Download MendeleyEnzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis
Garrido-Franco, M., Laber, B., Huber, R., Clausen, T.(2002) J Mol Biol 321: 601-612
- PubMed: 12206776
- DOI: https://doi.org/10.1016/s0022-2836(02)00695-2
- Primary Citation of Related Structures:
1IXN, 1IXO, 1IXP, 1IXQ - PubMed Abstract:
Pyridoxine 5'-phosphate (PNP) synthase is the last enzyme in the de novo biosynthesis of vitamin B(6) catalyzing the complicated ring-closure reaction between 1-deoxy-D-xylulose-5-phosphate and 1-amino-acetone-3-phosphate. Here we present the crystal structures of four PNP synthase complexes with substrates and substrate analogs. While the overall fold of the enzyme is conserved in all complexes, characteristic readjustments were observed in the active site. The complementary structural information allowed us to postulate a detailed reaction mechanism. The observed binding mode of substrates indicates how the first reaction intermediate, the Schiff-base conjugate, is formed. The most important mechanistic features are the presence of two phosphate-binding sites with distinct affinities and the existence of a water relay system for the release of reaction water molecules. Furthermore, the complexes provide the basis to rationalize the open-closed transition of a flexible loop located on the C-terminal side of the TIM-barrel. Binding of both substrate molecules to the active site seems to be a prerequisite to trigger this transition. Highly conserved mechanistically important residues in the PNP synthase family imply a similar active site organization and reaction mechanism for all family members. Due to the exclusive presence of PNP synthase in a subset of eubacteria, including several well-known pathogens, and due to its outstanding physiological importance for these organisms, the enzyme appears to be a promising novel target for antibacterial drug design.
Organizational Affiliation:
Max-Planck-Institut f¨¹r Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, Planegg-Martinsried, Germany.
