Download MendeleyActivity and X-ray crystal structure of candidate base catalyst mutants of glycoside hydrolase family 6 cellobiohydrolase
Yamaguchi, S., Sunagawa, N., Tachioka, M., Igarashi, K.To be published.
8X0Q
X-Ray crystal structure of glycoside hydrolase family 6 cellobiohydrolase from Phanerochaete chrysosporium PcCel6A C240S/C393S/D394C cocrystallized with cellotriose
- PDB DOI: https://doi.org/10.2210/pdb8X0Q/pdb
- Classification: HYDROLASE
- Organism(s): Phanerodontia chrysosporium
- Expression System: Komagataella pastoris
- Mutation(s): Yes
- Deposited: 2023-11-05 Released: 2024-11-20
- Funding Organization(s): Japan Society for the Promotion of Science (JSPS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.00 ?
- R-Value Free: 0.177 (Depositor), 0.177 (DCC)
- R-Value Work: 0.157 (Depositor), 0.157 (DCC)
- R-Value Observed: 0.158 (Depositor)
Starting Model: experimental
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This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Glucanase | 358 | Phanerodontia chrysosporium | Mutation(s): 3 Gene Names: cel6A EC: 3.2.1 |  | |
UniProt | |||||
Find proteins for Q02321 (Phanerodontia chrysosporium) Explore Q02321 Go to UniProtKB: Q02321 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q02321 | ||||
Glycosylation | |||||
| Glycosylation Sites: 1 | |||||
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
| alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | B | 3 | N/A | N-Glycosylation | |
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
| beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | C | 2 | N/A | N/A | |
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GLC (Subject of Investigation/LOI) Query on GLC | E [auth A] | alpha-D-glucopyranose C6 H12 O6 WQZGKKKJIJFFOK-DVKNGEFBSA-N |  | ||
| BGC (Subject of Investigation/LOI) Query on BGC | D [auth A] | beta-D-glucopyranose C6 H12 O6 WQZGKKKJIJFFOK-VFUOTHLCSA-N |  | ||
| Modified Residues 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Type | Formula | 2D Diagram | Parent |
| OCS Query on OCS | A | L-PEPTIDE LINKING | C3 H7 N O5 S |  | CYS |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.00 ?
- R-Value Free: 0.177 (Depositor), 0.177 (DCC)
- R-Value Work: 0.157 (Depositor), 0.157 (DCC)
- R-Value Observed: 0.158 (Depositor)
Space Group: P 21 21 21
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 54.632 | ¦Á = 90 |
| b = 67.564 | ¦Â = 90 |
| c = 88.429 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| PHENIX | refinement |
| XDS | data reduction |
| XDS | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-11-20 Deposition Author(s): Yamaguchi, S., Sunagawa, N., Tachioka, M., Igarashi, K.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Japan Society for the Promotion of Science (JSPS) | Japan | 22J12651 |
Revision History (Full details and data files)
- Version 1.0: 2024-11-20
Type: Initial release
