9GUV

30S mRNA delivery complex (closed-head)

PDB DOI: https://doi.org/10.2210/pdb9GUV/pdb
EM Map EMD-51621: EMDB EMDataResource

Classification: RIBOSOME
Organism(s): Escherichia coli K-12, synthetic construct
Expression System: Escherichia coli K-12
Mutation(s): No

Deposited: 2024-09-20 Released: 2024-11-20
Deposition Author(s): Rahil, H., Webster, M.W., Weixlbaumer, A.
Funding Organization(s): European Research Council (ERC), Agence Nationale de la Recherche (ANR), European Molecular Biology Organization (EMBO), National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Wellcome Trust

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 3.00 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Molecular basis of mRNA delivery to the bacterial ribosome

Webster, M.W., Chauvier, A., Rahil, H., Graziadei, A., Miropolskaya, N., Charles, K., Takacs, M., Saintandre, C., Rappsilber, J., Walter, N.G., Weixlbaumer, A.

(2024) Science

DOI: https://doi.org/10.1126/science.ado8476

Macromolecule Content

Total Structure Weight: 891.5 kDa
Atom Count: 55,998
Modelled Residue Count: 4,407
Deposited Residue Count: 4,775
Unique protein chains: 21
Unique nucleic acid chains: 3

Macromolecules

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Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S1	B	557	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsA, ssyF, b0911, JW0894
UniProt
Find proteins for P0AG67 (Escherichia coli (strain K12)) Explore P0AG67 Go to UniProtKB: P0AG67
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UniProt Group	P0AG67
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Reference Sequence

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Entity ID: 3
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S2	C	241	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsB, b0169, JW0164
UniProt
Find proteins for P0A7V0 (Escherichia coli (strain K12)) Explore P0A7V0 Go to UniProtKB: P0A7V0
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UniProt Group	P0A7V0
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Reference Sequence

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Entity ID: 4
Molecule	Chains	Sequence Length	Organism	Details	Image
Small ribosomal subunit protein uS3	D	233	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsC, A5U30_004548, A8502_004027, ACN81_25500, ACU57_20980, AW118_24880, AWP47_12665, B6R15_004073, B6R31_004972, BANRA_01945... rpsC, A5U30_004548, A8502_004027, ACN81_25500, ACU57_20980, AW118_24880, AWP47_12665, B6R15_004073, B6R31_004972, BANRA_01945, BANRA_04324, BCB93_004289, BE932_15515, BER14_25170, BG944_001129, BGM66_003597, BGZ_02594, BGZ_04121, BJI68_08695, BK292_20485, BK383_24665, BKL28_004547, BR158_003786, BTB68_004360, BTQ06_10670, BvCmsKKP061_03026, BvCmsSIP010_02681, BXT93_05495, C0P57_002664, C1Q91_004908, C2121_004166, C2M16_25425, C2R31_004629, C3F40_19740, C9Z68_22615, CF22_004853, CG704_20890, CIG67_10695, CQ842_14345, CQ842_22325, CTR35_003626, CV83915_02784, D3C88_13310, D3G36_23070, D4M65_20635, D4N09_20235, D9D43_22570, D9E49_05220, D9J61_16715, DD762_23610, DIV22_15195, DNQ45_03820, DNX30_25415, DS732_24325, DTL43_20935, DU321_12065, E2865_04467, E4K51_21555, E5H86_24460, E6D34_21695, EAI46_07540, ECs4179, EIA08_24020, EIZ93_12405, EN85_004353, EPS76_07150, EPS97_20030, EWK56_25060, ExPECSC038_03644, F7F11_22660, F7N46_23945, F9413_21170, F9461_25590, F9B07_24620, FGAF848_25890, FIJ20_21130, FJQ40_18370, FKO60_25700, FOI11_019195, FOI11_03985, FPI65_20370, FPS11_25365, FVB16_04935, FWK02_12630, FZU14_21885, G3V95_19910, G3W53_21020, G4A38_21575, G4A47_20845, G5603_24650, GAI89_24765, GAJ12_24655, GFY48_21635, GGB84_004455, GKF66_21610, GNW61_16695, GNZ05_25920, GOP25_22770, GP711_23370, GP954_00880, GP965_04360, GP975_01250, GP979_01940, GQA06_03885, GQE86_20145, GQM04_10630, GQM13_25265, GQM21_11295, GQN34_23330, GQW07_21485, GRO95_20750, GRW05_09125, GRW24_04690, GUC01_21245, H0O53_20765, H0O72_19480, HEP30_018700, HEP34_004758, HHH44_004523, HI055_004152, HIE29_005199, HJQ60_004999, HLX92_10010, HLZ50_22115, HMV95_19670, HMW38_23170, HV109_02125, HV209_14650, HVV39_09330, HVW04_17470, HVW43_18605, HVY77_02110, I6H00_20860, I6H02_12055, J0541_004409, J5U05_004075, JNP96_25370, NCTC10082_02827, NCTC10089_00490, NCTC10429_01082, NCTC10764_03851, NCTC10767_01504, NCTC10974_00537, NCTC11126_02758, NCTC11181_02726, NCTC11341_01901, NCTC7922_04054, NCTC7927_00539, NCTC7928_02529, NCTC8009_01498, NCTC8179_05929, NCTC8333_00477, NCTC8500_00322, NCTC8621_00496, NCTC8622_00518, NCTC8959_03273, NCTC8960_03058, NCTC9001_04242, NCTC9045_00553, NCTC9075_00712, NCTC9077_00592, NCTC9081_00905, NCTC9117_00744, NCTC9702_00525, NCTC9706_02725, NCTC9962_06785, OGM49_22395, P6223_003995, QDW62_02130, RZR61_12955, SAMEA3472112_00681, SAMEA3752557_01964, WR15_19670
UniProt
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Reference Sequence

