5V5S

multi-drug efflux; membrane transport; RND superfamily; Drug resistance

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
AMR Gene: CARD Annotation
AMR Gene Family: CARD Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Outer membrane efflux proteins (OEP)	8037933	3000246	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Outer membrane efflux proteins (OEP)	8037933	3000246	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Outer membrane efflux proteins (OEP)	8037933	3000246	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042523	3000503	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042528	3000503	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042531	3000526	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042533	3000526	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042533	3000526	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042531	3000526	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042523	3000503	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042528	3000503	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042535	3000769	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042523	3000503	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042531	3000526	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042528	3000503	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB TolC docking domain (DN and DC subdomains)	8042533	3000526	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB transmembrane domain	8042534	3000769	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042525	3000503	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	Multidrug efflux transporter AcrB pore domain PN1, PN2, PC1 and PC2 subdomains	8042529	3000503	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	OEP	e5v5sA1	A: a+b duplicates or obligate multimers	X: Outer membrane efflux proteins (OEP) (From Topology)	H: Outer membrane efflux proteins (OEP) (From Topology)	T: Outer membrane efflux proteins (OEP)	F: OEP	ECOD (1.6)
B	OEP	e5v5sB1	A: a+b duplicates or obligate multimers	X: Outer membrane efflux proteins (OEP) (From Topology)	H: Outer membrane efflux proteins (OEP) (From Topology)	T: Outer membrane efflux proteins (OEP)	F: OEP	ECOD (1.6)
C	OEP	e5v5sC1	A: a+b duplicates or obligate multimers	X: Outer membrane efflux proteins (OEP) (From Topology)	H: Outer membrane efflux proteins (OEP) (From Topology)	T: Outer membrane efflux proteins (OEP)	F: OEP	ECOD (1.6)
D	PRK15030_2nd	e5v5sD1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
D	HlyD_D23_C_1	e5v5sD3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
D	HlyD_2nd	e5v5sD2	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
D	HlyD_1st	e5v5sD4	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
E	PRK15030_2nd	e5v5sE4	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
E	HlyD_D23_C_1	e5v5sE1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
E	HlyD_2nd	e5v5sE2	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
E	HlyD_1st	e5v5sE3	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
F	PRK15030_2nd	e5v5sF3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
F	HlyD_D23_C_1	e5v5sF1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
F	HlyD_2nd	e5v5sF2	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
F	HlyD_1st	e5v5sF4	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
G	PRK15030_2nd	e5v5sG3	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
G	HlyD_D23_C_1	e5v5sG1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
G	HlyD_2nd	e5v5sG4	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
G	HlyD_1st	e5v5sG2	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
H	PRK15030_2nd	e5v5sH2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
H	HlyD_D23_C_1	e5v5sH4	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
H	HlyD_2nd	e5v5sH1	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
H	HlyD_1st	e5v5sH3	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
I	PRK15030_2nd	e5v5sI2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PRK15030_2nd	ECOD (1.6)
I	HlyD_D23_C_1	e5v5sI4	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: HlyD_D23_C_1	ECOD (1.6)
I	HlyD_2nd	e5v5sI3	A: alpha bundles	X: helical hairpin of HlyD-like secretion proteins (From Topology)	H: helical hairpin of HlyD-like secretion proteins (From Topology)	T: helical hairpin of HlyD-like secretion proteins	F: HlyD_2nd	ECOD (1.6)
I	HlyD_1st	e5v5sI1	A: a+b complex topology	X: alpha/beta-Hammerhead/Barrel-sandwich hybrid	H: alpha/beta-Hammerhead/Barrel-sandwich hybrid	T: Single hybrid motif	F: HlyD_1st	ECOD (1.6)
J	ACR_tran_4th	e5v5sJ6	A: alpha bundles	X: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	H: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	T: Multidrug efflux transporter AcrB transmembrane domain	F: ACR_tran_4th	ECOD (1.6)
J	ACR_tran_1st	e5v5sJ5	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_1st	ECOD (1.6)
J	ACR_tran_5th	e5v5sJ3	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_5th	ECOD (1.6)
J	ACR_tran_3rd	e5v5sJ8	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_3rd	ECOD (1.6)
J	ACR_tran_6th	e5v5sJ4	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_6th	ECOD (1.6)
J	ACR_tran_2nd	e5v5sJ1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_2nd	ECOD (1.6)
J	ACR_tran_6th_1	e5v5sJ2	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_6th_1	ECOD (1.6)
K	ACR_tran_4th	e5v5sK1	A: alpha bundles	X: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	H: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	T: Multidrug efflux transporter AcrB transmembrane domain	F: ACR_tran_4th	ECOD (1.6)
K	ACR_tran_1st	e5v5sK4	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_1st	ECOD (1.6)
K	ACR_tran_5th	e5v5sK8	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_5th	ECOD (1.6)
K	ACR_tran_3rd	e5v5sK2	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_3rd	ECOD (1.6)
K	ACR_tran_6th	e5v5sK7	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_6th	ECOD (1.6)
K	ACR_tran_2nd	e5v5sK6	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_2nd	ECOD (1.6)
K	ACR_tran_6th_1	e5v5sK3	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_6th_1	ECOD (1.6)
L	ACR_tran_4th	e5v5sL2	A: alpha bundles	X: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	H: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	T: Multidrug efflux transporter AcrB transmembrane domain	F: ACR_tran_4th	ECOD (1.6)
L	ACR_tran_1st	e5v5sL7	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_1st	ECOD (1.6)
L	ACR_tran_5th	e5v5sL5	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_5th	ECOD (1.6)
L	ACR_tran_3rd	e5v5sL1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_3rd	ECOD (1.6)
L	ACR_tran_6th	e5v5sL6	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: ACR_tran_6th	ECOD (1.6)
L	ACR_tran_2nd	e5v5sL3	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_2nd	ECOD (1.6)
L	ACR_tran_6th_1	e5v5sL4	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB TolC docking domain	F: ACR_tran_6th_1	ECOD (1.6)

