6N9O

Crystal structure of human GSDMD

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Gasdermin_N	e6n9oA1	A: beta complex topology	X: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains (From Topology)	H: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains (From Topology)	T: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains	F: Gasdermin_N	ECOD (1.6)
A	Gasdermin_C	e6n9oA2	A: a+b two layers	X: R3H domain-like	H: PUB domain (From Topology)	T: PUB domain	F: Gasdermin_C	ECOD (1.6)
B	Gasdermin_N	e6n9oB2	A: beta complex topology	X: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains (From Topology)	H: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains (From Topology)	T: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains	F: Gasdermin_N	ECOD (1.6)
B	Gasdermin_C	e6n9oB1	A: a+b two layers	X: R3H domain-like	H: PUB domain (From Topology)	T: PUB domain	F: Gasdermin_C	ECOD (1.6)
C	Gasdermin_N	e6n9oC2	A: beta complex topology	X: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains (From Topology)	H: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains (From Topology)	T: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains	F: Gasdermin_N	ECOD (1.6)
C	Gasdermin_C	e6n9oC1	A: a+b two layers	X: R3H domain-like	H: PUB domain (From Topology)	T: PUB domain	F: Gasdermin_C	ECOD (1.6)
D	Gasdermin_N	e6n9oD2	A: beta complex topology	X: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains (From Topology)	H: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains (From Topology)	T: Membrane attack complex/perforin (MACPF) and cholesterol-dependent cytolysin (CDC) domains	F: Gasdermin_N	ECOD (1.6)
D	Gasdermin_C	e6n9oD1	A: a+b two layers	X: R3H domain-like	H: PUB domain (From Topology)	T: PUB domain	F: Gasdermin_C	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF17708	Gasdermin PUB domain (Gasdermin_C)	Gasdermin PUB domain	The precise function of this protein is unknown. A deletion/insertion mutation is associated with an autosomal dominant non-syndromic hearing impairment form [1]. In addition, this protein has also been found to contribute to acquired etoposide resis ...	The precise function of this protein is unknown. A deletion/insertion mutation is associated with an autosomal dominant non-syndromic hearing impairment form [1]. In addition, this protein has also been found to contribute to acquired etoposide resistance in melanoma cells [2]. This family also includes the gasdermin protein [3]	Domain
A, B, C, D	PF04598	Gasdermin pore forming domain (Gasdermin)	Gasdermin pore forming domain	The precise function of this protein is unknown. A deletion/insertion mutation is associated with an autosomal dominant non-syndromic hearing impairment form [1]. In addition, this protein has also been found to contribute to acquired etoposide resis ...	The precise function of this protein is unknown. A deletion/insertion mutation is associated with an autosomal dominant non-syndromic hearing impairment form [1]. In addition, this protein has also been found to contribute to acquired etoposide resistance in melanoma cells [2]. This family also includes the gasdermin protein [3]	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Gasdermin-D	wide pore channel activity passive transmembrane transporter activity channel activity transporter activity transmembrane transporter activity ion binding binding anion binding phosphatidic acid binding phospholipid binding lipid binding small molecule binding phosphatidylglycerol binding cardiolipin binding wide pore channel activity passive transmembrane transporter activity channel activity transporter activity transmembrane transporter activity ion binding binding anion binding phosphatidic acid binding phospholipid binding lipid binding small molecule binding phosphatidylglycerol binding cardiolipin binding phosphatidylinositol-4,5-bisphosphate binding phosphatidylinositol bisphosphate binding phosphatidylinositol phosphate binding phosphatidylinositol binding phosphatidylinositol-4-phosphate binding modified amino acid binding phosphatidylserine binding	regulation of defense response regulation of biological process response to stimulus regulation of response to external stimulus defense response biological regulation positive regulation of biological process response to external stimulus regulation of inflammatory response positive regulation of response to stimulus positive regulation of response to external stimulus positive regulation of inflammatory response regulation of response to stimulus regulation of response to stress response to stress regulation of defense response regulation of biological process response to stimulus regulation of response to external stimulus defense response biological regulation positive regulation of biological process response to external stimulus regulation of inflammatory response positive regulation of response to stimulus positive regulation of response to external stimulus positive regulation of inflammatory response regulation of response to stimulus regulation of response to stress response to stress positive regulation of defense response inflammatory response biological process involved in interspecies interaction between organisms immune response response to other organism defense response to symbiont response to biotic stimulus immune system process defense response to other organism innate immune response response to external biotic stimulus cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis pore complex assembly defense response to bacterium response to bacterium multicellular organismal process regulation of cellular process interleukin-1 production regulation of interleukin-1 beta production positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of interleukin-1 beta production positive regulation of biosynthetic process regulation of cytokine production cytokine production regulation of multicellular organismal process positive regulation of interleukin-1 production positive regulation of macromolecule metabolic process positive regulation of cellular process regulation of biosynthetic process regulation of interleukin-1 production regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of gene expression biosynthetic process interleukin-1 beta production positive regulation of gene expression positive regulation of multicellular organismal process regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process positive regulation of cytokine production regulation of metabolic process pyroptotic cell death pyroptotic inflammatory response cell death programmed cell death protein localization establishment of protein localization cellular localization nitrogen compound transport transport secretion establishment of protein localization to extracellular region export from cell protein secretion establishment of localization protein localization to extracellular region localization secretion by cell protein transport cellular macromolecule localization macromolecule localization defense response to Gram-negative bacterium protein complex oligomerization protein homooligomerization defense response to Gram-positive bacterium	cellular anatomical structure membrane membrane-bounded organelle mitochondrial membrane intracellular anatomical structure mitochondrion intracellular membrane-bounded organelle intracellular organelle organelle envelope mitochondrial envelope organelle cytoplasm organelle membrane cytosol cellular anatomical structure membrane membrane-bounded organelle mitochondrial membrane intracellular anatomical structure mitochondrion intracellular membrane-bounded organelle intracellular organelle organelle envelope mitochondrial envelope organelle cytoplasm organelle membrane cytosol cell periphery plasma membrane membrane-enclosed lumen intracellular vesicle ficolin-1-rich granule endomembrane system intracellular organelle lumen organelle lumen cytoplasmic vesicle ficolin-1-rich granule lumen secretory granule secretory vesicle vesicle nucleus nucleoplasm nuclear lumen tertiary granule lumen tertiary granule extracellular region canonical inflammasome complex protein-containing complex NLRP3 inflammasome complex vesicle lumen cytoplasmic vesicle lumen secretory granule lumen specific granule lumen specific granule extracellular space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR040460	Gasdermin, pore forming domain	Domain
A, B, C, D	IPR041263	Gasdermin, PUB domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: P57764	Alcoholism ovarian cancer tuberculosis cutaneous lupus erythematosus pituitary cancer osteosarcoma psoriasis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.