5NG5

multi-drug efflux; membrane transport; RND superfamily; Drug resistance

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
AMR Gene: CARD Annotation
AMR Gene Family: CARD Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
G [auth F]	SCOP2B Superfamily	Outer membrane efflux proteins (OEP)	8037933	3000246	SCOP2B (2022-06-29)
I [auth C]	SCOP2B Superfamily	Outer membrane efflux proteins (OEP)	8037933	3000246	SCOP2B (2022-06-29)
H [auth I]	SCOP2B Superfamily	Outer membrane efflux proteins (OEP)	8037933	3000246	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042531	3000526	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042528	3000503	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042533	3000526	SCOP2B (2022-06-29)
J [auth K]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042523	3000503	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042523	3000503	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042531	3000526	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042528	3000503	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042533	3000526	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
K [auth L]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042523	3000503	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042531	3000526	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042528	3000503	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042533	3000526	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
O [auth J]	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
L [auth M]	SCOP2B Superfamily	AcrZ-like	8090987	3002474	SCOP2B (2022-06-29)
M [auth N]	SCOP2B Superfamily	AcrZ-like	8090987	3002474	SCOP2B (2022-06-29)
N [auth O]	SCOP2B Superfamily	AcrZ-like	8090987	3002474	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth G]	PRK15030_2nd	e5ng5G4	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
C [auth G]	HlyD_D23_C_1	e5ng5G2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
C [auth G]	HlyD_2nd	e5ng5G1	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
C [auth G]	HlyD_1st	e5ng5G3	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
D [auth H]	PRK15030_2nd	e5ng5H4	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
D [auth H]	HlyD_D23_C_1	e5ng5H3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
D [auth H]	HlyD_2nd	e5ng5H2	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
D [auth H]	HlyD_1st	e5ng5H1	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
E [auth A]	PRK15030_2nd	e5ng5A4	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
E [auth A]	HlyD_D23_C_1	e5ng5A3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
E [auth A]	HlyD_2nd	e5ng5A1	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
E [auth A]	HlyD_1st	e5ng5A2	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
F [auth B]	PRK15030_2nd	e5ng5B3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
F [auth B]	HlyD_D23_C_1	e5ng5B4	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
F [auth B]	HlyD_2nd	e5ng5B1	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
F [auth B]	HlyD_1st	e5ng5B2	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
A [auth E]	PRK15030_2nd	e5ng5E3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
A [auth E]	HlyD_D23_C_1	e5ng5E1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
A [auth E]	HlyD_2nd	e5ng5E4	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
A [auth E]	HlyD_1st	e5ng5E2	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
B [auth D]	PRK15030_2nd	e5ng5D4	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
B [auth D]	HlyD_D23_C_1	e5ng5D1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
B [auth D]	HlyD_2nd	e5ng5D2	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
B [auth D]	HlyD_1st	e5ng5D3	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