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Entity ID: 5
Molecule	Chains	Sequence Length	Organism	Details	Image
Small ribosomal subunit protein uS4	E	206	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsD, BWG_2987
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UniProt Group	C4ZUF1
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Reference Sequence

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Entity ID: 6
Molecule	Chains	Sequence Length	Organism	Details	Image
Small ribosomal subunit protein uS5	F	165	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsE, A5U30_004537, ACN81_25445, ACU57_21035, AW118_24825, AWP47_12720, B6R15_004084, B6R31_004983, BANRA_01934, BANRA_04313... rpsE, A5U30_004537, ACN81_25445, ACU57_21035, AW118_24825, AWP47_12720, B6R15_004084, B6R31_004983, BANRA_01934, BANRA_04313, BCB93_004278, BE932_15460, BER14_25115, BG944_001118, BGM66_003608, BGZ_02605, BGZ_04110, BJI68_08750, BK292_20430, BK383_24720, BKL28_004558, BR158_003775, BTB68_004371, BTQ06_10615, BvCmsKKP061_03015, BvCmsSIP010_02670, BXT93_05550, C0P57_002653, C1Q91_004919, C2121_004177, C2M16_25480, C2R31_004618, C3F40_19795, C9Z68_22560, CF22_004864, CG704_20945, CIG67_10750, CQ842_14400, CQ842_22270, CTR35_003615, CV83915_02795, D3C88_28455, D3G36_23125, D4M65_20580, D4N09_20180, D9D43_22625, D9E49_05165, D9J61_16660, DD762_23555, DIV22_15250, DNQ45_03765, DNX30_25470, DS732_24270, DTL43_20990, DU321_12010, E2865_04456, E4K51_21500, E5H86_24515, E6D34_21750, EAI46_07485, ECs4168, EIA08_23965, EIZ93_12460, EN85_004364, EPS76_07205, EPS97_20085, EWK56_25005, ExPECSC038_03655, F7F11_22715, F7N46_24000, F9413_21225, F9461_25645, F9B07_24675, FGAF848_26000, FIJ20_21185, FJQ40_18315, FKO60_25645, FOI11_019250, FOI11_03930, FPI65_20315, FPS11_25420, FVB16_04880, FWK02_06280, FZU14_21830, G3V95_19855, G3W53_20965, G4A38_21520, G4A47_20790, G5603_24595, GAI89_24820, GAJ12_24710, GFY48_21580, GGB84_004466, GKF66_21555, GNW61_16640, GNZ05_25975, GOP25_22715, GP711_23315, GP954_00935, GP965_04305, GP975_01195, GP979_01995, GQA06_03940, GQE86_20090, GQM04_10575, GQM13_25210, GQM21_11240, GQN34_23275, GQW07_21540, GRO95_20805, GRW05_09070, GRW24_04745, GUC01_21190, H0O72_19425, HEP30_018645, HEP34_004769, HHH44_004534, HI055_004141, HIE29_005188, HJQ60_005010, HLX92_10065, HLZ50_22170, HMV95_19615, HMW38_23115, HV109_02180, HV209_14705, HVV39_09275, HVW04_17525, HVW43_18660, HVY77_02165, I6H00_20915, I6H02_12000, J0541_004398, J5U05_004086, JNP96_25315, NCTC10082_02838, NCTC10089_00501, NCTC10418_00689, NCTC10429_01094, NCTC10764_03862, NCTC10767_01515, NCTC10865_00660, NCTC11126_02770, NCTC11181_02737, NCTC11341_01890, NCTC7922_04043, NCTC7927_00550, NCTC8179_05941, NCTC8333_00488, NCTC8621_00507, NCTC8622_00508, NCTC8959_03262, NCTC8960_03069, NCTC9001_04253, NCTC9045_00565, NCTC9075_00723, NCTC9077_00603, NCTC9702_00536, NCTC9706_02736, NCTC9962_06796, OGM49_22340, P6223_003984, QDW62_02185, RZR61_12900, SAMEA3472112_00692, SAMEA3752557_01953, WR15_19615
UniProt
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Reference Sequence

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Entity ID: 7
Molecule	Chains	Sequence Length	Organism	Details	Image
Small ribosomal subunit protein bS6	G	131	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsF, BANRA_02890, BGM66_001638, BJI68_10200, C0P57_000210, CIG67_23060, CTR35_001516, CV83915_01828, E6D34_03615, G4A38_03220... rpsF, BANRA_02890, BGM66_001638, BJI68_10200, C0P57_000210, CIG67_23060, CTR35_001516, CV83915_01828, E6D34_03615, G4A38_03220, G4A47_10770, GNW61_00195, GQM21_23655, GRW05_28450, HVY77_22975, J0541_002297, JNP96_03175, NCTC8500_05020, NCTC9045_05184, NCTC9706_01764, P6223_000651, SAMEA3752557_01699
UniProt
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Entity ID: 8
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S7	H	156	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsG, b3341, JW3303
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Find proteins for P02359 (Escherichia coli (strain K12)) Explore P02359 Go to UniProtKB: P02359
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UniProt Group	P02359
