic cyclic compound binding nucleic acid binding	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to Gram-positive bacterium response to external stimulus response to biotic stimulus defense response to bacterium defense response to other organism response to stress response to bacterium response to external biotic stimulus	cellular anatomical structure extracellular region
B	Engineered 5 Histidine anti-RNase A Camelid VHH Antibody Domain Variant	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR023412	Ribonuclease A-domain	Domain
A	IPR036816	Ribonuclease A-like domain superfamily	Homologous Superfamily
A	IPR001427	Pancreatic ribonuclease	Family
A	IPR023411	Ribonuclease A, active site	Active Site

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.