G [auth F]	OEP	e5ng5F1	A: a+b duplicates or obligate multimers	X: Outer membrane efflux proteins (OEP) (From Topology)	H: Outer membrane efflux proteins (OEP) (From Topology)	T: Outer membrane efflux proteins (OEP)	F: OEP	ECOD (1.6)
I [auth C]	OEP	e5ng5C1	A: a+b duplicates or obligate multimers	X: Outer membrane efflux proteins (OEP) (From Topology)	H: Outer membrane efflux proteins (OEP) (From Topology)	T: Outer membrane efflux proteins (OEP)	F: OEP	ECOD (1.6)
H [auth I]	OEP	e5ng5I1	A: a+b duplicates or obligate multimers	X: Outer membrane efflux proteins (OEP) (From Topology)	H: Outer membrane efflux proteins (OEP) (From Topology)	T: Outer membrane efflux proteins (OEP)	F: OEP	ECOD (1.6)
J [auth K]	ACR_tran_4th	e5ng5K7	A: alpha bundles	X: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	H: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	T: Multidrug efflux transporter AcrB transmembrane domain	F: ACR_tran_4th	ECOD (1.6)
J [auth K]	ACR_tran_1st	e5ng5K6	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_1st	ECOD (1.6)
J [auth K]	ACR_tran_5th	e5ng5K4	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_5th	ECOD (1.6)
J [auth K]	ACR_tran_3rd	e5ng5K2	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_3rd	ECOD (1.6)
J [auth K]	ACR_tran_6th	e5ng5K5	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_6th	ECOD (1.6)
J [auth K]	ACR_tran_2nd	e5ng5K1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_2nd	ECOD (1.6)
J [auth K]	ACR_tran_6th_1	e5ng5K3	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_6th_1	ECOD (1.6)
K [auth L]	ACR_tran_4th	e5ng5L5	A: alpha bundles	X: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	H: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	T: Multidrug efflux transporter AcrB transmembrane domain	F: ACR_tran_4th	ECOD (1.6)
K [auth L]	ACR_tran_1st	e5ng5L6	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_1st	ECOD (1.6)
K [auth L]	ACR_tran_5th	e5ng5L3	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_5th	ECOD (1.6)
K [auth L]	ACR_tran_3rd	e5ng5L8	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_3rd	ECOD (1.6)
K [auth L]	ACR_tran_6th	e5ng5L2	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_6th	ECOD (1.6)
K [auth L]	ACR_tran_2nd	e5ng5L1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_2nd	ECOD (1.6)
K [auth L]	ACR_tran_6th_1	e5ng5L4	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_6th_1	ECOD (1.6)
O [auth J]	ACR_tran_4th	e5ng5J6	A: alpha bundles	X: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	H: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	T: Multidrug efflux transporter AcrB transmembrane domain	F: ACR_tran_4th	ECOD (1.6)
O [auth J]	ACR_tran_1st	e5ng5J5	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_1st	ECOD (1.6)
O [auth J]	ACR_tran_5th	e5ng5J1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_5th	ECOD (1.6)
O [auth J]	ACR_tran_3rd	e5ng5J2	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_3rd	ECOD (1.6)
O [auth J]	ACR_tran_6th	e5ng5J3	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_6th	ECOD (1.6)
O [auth J]	ACR_tran_2nd	e5ng5J7	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_2nd	ECOD (1.6)
O [auth J]	ACR_tran_6th_1	e5ng5J4	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_6th_1	ECOD (1.6)
L [auth M]	AcrZ	e5ng5M1	A: extended segments	X: Multidrug efflux pump accessory protein AcrZ (From Topology)	H: Multidrug efflux pump accessory protein AcrZ (From Topology)	T: Multidrug efflux pump accessory protein AcrZ	F: AcrZ	ECOD (1.6)
M [auth N]	AcrZ	e5ng5N1	A: extended segments	X: Multidrug efflux pump accessory protein AcrZ (From Topology)	H: Multidrug efflux pump accessory protein AcrZ (From Topology)	T: Multidrug efflux pump accessory protein AcrZ	F: AcrZ	ECOD (1.6)
N [auth O]	AcrZ	e5ng5O1	A: extended segments	X: Multidrug efflux pump accessory protein AcrZ (From Topology)	H: Multidrug efflux pump accessory protein AcrZ (From Topology)	T: Multidrug efflux pump accessory protein AcrZ	F: AcrZ	ECOD (1.6)