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Entity ID: 9
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S8	I	130	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsH, b3306, JW3268
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Find proteins for P0A7W7 (Escherichia coli (strain K12)) Explore P0A7W7 Go to UniProtKB: P0A7W7
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UniProt Group	P0A7W7
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Entity ID: 10
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S9	J	130	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsI, b3230, JW3199
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Entity ID: 11
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S10	K	103	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsJ, nusE, b3321, JW3283
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Find proteins for P0A7R5 (Escherichia coli (strain K12)) Explore P0A7R5 Go to UniProtKB: P0A7R5
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Entity ID: 12
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S11	L	129	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsK, b3297, JW3259
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Find proteins for P0A7R9 (Escherichia coli (strain K12)) Explore P0A7R9 Go to UniProtKB: P0A7R9
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UniProt Group	P0A7R9
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Entity ID: 13
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S12	M	124	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsL, strA, b3342, JW3304
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Entity ID: 14
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S13	N	118	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsM, b3298, JW3260
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UniProt Group	P0A7S9
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Entity ID: 15
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S14	O	101	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsN, b3307, JW3269
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Entity ID: 16
Molecule	Chains	Sequence Length	Organism	Details	Image
Small ribosomal subunit protein uS15	P	89	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsO, secC, b3165, JW3134
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Entity ID: 17
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S16	Q	82	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsP, b2609, JW2590
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Find proteins for P0A7T3 (Escherichia coli (strain K12)) Explore P0A7T3 Go to UniProtKB: P0A7T3
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Entity ID: 18
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S17	R	84	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsQ, neaA, b3311, JW3273
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Entity ID: 19
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S18	S	75	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsR, b4202, JW4160
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Entity ID: 20
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S19	T	92	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsS, b3316, JW3278
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Entity ID: 21
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S20	U	87	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsT, b0023, JW0022
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Entity ID: 22
Molecule	Chains	Sequence Length	Organism	Details	Image
30S ribosomal protein S21	V	71	Escherichia coli K-12	Mutation(s): 0 Gene Names: rpsU, b3065, JW3037
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Entity ID: 1
Molecule	Chains	Length	Organism	Image
16S ribosomal RNA	A	1,541	Escherichia coli K-12
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Entity ID: 23
Molecule	Chains	Length	Organism	Image
tRNA(fmet) P-site	W	77	Escherichia coli K-12
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Entity ID: 24
Molecule	Chains	Length	Organism	Image
mRNA	X	53	synthetic construct
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Reference Sequence

