4CU8

Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4cu8a2	All beta proteins	Immunoglobulin-like beta-sandwich	beta-Galactosidase/glucuronidase domain	beta-Galactosidase/glucuronidase domain	beta-Galactosidase, domains 2 and 4	(Streptococcus pneumoniae TIGR4 ) [TaxId: 170187 ],	SCOPe (2.08)
A	d4cu8a4	All beta proteins	Immunoglobulin-like beta-sandwich	E set domains	E-set domains of sugar-utilizing enzymes	Beta-galactosidase	(Streptococcus pneumoniae TIGR4 ) [TaxId: 170187 ],	SCOPe (2.08)
A	d4cu8a1	All beta proteins	Galactose-binding domain-like	Galactose-binding domain-like	beta-Galactosidase/glucuronidase, N-terminal domain	beta-Galactosidase	(Streptococcus pneumoniae TIGR4 ) [TaxId: 170187 ],	SCOPe (2.08)
A	d4cu8a3	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	(Trans)glycosidases	beta-glycanases	beta-Galactosidase, domain 3	(Streptococcus pneumoniae TIGR4 ) [TaxId: 170187 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	E set domains	8090618	3000070	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	beta-Galactosidase/glucuronidase domain-like	8090612	3002047	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	(Trans)glycosidases	8090614	3000313	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	E set domains	8090616	3000070	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	galactose-binding domain-like	8090610	3000087	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Glyco_hydro_2_N	e4cu8A3	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Galactose-binding domain-like	F: Glyco_hydro_2_N	ECOD (1.6)
A	Glyco_hydro_2	e4cu8A2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: Glyco_hydro_2	ECOD (1.6)
A	Invasin_D3_2	e4cu8A1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: Invasin_D3_2	ECOD (1.6)
A	DUF4982	e4cu8A5	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: DUF4982	ECOD (1.6)
A	Glyco_hydro_2_C	e4cu8A4	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glyco_hydro_2_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.120.260	Mainly Beta	Sandwich	Jelly Rolls	Galactose-binding domain-like	CATH (4.3.0)
A	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
A	3.20.20.80	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Glycosidases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF02836	Glycosyl hydrolases family 2, TIM barrel domain (Glyco_hydro_2_C)	Glycosyl hydrolases family 2, TIM barrel domain	This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.	Domain
A	PF00703	Glycosyl hydrolases family 2 (Glyco_hydro_2)	Glycosyl hydrolases family 2	This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.	Domain
A	PF02837	Glycosyl hydrolases family 2, sugar binding domain (Glyco_hydro_2_N)	Glycosyl hydrolases family 2, sugar binding domain	This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.	Domain
A	PF18565	Glycoside hydrolase family 2 C-terminal domain 5 (Glyco_hydro2_C5)	Glycoside hydrolase family 2 C-terminal domain 5	Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a fun ...	Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme [1].	Domain
A	PF16355	Domain of unknown function (DUF4982) (DUF4982)	Domain of unknown function (DUF4982)	This domain is found towards the C terminus of Beta-glucuronidase from Formosa agariphila (P17_GH2) and similar proteins mainly from bacteria and fungi. P17_GH2 is involved in the degradation of the polysaccharide ulvan. The function of this domain, ...	This domain is found towards the C terminus of Beta-glucuronidase from Formosa agariphila (P17_GH2) and similar proteins mainly from bacteria and fungi. P17_GH2 is involved in the degradation of the polysaccharide ulvan. The function of this domain, which shows an all-beta structure, is unknown [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	GLYCOSIDE HYDROLASE 2	hydrolase activity, acting on glycosyl bonds beta-galactosidase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity catalytic activity galactosidase activity	carbohydrate metabolic process primary metabolic process metabolic process cellular process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR032311	Domain of unknown function DUF4982	Domain
A	IPR051913	Glycosyl Hydrolase 2 Domain-Containing Protein	Family
A	IPR017853	Glycoside hydrolase superfamily	Homologous Superfamily
A	IPR006101	Glycoside hydrolase, family 2	Family
A	IPR040605	Glycoside hydrolase family 2, domain 5	Domain
A	IPR008979	Galactose-binding-like domain superfamily	Homologous Superfamily
A	IPR006102	Glycoside hydrolase, family 2, immunoglobulin-like beta-sandwich	Domain
A	IPR036156	Beta-Galactosidase/glucuronidase domain superfamily	Homologous Superfamily
A	IPR006104	Glycosyl hydrolases family 2, sugar binding domain	Domain
A	IPR013783	Immunoglobulin-like fold	Homologous Superfamily
A	IPR005877	YSIRK Gram-positive signal peptide	Domain
A	IPR011098	G5 domain	Domain
A	IPR006103	Glycoside hydrolase family 2, catalytic domain	Domain
A	IPR049487	Beta-galactosidase-like, galactose-binding domain	Domain
A	IPR011081	Bacterial Ig-like domain	Domain
A	IPR019931	LPXTG cell wall anchor domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.