AMR Gene: CARD Annotation CARD Database Homepage

Chains	Accession	Short Name	Description	Provenance Source (Version)
G [auth F], H [auth I], I [auth C]	ARO:3000237	TolC	TolC is a protein subunit of many multidrug efflux complexes in Gram negative bacteria. It is an outer membrane efflux protein and is constitutively open. Regulation of efflux activity is often at its periplasmic entrance by other components of the efflux complex.	CARD (3.3.0)
J [auth K], K [auth L], O [auth J]	ARO:3000216	acrB	Protein subunit of AcrA-AcrB-TolC multidrug efflux complex. AcrB functions as a herterotrimer which forms the inner membrane component and is primarily responsible for substrate recognition and energy transduction by acting as a drug/proton antiporter.	CARD (3.3.0)

AMR Gene Family: CARD Annotation CARD Database Homepage

Chains	Accession	Gene Family	Drug Class	Resistance Machanism	Provenance Source (Version)
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
G [auth F], H [auth I], I [auth C]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	macrolide antibiotic, fluoroquinolone antibiotic, aminoglycoside antibiotic, carbapenem, cephalosporin, glycylcycline, cephamycin, penam, tetracycline antibiotic, peptide antibiotic, aminocoumarin antibiotic, rifamycin antibiotic, phenicol antibiotic, penem, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
G [auth F], H [auth I], I [auth C]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	macrolide antibiotic, fluoroquinolone antibiotic, aminoglycoside antibiotic, carbapenem, cephalosporin, glycylcycline, cephamycin, penam, tetracycline antibiotic, peptide antibiotic, aminocoumarin antibiotic, rifamycin antibiotic, phenicol antibiotic, penem, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
G [auth F], H [auth I], I [auth C]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	macrolide antibiotic, fluoroquinolone antibiotic, aminoglycoside antibiotic, carbapenem, cephalosporin, glycylcycline, cephamycin, penam, tetracycline antibiotic, peptide antibiotic, aminocoumarin antibiotic, rifamycin antibiotic, phenicol antibiotic, penem, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
J [auth K], K [auth L], O [auth J]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
J [auth K], K [auth L], O [auth J]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
J [auth K], K [auth L], O [auth J]	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	PF16576	Barrel-sandwich domain of CusB or HlyD membrane-fusion (HlyD_D23)	Barrel-sandwich domain of CusB or HlyD membrane-fusion	HlyD_D23 is the combined domains 2 and 3 of the membrane-fusion proteins CusB and HlyD, which forms a barrel-sandwich. CusB and HlyD proteins are membrane fusion proteins of the CusCFBA copper efflux system in E.coli and related bacteria. The whole ...	HlyD_D23 is the combined domains 2 and 3 of the membrane-fusion proteins CusB and HlyD, which forms a barrel-sandwich. CusB and HlyD proteins are membrane fusion proteins of the CusCFBA copper efflux system in E.coli and related bacteria. The whole molecule hinges between D2 and D3. Efflux systems of this resistance-nodulation-division group - RND - have been developed to excrete poisonous metal ions, and in E.coli the only one that deals with silver and copper is the CusA transporter. The transporter CusA works in conjunction with a periplasmic component that is a membrane fusion protein, eg CusB, and an outer-membrane channel component CusC in a CusABC complex driven by import of protons.	Domain
G [auth F], H [auth I], I [auth C]	PF02321	Outer membrane efflux protein (OEP)	Outer membrane efflux protein	-	Family
J [auth K], K [auth L], O [auth J]	PF00873	AcrB/AcrD/AcrF family (ACR_tran)	AcrB/AcrD/AcrF family	-	Family
L [auth M], M [auth N], N [auth O]	PF10766	Multidrug efflux pump-associated protein AcrZ (AcrZ)	Multidrug efflux pump-associated protein AcrZ	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	Multidrug efflux pump subunit AcrA	alkane transmembrane transporter activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity identical protein binding protein binding binding	response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process organic anion transport response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process organic anion transport carboxylic acid transport lipid localization lipid transport organic acid transport monocarboxylic acid transport fatty acid transport macromolecule localization alkane transport response to antibiotic bile acid and bile salt transport organic hydroxy compound transport	protein-containing complex cellular anatomical structure efflux pump complex transmembrane transporter complex membrane membrane protein complex transporter complex cell periphery plasma membrane cell surface periplasmic side of plasma membrane external side of plasma membrane side of membrane outer membrane-bounded periplasmic space protein-containing complex cellular anatomical structure efflux pump complex transmembrane transporter complex membrane membrane protein complex transporter complex cell periphery plasma membrane cell surface periplasmic side of plasma membrane external side of plasma membrane side of membrane outer membrane-bounded periplasmic space cell envelope periplasmic space
G [auth F], H [auth I], I [auth C]	Outer membrane protein TolC	wide pore channel activity passive transmembrane transporter activity channel activity porin activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity wide pore channel activity passive transmembrane transporter activity channel activity porin activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity monoatomic ion channel activity monoatomic ion transmembrane transporter activity efflux transmembrane transporter activity identical protein binding protein binding binding enterobactin transmembrane transporter activity siderophore-iron transmembrane transporter activity	response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process response to antibiotic response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process response to antibiotic monoatomic ion transmembrane transport monoatomic ion transport response to organic cyclic compound organic anion transport carboxylic