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
MG Query on MG Download Ideal Coordinates CCD File SDF format, chain AA [auth A] SDF format, chain AB [auth A] SDF format, chain AC [auth A] SDF format, chain AD [auth A] SDF format, chain BA [auth A] SDF format, chain BB [auth A] SDF format, chain BC [auth A] SDF format, chain BD [auth A] SDF format, chain CA [auth A] SDF format, chain CB [auth A] SDF format, chain CC [auth A] SDF format, chain CD [auth A] SDF format, chain DA [auth A] SDF format, chain DB [auth A] SDF format, chain DC [auth A] SDF format, chain DD [auth A] SDF format, chain EA [auth A] SDF format, chain EB [auth A] SDF format, chain EC [auth A] SDF format, chain ED [auth A] SDF format, chain FA [auth A] SDF format, chain FB [auth A] SDF format, chain FC [auth A] SDF format, chain FD [auth O] SDF format, chain GA [auth A] SDF format, chain GB [auth A] SDF format, chain GC [auth A] SDF format, chain HA [auth A] SDF format, chain HB [auth A] SDF format, chain HC [auth A] SDF format, chain IA [auth A] SDF format, chain IB [auth A] SDF format, chain IC [auth A] SDF format, chain JA [auth A] SDF format, chain JB [auth A] SDF format, chain JC [auth A] SDF format, chain KA [auth A] SDF format, chain KB [auth A] SDF format, chain KC [auth A] SDF format, chain LA [auth A] SDF format, chain LB [auth A] SDF format, chain LC [auth A] SDF format, chain MA [auth A] SDF format, chain MB [auth A] SDF format, chain MC [auth A] SDF format, chain NA [auth A] SDF format, chain NB [auth A] SDF format, chain NC [auth A] SDF format, chain OA [auth A] SDF format, chain OB [auth A] SDF format, chain OC [auth A] SDF format, chain PA [auth A] SDF format, chain PB [auth A] SDF format, chain PC [auth A] SDF format, chain QA [auth A] SDF format, chain QB [auth A] SDF format, chain QC [auth A] SDF format, chain RA [auth A] SDF format, chain RB [auth A] SDF format, chain RC [auth A] SDF format, chain SA [auth A] SDF format, chain SB [auth A] SDF format, chain SC [auth A] SDF format, chain TA [auth A] SDF format, chain TB [auth A] SDF format, chain TC [auth A] SDF format, chain UA [auth A] SDF format, chain UB [auth A] SDF format, chain UC [auth A] SDF format, chain VA [auth A] SDF format, chain VB [auth A] SDF format, chain VC [auth A] SDF format, chain WA [auth A] SDF format, chain WB [auth A] SDF format, chain WC [auth A] SDF format, chain XA [auth A] SDF format, chain XB [auth A] SDF format, chain XC [auth A] SDF format, chain Y [auth A] SDF format, chain YA [auth A] SDF format, chain YB [auth A] SDF format, chain YC [auth A] SDF format, chain Z [auth A] SDF format, chain ZA [auth A] SDF format, chain ZB [auth A] SDF format, chain ZC [auth A] MOL2 format, chain AA [auth A] MOL2 format, chain AB [auth A] MOL2 format, chain AC [auth A] MOL2 format, chain AD [auth A] MOL2 format, chain BA [auth A] MOL2 format, chain BB [auth A] MOL2 format, chain BC [auth A] MOL2 format, chain