AMR Gene: CARD Annotation CARD Database Homepage

Chains	Accession	Short Name	Description	Provenance Source (Version)
J, K, L	ARO:3000216	acrB	Protein subunit of AcrA-AcrB-TolC multidrug efflux complex. AcrB functions as a herterotrimer which forms the inner membrane component and is primarily responsible for substrate recognition and energy transduction by acting as a drug/proton antiporter.	CARD (3.3.0)

AMR Gene Family: CARD Annotation CARD Database Homepage

Chains	Accession	Gene Family	Drug Class	Resistance Machanism	Provenance Source (Version)
D, E, F, G, H D, E, F, G, H, I	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
D, E, F, G, H D, E, F, G, H, I	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
J, K, L	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)
J, K, L	ARO:0010004	resistance-nodulation-cell division (RND) antibiotic efflux pump	fluoroquinolone antibiotic, cephalosporin, glycylcycline, penam, tetracycline antibiotic, rifamycin antibiotic, phenicol antibiotic, disinfecting agents and antiseptics	antibiotic efflux	CARD (3.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF02321	Outer membrane efflux protein (OEP)	Outer membrane efflux protein	-	Family
D, E, F, G, H D, E, F, G, H, I	PF16576	Barrel-sandwich domain of CusB or HlyD membrane-fusion (HlyD_D23)	Barrel-sandwich domain of CusB or HlyD membrane-fusion	HlyD_D23 is the combined domains 2 and 3 of the membrane-fusion proteins CusB and HlyD, which forms a barrel-sandwich. CusB and HlyD proteins are membrane fusion proteins of the CusCFBA copper efflux system in E.coli and related bacteria. The whole ...	HlyD_D23 is the combined domains 2 and 3 of the membrane-fusion proteins CusB and HlyD, which forms a barrel-sandwich. CusB and HlyD proteins are membrane fusion proteins of the CusCFBA copper efflux system in E.coli and related bacteria. The whole molecule hinges between D2 and D3. Efflux systems of this resistance-nodulation-division group - RND - have been developed to excrete poisonous metal ions, and in E.coli the only one that deals with silver and copper is the CusA transporter. The transporter CusA works in conjunction with a periplasmic component that is a membrane fusion protein, eg CusB, and an outer-membrane channel component CusC in a CusABC complex driven by import of protons.	Domain
J, K, L	PF00873	AcrB/AcrD/AcrF family (ACR_tran)	AcrB/AcrD/AcrF family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	Outer membrane protein TolC	wide pore channel activity passive transmembrane transporter activity channel activity porin activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity wide pore channel activity passive transmembrane transporter activity channel activity porin activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity monoatomic ion channel activity monoatomic ion transmembrane transporter activity efflux transmembrane transporter activity identical protein binding protein binding binding enterobactin transmembrane transporter activity siderophore-iron transmembrane transporter activity	response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process response to antibiotic response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process response to antibiotic monoatomic ion transmembrane transport monoatomic ion transport response to organic cyclic compound organic anion transport carboxylic acid transport lipid localization bile acid and bile salt transport lipid transport organic hydroxy compound transport organic acid transport monocarboxylic acid transport macromolecule localization siderophore transport iron ion transport metal ion transport enterobactin transport transition metal ion transport monoatomic cation transport iron coordination entity transport xenobiotic export from cell xenobiotic detoxification by transmembrane export across the plasma membrane export from cell export across plasma membrane	cellular anatomical structure membrane protein-containing complex efflux pump complex transmembrane transporter complex membrane protein complex transporter complex cell periphery cell surface periplasmic side of plasma membrane plasma membrane external side of plasma membrane side of membrane MacAB-TolC complex cellular anatomical structure membrane protein-containing complex efflux pump complex transmembrane transporter complex membrane protein complex transporter complex cell periphery cell surface periplasmic side of plasma membrane plasma membrane external side of plasma membrane side of membrane MacAB-TolC complex macrolide transmembrane transporter complex outer membrane-bounded periplasmic space cell envelope periplasmic space cell outer membrane external encapsulating structure outer membrane