acid transport lipid localization bile acid and bile salt transport lipid transport organic hydroxy compound transport organic acid transport monocarboxylic acid transport macromolecule localization siderophore transport iron ion transport metal ion transport enterobactin transport transition metal ion transport monoatomic cation transport iron coordination entity transport xenobiotic export from cell xenobiotic detoxification by transmembrane export across the plasma membrane export from cell export across plasma membrane	cellular anatomical structure membrane protein-containing complex efflux pump complex transmembrane transporter complex membrane protein complex transporter complex cell periphery cell surface periplasmic side of plasma membrane plasma membrane external side of plasma membrane side of membrane MacAB-TolC complex cellular anatomical structure membrane protein-containing complex efflux pump complex transmembrane transporter complex membrane protein complex transporter complex cell periphery cell surface periplasmic side of plasma membrane plasma membrane external side of plasma membrane side of membrane MacAB-TolC complex macrolide transmembrane transporter complex outer membrane-bounded periplasmic space cell envelope periplasmic space cell outer membrane external encapsulating structure outer membrane
J [auth K], K [auth L], O [auth J]	Multidrug efflux pump subunit AcrB	alkane transmembrane transporter activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity efflux transmembrane transporter activity xenobiotic transmembrane transporter activity identical protein binding alkane transmembrane transporter activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity efflux transmembrane transporter activity xenobiotic transmembrane transporter activity identical protein binding protein binding binding enterobactin transmembrane transporter activity siderophore-iron transmembrane transporter activity	response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process organic anion transport response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process organic anion transport carboxylic acid transport lipid localization lipid transport organic acid transport monocarboxylic acid transport fatty acid transport macromolecule localization alkane transport response to antibiotic bile acid and bile salt transport organic hydroxy compound transport siderophore transport iron ion transport metal ion transport enterobactin transport transition metal ion transport monoatomic ion transport monoatomic cation transport iron coordination entity transport	cellular anatomical structure membrane protein-containing complex efflux pump complex transmembrane transporter complex membrane protein complex transporter complex cell periphery plasma membrane cell surface periplasmic side of plasma membrane external side of plasma membrane side of membrane
L [auth M], M [auth N], N [auth O]	Multidrug efflux pump accessory protein AcrZ	xenobiotic transmembrane transporter activity transporter activity transmembrane transporter activity	xenobiotic export from cell xenobiotic transport response to stimulus xenobiotic detoxification by transmembrane export across the plasma membrane transport detoxification export from cell export across plasma membrane establishment of localization response to toxic substance transmembrane transport localization response to chemical cellular process xenobiotic export from cell xenobiotic transport response to stimulus xenobiotic detoxification by transmembrane export across the plasma membrane transport detoxification export from cell export across plasma membrane establishment of localization response to toxic substance transmembrane transport localization response to chemical cellular process response to antibiotic	cell periphery cellular anatomical structure plasma membrane membrane cell outer membrane external encapsulating structure cell envelope outer membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	IPR006143	RND efflux pump, membrane fusion protein	Family
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	IPR032317	RND efflux pump, membrane fusion protein, barrel-sandwich domain	Domain
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	IPR051160	Membrane Fusion Protein (MFP) Efflux	Family
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B]	IPR043602	Cation efflux system protein CusB, domain 1	Domain
G [auth F], H [auth I], I [auth C]	IPR051906	Bacterial Outer Membrane Factor (OMF)	Family
G [auth F], H [auth I], I [auth C]	IPR010130	Type I secretion outer membrane protein, TolC	Family
G [auth F], H [auth I], I [auth C]	IPR003423	Outer membrane efflux protein	Family
J [auth K], K [auth L], O [auth J]	IPR027463	Multidrug efflux transporter AcrB TolC docking domain, DN/DC subdomains	Homologous Superfamily
J [auth K], K [auth L], O [auth J]	IPR001036	Acriflavin resistance protein	Family
J [auth K], K [auth L], O [auth J]	IPR004764	Multidrug resistance protein MdtF-like	Family
L [auth M], M [auth N], N [auth O]	IPR053730	Multidrug Efflux Pump Accessory AcrZ	Homologous Superfamily
L [auth M], M [auth N], N [auth O]	IPR019702	Multidrug efflux pump accessory protein AcrZ	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A] A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B], G [auth F], H [auth I], I [auth C], J [auth K], K [auth L], L [auth M], M [auth N], N [auth O], O [auth J]	OPM	Transmembrane	Alpha-helical polytopic	Resistance-nodulation-cell division	Hydrophobe/amphiphile efflux-1 family

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A] A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B], G [auth F], H [auth I], I [auth C], J [auth K], K [auth L], L [auth M], M [auth N], N [auth O], O [auth J]	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A [auth E], B [auth D], C [auth G], D [auth H], E [auth A] A [auth E], B [auth D], C [auth G], D [auth H], E [auth A], F [auth B], G [auth F], H [auth I], I [auth C], J [auth K], K [auth L], L [auth M], M [auth N], N [auth O], O [auth J]	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Multi-Drug Efflux Transporters	AcrABZ-TolC tripartite multi drug efflux pump with bound puromycin (asymmetric structure)