BD [auth A] MOL2 format, chain CA [auth A] MOL2 format, chain CB [auth A] MOL2 format, chain CC [auth A] MOL2 format, chain CD [auth A] MOL2 format, chain DA [auth A] MOL2 format, chain DB [auth A] MOL2 format, chain DC [auth A] MOL2 format, chain DD [auth A] MOL2 format, chain EA [auth A] MOL2 format, chain EB [auth A] MOL2 format, chain EC [auth A] MOL2 format, chain ED [auth A] MOL2 format, chain FA [auth A] MOL2 format, chain FB [auth A] MOL2 format, chain FC [auth A] MOL2 format, chain FD [auth O] MOL2 format, chain GA [auth A] MOL2 format, chain GB [auth A] MOL2 format, chain GC [auth A] MOL2 format, chain HA [auth A] MOL2 format, chain HB [auth A] MOL2 format, chain HC [auth A] MOL2 format, chain IA [auth A] MOL2 format, chain IB [auth A] MOL2 format, chain IC [auth A] MOL2 format, chain JA [auth A] MOL2 format, chain JB [auth A] MOL2 format, chain JC [auth A] MOL2 format, chain KA [auth A] MOL2 format, chain KB [auth A] MOL2 format, chain KC [auth A] MOL2 format, chain LA [auth A] MOL2 format, chain LB [auth A] MOL2 format, chain LC [auth A] MOL2 format, chain MA [auth A] MOL2 format, chain MB [auth A] MOL2 format, chain MC [auth A] MOL2 format, chain NA [auth A] MOL2 format, chain NB [auth A] MOL2 format, chain NC [auth A] MOL2 format, chain OA [auth A] MOL2 format, chain OB [auth A] MOL2 format, chain OC [auth A] MOL2 format, chain PA [auth A] MOL2 format, chain PB [auth A] MOL2 format, chain PC [auth A] MOL2 format, chain QA [auth A] MOL2 format, chain QB [auth A] MOL2 format, chain QC [auth A] MOL2 format, chain RA [auth A] MOL2 format, chain RB [auth A] MOL2 format, chain RC [auth A] MOL2 format, chain SA [auth A] MOL2 format, chain SB [auth A] MOL2 format, chain SC [auth A] MOL2 format, chain TA [auth A] MOL2 format, chain TB [auth A] MOL2 format, chain TC [auth A] MOL2 format, chain UA [auth A] MOL2 format, chain UB [auth A] MOL2 format, chain UC [auth A] MOL2 format, chain VA [auth A] MOL2 format, chain VB [auth A] MOL2 format, chain VC [auth A] MOL2 format, chain WA [auth A] MOL2 format, chain WB [auth A] MOL2 format, chain WC [auth A] MOL2 format, chain XA [auth A] MOL2 format, chain XB [auth A] MOL2 format, chain XC [auth A] MOL2 format, chain Y [auth A] MOL2 format, chain YA [auth A] MOL2 format, chain YB [auth A] MOL2 format, chain YC [auth A] MOL2 format, chain Z [auth A] MOL2 format, chain ZA [auth A] MOL2 format, chain ZB [auth A] MOL2 format, chain ZC [auth A] mmCIF format, chain AA [auth A] mmCIF format, chain AB [auth A] mmCIF format, chain AC [auth A] mmCIF format, chain AD [auth A] mmCIF format, chain BA [auth A] mmCIF format, chain BB [auth A] mmCIF format, chain BC [auth A] mmCIF format, chain BD [auth A] mmCIF format, chain CA [auth A] mmCIF format, chain CB [auth A] mmCIF format, chain CC [auth A] mmCIF format, chain CD [auth A] mmCIF format, chain DA [auth A] mmCIF format, chain DB [auth A] mmCIF format, chain DC [auth A] mmCIF format, chain