D, E, F, G, H D, E, F, G, H, I	Multidrug efflux pump subunit AcrA	alkane transmembrane transporter activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity identical protein binding protein binding binding	response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process organic anion transport response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process organic anion transport carboxylic acid transport lipid localization lipid transport organic acid transport monocarboxylic acid transport fatty acid transport macromolecule localization alkane transport response to antibiotic bile acid and bile salt transport organic hydroxy compound transport	protein-containing complex cellular anatomical structure efflux pump complex transmembrane transporter complex membrane membrane protein complex transporter complex cell periphery plasma membrane cell surface periplasmic side of plasma membrane external side of plasma membrane side of membrane outer membrane-bounded periplasmic space protein-containing complex cellular anatomical structure efflux pump complex transmembrane transporter complex membrane membrane protein complex transporter complex cell periphery plasma membrane cell surface periplasmic side of plasma membrane external side of plasma membrane side of membrane outer membrane-bounded periplasmic space cell envelope periplasmic space
J, K, L	Multidrug efflux pump subunit AcrB	alkane transmembrane transporter activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity efflux transmembrane transporter activity xenobiotic transmembrane transporter activity identical protein binding alkane transmembrane transporter activity transporter activity transmembrane transporter activity lipid transporter activity lipid transmembrane transporter activity organic hydroxy compound transmembrane transporter activity monocarboxylic acid transmembrane transporter activity carboxylic acid transmembrane transporter activity organic acid transmembrane transporter activity organic anion transmembrane transporter activity bile acid transmembrane transporter activity efflux transmembrane transporter activity xenobiotic transmembrane transporter activity identical protein binding protein binding binding enterobactin transmembrane transporter activity siderophore-iron transmembrane transporter activity	response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process organic anion transport response to stimulus response to chemical response to xenobiotic stimulus xenobiotic transport transport detoxification export across cell outer membrane xenobiotic detoxification by transmembrane export across the cell outer membrane establishment of localization response to toxic substance transmembrane transport localization cellular process organic anion transport carboxylic acid transport lipid localization lipid transport organic acid transport monocarboxylic acid transport fatty acid transport macromolecule localization alkane transport response to antibiotic bile acid and bile salt transport organic hydroxy compound transport siderophore transport iron ion transport metal ion transport enterobactin transport transition metal ion transport monoatomic ion transport monoatomic cation transport iron coordination entity transport	cellular anatomical structure membrane protein-containing complex efflux pump complex transmembrane transporter complex membrane protein complex transporter complex cell periphery plasma membrane cell surface periplasmic side of plasma membrane external side of plasma membrane side of membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR051906	Bacterial Outer Membrane Factor (OMF)	Family
A, B, C	IPR010130	Type I secretion outer membrane protein, TolC	Family
A, B, C	IPR003423	Outer membrane efflux protein	Family
D, E, F, G, H D, E, F, G, H, I	IPR006143	RND efflux pump, membrane fusion protein	Family
D, E, F, G, H D, E, F, G, H, I	IPR032317	RND efflux pump, membrane fusion protein, barrel-sandwich domain	Domain
D, E, F, G, H D, E, F, G, H, I	IPR051160	Membrane Fusion Protein (MFP) Efflux	Family
D, E, F, G, H D, E, F, G, H, I	IPR043602	Cation efflux system protein CusB, domain 1	Domain
J, K, L	IPR027463	Multidrug efflux transporter AcrB TolC docking domain, DN/DC subdomains	Homologous Superfamily
J, K, L	IPR001036	Acriflavin resistance protein	Family
J, K, L	IPR004764	Multidrug resistance protein MdtF-like	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	OPM	Transmembrane	Alpha-helical polytopic	Resistance-nodulation-cell division	Hydrophobe/amphiphile efflux-1 family

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Multi-Drug Efflux Transporters	AcrABZ-TolC tripartite multi drug efflux pump with bound puromycin (asymmetric structure)