Download MendeleyTransition state of phospho-enzyme hydrolysis in beta-phosphoglucomutase.
Robertson, A.J., Bisson, C., Waltho, J.P.To be published.
6HDI
R49A variant of beta-phosphoglucomutase from Lactococcus lactis in an open conformer to 2.0 A.
- PDB DOI: https://doi.org/10.2210/pdb6HDI/pdb
- Classification: ISOMERASE
- Organism(s): Lactococcus lactis subsp. lactis Il1403
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2018-08-17 Released: 2020-08-26
- Funding Organization(s): Biotechnology and Biological Sciences Research Council
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.03 ?
- R-Value Free: 0.273
- R-Value Work: 0.205
- R-Value Observed: 0.209
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Beta-phosphoglucomutase | 221 | Lactococcus lactis subsp. lactis Il1403 | Mutation(s): 3 Gene Names: pgmB, LL0429, L0001 EC: 5.4.2.6 |  | |
UniProt | |||||
Find proteins for P71447 (Lactococcus lactis subsp. lactis (strain IL1403)) Explore P71447 Go to UniProtKB: P71447 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P71447 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| EDO Query on EDO | D [auth A], E [auth A], F [auth A], H [auth B] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
| MG Query on MG | C [auth A], G [auth B] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.03 ?
- R-Value Free: 0.273
- R-Value Work: 0.205
- R-Value Observed: 0.209
- Space Group: P 1 21 1
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 38.2 | ¦Á = 90 |
| b = 116.9 | ¦Â = 98.09 |
| c = 53.17 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| xia2 | data reduction |
| xia2 | data scaling |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2020-08-26 Deposition Author(s): Robertson, A.J., Bisson, C., Waltho, J.P.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Biotechnology and Biological Sciences Research Council | United Kingdom | BB/M021637/1 |
| Biotechnology and Biological Sciences Research Council | United Kingdom | BB/K016245/1 |
Revision History (Full details and data files)
- Version 1.0: 2020-08-26
Type: Initial release - Version 1.1: 2024-01-17
Changes: Data collection, Database references, Refinement description
