8S83

Domains 3 and 6 of Sap S-layer protein from Bacillus anthracis

PDB DOI: https://doi.org/10.2210/pdb8S83/pdb

Classification: STRUCTURAL PROTEIN
Organism(s): Bacillus anthracis
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2024-03-05 Released: 2024-11-20
Deposition Author(s): Sogues, A., Remaut, H.
Funding Organization(s): European Molecular Biology Organization (EMBO)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.07 ?
R-Value Free: 0.258
R-Value Work: 0.201
R-Value Observed: 0.204

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Architecture of the Sap S-layer of Bacillus anthracis revealed by integrative structural biology

Sogues, A., Remaut, H.

To be published.

Macromolecule Content

Total Structure Weight: 47.59 kDa
Atom Count: 3,424
Modelled Residue Count: 419
Deposited Residue Count: 444
Unique protein chains: 2

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
S-layer protein sap	A, B	113	Bacillus anthracis	Mutation(s): 0 Gene Names: sap, BA_0885, GBAA_0885, BAS0841
UniProt
Find proteins for P49051 (Bacillus anthracis) Explore P49051 Go to UniProtKB: P49051
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P49051
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
S-layer protein sap	C, D	109	Bacillus anthracis	Mutation(s): 0 Gene Names: sap, BA_0885, GBAA_0885, BAS0841
UniProt
Find proteins for P49051 (Bacillus anthracis) Explore P49051 Go to UniProtKB: P49051
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P49051
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.07 ?
R-Value Free: 0.258
R-Value Work: 0.201
R-Value Observed: 0.204
Space Group: P 1 2₁ 1

Unit Cell:

Length ( ? )	Angle ( ? )
a = 40.154	α = 90
b = 61.01	β = 91.5
c = 97.658	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
XDS	data reduction
Aimless	data scaling
PHASER	phasing

Structure Validation

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Entry History & Funding Information

Deposition Data

Released Date: 2024-11-20

Deposition Author(s):

Funding Organization	Location	Grant Number
European Molecular Biology Organization (EMBO)	European Union	ALTF 709-2021

Revision History (Full details and data files)

Version 1.0: 2024-11-20
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.