DD [auth A] mmCIF format, chain EA [auth A] mmCIF format, chain EB [auth A] mmCIF format, chain EC [auth A] mmCIF format, chain ED [auth A] mmCIF format, chain FA [auth A] mmCIF format, chain FB [auth A] mmCIF format, chain FC [auth A] mmCIF format, chain FD [auth O] mmCIF format, chain GA [auth A] mmCIF format, chain GB [auth A] mmCIF format, chain GC [auth A] mmCIF format, chain HA [auth A] mmCIF format, chain HB [auth A] mmCIF format, chain HC [auth A] mmCIF format, chain IA [auth A] mmCIF format, chain IB [auth A] mmCIF format, chain IC [auth A] mmCIF format, chain JA [auth A] mmCIF format, chain JB [auth A] mmCIF format, chain JC [auth A] mmCIF format, chain KA [auth A] mmCIF format, chain KB [auth A] mmCIF format, chain KC [auth A] mmCIF format, chain LA [auth A] mmCIF format, chain LB [auth A] mmCIF format, chain LC [auth A] mmCIF format, chain MA [auth A] mmCIF format, chain MB [auth A] mmCIF format, chain MC [auth A] mmCIF format, chain NA [auth A] mmCIF format, chain NB [auth A] mmCIF format, chain NC [auth A] mmCIF format, chain OA [auth A] mmCIF format, chain OB [auth A] mmCIF format, chain OC [auth A] mmCIF format, chain PA [auth A] mmCIF format, chain PB [auth A] mmCIF format, chain PC [auth A] mmCIF format, chain QA [auth A] mmCIF format, chain QB [auth A] mmCIF format, chain QC [auth A] mmCIF format, chain RA [auth A] mmCIF format, chain RB [auth A] mmCIF format, chain RC [auth A] mmCIF format, chain SA [auth A] mmCIF format, chain SB [auth A] mmCIF format, chain SC [auth A] mmCIF format, chain TA [auth A] mmCIF format, chain TB [auth A] mmCIF format, chain TC [auth A] mmCIF format, chain UA [auth A] mmCIF format, chain UB [auth A] mmCIF format, chain UC [auth A] mmCIF format, chain VA [auth A] mmCIF format, chain VB [auth A] mmCIF format, chain VC [auth A] mmCIF format, chain WA [auth A] mmCIF format, chain WB [auth A] mmCIF format, chain WC [auth A] mmCIF format, chain XA [auth A] mmCIF format, chain XB [auth A] mmCIF format, chain XC [auth A] mmCIF format, chain Y [auth A] mmCIF format, chain YA [auth A] mmCIF format, chain YB [auth A] mmCIF format, chain YC [auth A] mmCIF format, chain Z [auth A] mmCIF format, chain ZA [auth A] mmCIF format, chain ZB [auth A] mmCIF format, chain ZC [auth A]	AA [auth A] AB [auth A] AC [auth A] AD [auth A] BA [auth A] AA [auth A], AB [auth A], AC [auth A], AD [auth A], BA [auth A], BB [auth A], BC [auth A], BD [auth A], CA [auth A], CB [auth A], CC [auth A], CD [auth A], DA [auth A], DB [auth A], DC [auth A], DD [auth A], EA [auth A], EB [auth A], EC [auth A], ED [auth A], FA [auth A], FB [auth A], FC [auth A], FD [auth O], GA [auth A], GB [auth A], GC [auth A], HA [auth A], HB [auth A], HC [auth A], IA [auth A], IB [auth A], IC [auth A], JA [auth A], JB [auth A], JC [auth A], KA [auth A], KB [auth A], KC [auth A], LA [auth A], LB [auth A], LC [auth A], MA [auth A], MB [auth A], MC [auth A], NA [auth A], NB [auth