5XT6

A sulfur-transferring catalytic intermediate of SufS-SufU complex from Bacillus subtilis

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth D]	SCOP2B Superfamily	SufE/NifU	8043955	3000436	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	PLP-dependent transferases	8074690	3000954	SCOP2B (2022-06-29)
B [auth A]	SCOP2B Superfamily	PLP-dependent transferases	8074690	3000954	SCOP2B (2022-06-29)
D [auth C]	SCOP2B Superfamily	SufE/NifU	8043955	3000436	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth D]	NifU_N	e5xt6D1	A: a+b two layers	X: FAD-linked reductases, C-terminal domain-like	H: SufE/NifU (From Topology)	T: SufE/NifU	F: NifU_N	ECOD (1.6)
C [auth B]	Aminotran_5_C	e5xt6B1	A: a+b two layers	X: C-terminal domain in some PLP-dependent transferases (From Topology)	H: C-terminal domain in some PLP-dependent transferases (From Topology)	T: C-terminal domain in some PLP-dependent transferases	F: Aminotran_5_C	ECOD (1.6)
C [auth B]	Aminotran_5_N	e5xt6B2	A: a/b three-layered sandwiches	X: PLP-dependent transferases (From Topology)	H: PLP-dependent transferases (From Topology)	T: PLP-dependent transferases	F: Aminotran_5_N	ECOD (1.6)
B [auth A]	Aminotran_5_C	e5xt6A1	A: a+b two layers	X: C-terminal domain in some PLP-dependent transferases (From Topology)	H: C-terminal domain in some PLP-dependent transferases (From Topology)	T: C-terminal domain in some PLP-dependent transferases	F: Aminotran_5_C	ECOD (1.6)
B [auth A]	Aminotran_5_N	e5xt6A2	A: a/b three-layered sandwiches	X: PLP-dependent transferases (From Topology)	H: PLP-dependent transferases (From Topology)	T: PLP-dependent transferases	F: Aminotran_5_N	ECOD (1.6)
D [auth C]	NifU_N	e5xt6C1	A: a+b two layers	X: FAD-linked reductases, C-terminal domain-like	H: SufE/NifU (From Topology)	T: SufE/NifU	F: NifU_N	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth D]	3.90.1010.10	Alpha Beta	Alpha-Beta Complex	Sufe protein. Chain: A		CATH (4.3.0)
C [auth B]	3.40.640.10	Alpha Beta	3-Layer(aba) Sandwich	Aspartate Aminotransferase	domain 2	CATH (utative)
B [auth A]	3.40.640.10	Alpha Beta	3-Layer(aba) Sandwich	Aspartate Aminotransferase	domain 2	CATH (utative)
D [auth C]	3.90.1010.10	Alpha Beta	Alpha-Beta Complex	Sufe protein. Chain: A		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth D]	PF01592	NifU-like N terminal domain (NifU_N)	NifU-like N terminal domain	-	Family
B [auth A], C [auth B]	PF00266	Aminotransferase class-V (Aminotran_5)	Aminotransferase class-V	This domain is found in amino transferases, and other enzymes including cysteine desulphurase EC:4.4.1.-.	Domain
D [auth C]	PF01592	NifU-like N terminal domain (NifU_N)	NifU-like N terminal domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth D]	Zinc-dependent sulfurtransferase SufU	cation binding transition metal ion binding iron ion binding ion binding binding ferrous iron binding metal ion binding small molecule binding iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding metal cluster binding transferase activity catalytic activity	intracellular iron ion homeostasis inorganic ion homeostasis cellular homeostasis chemical homeostasis homeostatic process intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis cellular component biogenesis cellular component organization metallo-sulfur cluster assembly cellular component assembly intracellular iron ion homeostasis inorganic ion homeostasis cellular homeostasis chemical homeostasis homeostatic process intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis cellular component biogenesis cellular component organization metallo-sulfur cluster assembly cellular component assembly iron-sulfur cluster assembly cellular process cellular component organization or biogenesis	cellular anatomical structure intracellular anatomical structure cytoplasm
B [auth A], C [auth B]	Cysteine desulfurase SufS	cysteine desulfurase activity sulfurtransferase activity transferase activity, transferring sulphur-containing groups transferase activity catalytic activity vitamin binding vitamin B6 binding pyridoxal phosphate binding ion binding binding organic cyclic compound binding anion binding heterocyclic compound binding small molecule binding	oxoacid metabolic process sulfur amino acid metabolic process carboxylic acid metabolic process amino acid metabolic process primary metabolic process organic acid metabolic process alpha-amino acid metabolic process cysteine metabolic process metabolic process cellular process small molecule metabolic process sulfur compound metabolic process	-
D [auth C]	Zinc-dependent sulfurtransferase SufU	cation binding transition metal ion binding iron ion binding ion binding binding ferrous iron binding metal ion binding small molecule binding iron-sulfur cluster binding 2 iron, 2 sulfur cluster binding metal cluster binding transferase activity catalytic activity	intracellular iron ion homeostasis inorganic ion homeostasis cellular homeostasis chemical homeostasis homeostatic process intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis cellular component biogenesis cellular component organization metallo-sulfur cluster assembly cellular component assembly intracellular iron ion homeostasis inorganic ion homeostasis cellular homeostasis chemical homeostasis homeostatic process intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis cellular component biogenesis cellular component organization metallo-sulfur cluster assembly cellular component assembly iron-sulfur cluster assembly cellular process cellular component organization or biogenesis	cellular anatomical structure intracellular anatomical structure cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth D]	IPR002871	NIF system FeS cluster assembly, NifU, N-terminal	Domain
B [auth A], C [auth B]	IPR020578	Aminotransferase class-V, pyridoxal-phosphate binding site	Binding Site
B [auth A], C [auth B]	IPR015424	Pyridoxal phosphate-dependent transferase	Homologous Superfamily
B [auth A], C [auth B]	IPR016454	Cysteine desulfurase	Family
B [auth A], C [auth B]	IPR015421	Pyridoxal phosphate-dependent transferase, major domain	Homologous Superfamily
B [auth A], C [auth B]	IPR010970	Cysteine desulfurase, SufS	Family
B [auth A], C [auth B]	IPR015422	Pyridoxal phosphate-dependent transferase, small domain	Homologous Superfamily
B [auth A], C [auth B]	IPR000192	Aminotransferase class V domain	Domain
D [auth C]	IPR002871	NIF system FeS cluster assembly, NifU, N-terminal	Domain

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
B [auth A], C [auth B]	CSS	Parent Component: CYS RESID: AA0269 PSI-MOD : L-cysteine persulfide MOD:00274
D [auth C]	CSS	Parent Component: CYS RESID: AA0269 , AA0269 PSI-MOD : L-cysteine persulfide MOD:00274 , L-cysteine persulfide MOD:00274

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.