1IM5

Crystal Structure of Pyrazinamidase of Pyrococcus horikoshii in Complex with Zinc

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1im5a_	Alpha and beta proteins (a/b)	Isochorismatase-like hydrolases	Isochorismatase-like hydrolases	Isochorismatase-like hydrolases	Pyrazinamidase/nicotinamidase	(Pyrococcus horikoshii ) [TaxId: 53953 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Isochorismatase-like hydrolases	8024273	4000591	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Isochorismatase-like hydrolases	8036652	3001778	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Isochorismatase	e1im5A1	A: a/b three-layered sandwiches	X: Isochorismatase-like hydrolases (From Topology)	H: Isochorismatase-like hydrolases (From Topology)	T: Isochorismatase-like hydrolases	F: Isochorismatase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.850	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Isochorismatase-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00857	Isochorismatase domain (Isochorismatase)	Isochorismatase domain	This is a domain found in hydrolase enzymes. This domain is responsible for the conversion of isochorismate, in the presence of water, to 2,3-dihydroxybenzoate and pyruvate. It shows a typical helix-sheet-helix sandwich structural architecture, with ...	This is a domain found in hydrolase enzymes. This domain is responsible for the conversion of isochorismate, in the presence of water, to 2,3-dihydroxybenzoate and pyruvate. It shows a typical helix-sheet-helix sandwich structural architecture, with a central beta--sheet made of six parallel strands, three alpha -helices on one side and two on the other [3,4].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	180aa long hypothetical Pyrazinamidase/Nicotinamidase	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides hydrolase activity catalytic activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cation binding ion binding binding metal ion binding small molecule binding	nucleoside phosphate metabolic process nucleotide biosynthetic process pyridine-containing compound biosynthetic process primary metabolic process nucleotide metabolic process nucleobase-containing compound biosynthetic process nucleobase-containing compound metabolic process biosynthetic process organophosphate metabolic process nucleobase-containing small molecule metabolic process organophosphate biosynthetic process phosphate-containing compound metabolic process nucleoside phosphate biosynthetic process pyridine-containing compound metabolic process nucleoside phosphate metabolic process nucleotide biosynthetic process pyridine-containing compound biosynthetic process primary metabolic process nucleotide metabolic process nucleobase-containing compound biosynthetic process nucleobase-containing compound metabolic process biosynthetic process organophosphate metabolic process nucleobase-containing small molecule metabolic process organophosphate biosynthetic process phosphate-containing compound metabolic process nucleoside phosphate biosynthetic process pyridine-containing compound metabolic process metabolic process cellular process small molecule metabolic process phosphorus metabolic process pyridine nucleotide metabolic process pyridine nucleotide biosynthetic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000868	Isochorismatase-like domain	Domain
A	IPR052347	Isochorismatase Family Nicotinamidases	Family
A	IPR036380	Isochorismatase-like superfamily	Homologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	nicotinamidase M-CSA #716	Pyrazinamide (PZA) is a prodrug used in the treatment of tuberculosis. PZA is converted to the active pyrazinoic acid by the bacterial PZAase enzyme; mutations in PZAase can therefore confer PZA resistance to M. tuberculosis. The gene product of Pyrococcus horikoshii 999 (PH999) shows PZAase and nicotinamidase activity, and extensive sequence homology to M. tuberculosis PZAase.	Defined by 9 residues: ASP:A-10ASP:A-52HIS:A-54SER:A-60HIS:A-71LYS:A-94GLU:A-101ALA:A-129CYS:A-133 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.5.1.19 (PDB Primary Data) Search M-CSA Motif + EC 3.5.1.19

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.