A], NC [auth A], OA [auth A], OB [auth A], OC [auth A], PA [auth A], PB [auth A], PC [auth A], QA [auth A], QB [auth A], QC [auth A], RA [auth A], RB [auth A], RC [auth A], SA [auth A], SB [auth A], SC [auth A], TA [auth A], TB [auth A], TC [auth A], UA [auth A], UB [auth A], UC [auth A], VA [auth A], VB [auth A], VC [auth A], WA [auth A], WB [auth A], WC [auth A], XA [auth A], XB [auth A], XC [auth A], Y [auth A], YA [auth A], YB [auth A], YC [auth A], Z [auth A], ZA [auth A], ZB [auth A], ZC [auth A]	MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N		Interactions Focus chain AA [auth A] Focus chain AB [auth A] Focus chain AC [auth A] Focus chain AD [auth A] Focus chain BA [auth A] Focus chain BB [auth A] Focus chain BC [auth A] Focus chain BD [auth A] Focus chain CA [auth A] Focus chain CB [auth A] Focus chain CC [auth A] Focus chain CD [auth A] Focus chain DA [auth A] Focus chain DB [auth A] Focus chain DC [auth A] Focus chain DD [auth A] Focus chain EA [auth A] Focus chain EB [auth A] Focus chain EC [auth A] Focus chain ED [auth A] Focus chain FA [auth A] Focus chain FB [auth A] Focus chain FC [auth A] Focus chain FD [auth O] Focus chain GA [auth A] Focus chain GB [auth A] Focus chain GC [auth A] Focus chain HA [auth A] Focus chain HB [auth A] Focus chain HC [auth A] Focus chain IA [auth A] Focus chain IB [auth A] Focus chain IC [auth A] Focus chain JA [auth A] Focus chain JB [auth A] Focus chain JC [auth A] Focus chain KA [auth A] Focus chain KB [auth A] Focus chain KC [auth A] Focus chain LA [auth A] Focus chain LB [auth A] Focus chain LC [auth A] Focus chain MA [auth A] Focus chain MB [auth A] Focus chain MC [auth A] Focus chain NA [auth A] Focus chain NB [auth A] Focus chain NC [auth A] Focus chain OA [auth A] Focus chain OB [auth A] Focus chain OC [auth A] Focus chain PA [auth A] Focus chain PB [auth A] Focus chain PC [auth A] Focus chain QA [auth A] Focus chain QB [auth A] Focus chain QC [auth A] Focus chain RA [auth A] Focus chain RB [auth A] Focus chain RC [auth A] Focus chain SA [auth A] Focus chain SB [auth A] Focus chain SC [auth A] Focus chain TA [auth A] Focus chain TB [auth A] Focus chain TC [auth A] Focus chain UA [auth A] Focus chain UB [auth A] Focus chain UC [auth A] Focus chain VA [auth A] Focus chain VB [auth A] Focus chain VC [auth A] Focus chain WA [auth A] Focus chain WB [auth A] Focus chain WC [auth A] Focus chain XA [auth A] Focus chain XB [auth A] Focus chain XC [auth A] Focus chain Y [auth A] Focus chain YA [auth A] Focus chain YB [auth A] Focus chain YC [auth A] Focus chain Z [auth A] Focus chain ZA [auth A] Focus chain ZB [auth A] Focus chain ZC [auth A] Interactions & Density Focus chain AA [auth A] Focus chain AB [auth A] Focus chain AC [auth A] Focus chain AD [auth A] Focus chain BA [auth A] Focus chain BB [auth A] Focus chain BC [auth A] Focus chain BD [auth A] Focus chain CA [auth A] Focus chain CB [auth A] Focus chain CC [auth A] Focus chain CD [auth A] Focus chain DA [auth A] Focus chain DB [auth A] Focus chain DC [auth A] Focus chain DD [auth A] Focus chain EA [auth A] Focus chain EB [auth A] Focus chain EC [auth A] Focus chain ED [auth A] Focus chain FA [auth A] Focus chain FB [auth A] Focus chain FC [auth A] Focus chain FD [auth O] Focus chain GA [auth A] Focus chain GB [auth A] Focus chain GC [auth A] Focus chain HA [auth A] Focus chain HB [auth A] Focus chain HC [auth A] Focus chain IA [auth A] Focus chain IB [auth A] Focus chain IC [auth A] Focus chain JA [auth A] Focus chain JB [auth A] Focus chain JC [auth A] Focus chain KA [auth A] Focus chain KB [auth A] Focus chain KC [auth A] Focus chain LA [auth A] Focus chain LB [auth A] Focus chain LC [auth A] Focus chain MA [auth A] Focus chain MB [auth A] Focus chain MC [auth A] Focus chain NA [auth A] Focus chain NB [auth A] Focus chain NC [auth A] Focus chain OA [auth A] Focus chain OB [auth A] Focus chain OC [auth A] Focus chain PA [auth A] Focus chain PB [auth A] Focus chain PC [auth A] Focus chain QA [auth A] Focus chain QB [auth A] Focus chain QC [auth A] Focus chain RA [auth A] Focus chain RB [auth A] Focus chain RC [auth A] Focus chain SA [auth A] Focus chain SB [auth A] Focus chain SC [auth A] Focus chain TA [auth A] Focus chain TB [auth A] Focus chain TC [auth A] Focus chain UA [auth A] Focus chain UB [auth A] Focus chain UC [auth A] Focus chain VA [auth A] Focus chain VB [auth A] Focus chain VC [auth A] Focus chain WA [auth A] Focus chain WB [auth A] Focus chain WC [auth A] Focus chain XA [auth A] Focus chain XB [auth A] Focus chain XC [auth A] Focus chain Y [auth A] Focus chain YA [auth A] Focus chain YB [auth A] Focus chain YC [auth A] Focus chain Z [auth A] Focus chain ZA [auth A] Focus chain ZB [auth A] Focus chain ZC [auth A]

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
D2T Query on D2T	M	L-PEPTIDE LINKING	C₅ H₉ N O₄ S		ASP

Experimental Data & Validation

Experimental Data

Method: ELECTRON MICROSCOPY
Resolution: 3.00 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2024-11-20

Deposition Author(s):

Rahil, H.

Webster, M.W.

Weixlbaumer, A.

Funding Organization	Location	Grant Number
European Research Council (ERC)	European Union	679734
Agence Nationale de la Recherche (ANR)	France	ANR-10-LABX-0030-INRT
Agence Nationale de la Recherche (ANR)	France	ANR-10-IDEX-0002-02
European Molecular Biology Organization (EMBO)	European Union	--
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	United States	R35GM131922
Wellcome Trust	United Kingdom	203149

Revision History (Full details and data files)

Version 1.0: 2024-11-20
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.

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Help and Resources

9GUV

30S mRNA delivery complex (closed-head)

Molecular basis of mRNA delivery to the bacterial ribosome

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