ELECTRON MICROSCOPY
Refinement
| RMS Deviations | |
|---|---|
| Key | Refinement Restraint Deviation |
| r_dihedral_angle_3_deg | 10.602 |
| r_long_range_B_other | 8.322 |
| r_long_range_B_refined | 8.321 |
| r_dihedral_angle_1_deg | 5.127 |
| r_dihedral_angle_2_deg | 3.849 |
| r_mcangle_it | 2.851 |
| r_mcangle_other | 2.85 |
| r_scangle_other | 2.061 |
| r_mcbond_it | 1.536 |
| r_mcbond_other | 1.535 |
| Sample |
|---|
| Complex of apo human mitochondrial pyruvate carrier (MPC1L/MPC2) with a pro-macrobody |
| Sample Components |
| Mitochondrial pyruvate carrier (MPC1L/MPC2) |
| Pro-macrobody (fusion of a nanobody with Maltose-binding protein) |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK IV |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 164876 |
| Reported Resolution (?) | 3.65 |
| Resolution Method | FSC 0.143 CUT-OFF |
| Other Details | |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C1 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | FLEXIBLE FIT | ||||
| Refinement Target | Cross-correlation coefficient | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | |||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | TFS FALCON 4i (4k x 4k) | ||||||||
| Electron Dose (electrons/?**2) | 54.44 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | |
| Temperature (Kelvin) | |
| Microscope Model | FEI TITAN KRIOS |
| Minimum Defocus (nm) | 600 |
| Maximum Defocus (nm) | 1800 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | FEI TITAN KRIOS AUTOGRID HOLDER |
| Nominal Magnification | 165000 |
| Calibrated Magnification | |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| PARTICLE SELECTION | Warp | |
| IMAGE ACQUISITION | EPU | |
| CTF CORRECTION | Warp | |
| MODEL FITTING | Coot | 0.9.8.92 |
| MODEL FITTING | ISOLDE | |
| MODEL FITTING | UCSF ChimeraX | 1.7.1 |
| INITIAL EULER ASSIGNMENT | cryoSPARC | 3.3.2 |
| FINAL EULER ASSIGNMENT | cryoSPARC | 3.3.2 |
| CLASSIFICATION | cryoSPARC | 3.3.2 |
| RECONSTRUCTION | cryoSPARC | 3.3.2 |
| MODEL REFINEMENT | REFMAC | 5.8.0419 |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| PHASE FLIPPING AND AMPLITUDE CORRECTION | 1329798 | |||
