1BRD
Model for the structure of Bacteriorhodopsin based on high-resolution Electron Cryo-microscopy
ELECTRON CRYSTALLOGRAPHY
Crystal Data
| Unit Cell | |
|---|---|
| Length ( ? ) | Angle ( ? ) |
| a = 62.45 | ¦Á = 90 |
| b = 62.45 | ¦Â = 90 |
| c = 100 | ¦Ã = 120 |
| Symmetry | |
|---|---|
| Space Group | P 3 |
Diffraction
| Diffraction Experiment | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ID # | Crystal ID | Scattering Type | Data Collection Temperature | Detector | Detector Type | Details | Collection Date | Monochromator | Protocol | |||||
| 1 | 1 | electron | ||||||||||||
Refinement
| Statistics | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Diffraction ID | Structure Solution Method | Resolution (High) | Resolution (Low) | Number Reflections (R-Free) | Percent Reflections (Observed) | R-Work | R-Free | Mean Isotropic B | |||||||||||
| ELECTRON CRYSTALLOGRAPHY | 3.5 | ||||||||||||||||||
| Temperature Factor Modeling | ||||||
|---|---|---|---|---|---|---|
| Anisotropic B[1][1] | Anisotropic B[1][2] | Anisotropic B[1][3] | Anisotropic B[2][2] | Anisotropic B[2][3] | Anisotropic B[3][3] | |
| Non-Hydrogen Atoms Used in Refinement | |
|---|---|
| Non-Hydrogen Atoms | Number |
| Protein Atoms | 1343 |
| Nucleic Acid Atoms | |
| Solvent Atoms | |
| Heterogen Atoms | 20 |
| Sample |
|---|
| Bacteriorhodopsin from purple membrane |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | 2D ARRAY |
| Embedding Material | glucose |
| Embedding Details | 0.8% (w/v) glucose |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | CRYSTALLOGRAPHY |
| Number of Particles | |
| Reported Resolution (?) | 3.5 |
| Resolution Method | DIFFRACTION PATTERN/LAYERLINES |
| Other Details | |
| Refinement Type | |
| Symmetry Type | 2D CRYSTAL |
| Space Group Name | P 3 |
| Length a | 62.45 |
| Length b | 62.45 |
| Angle Gamma | 120 |
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | GENERIC FILM | GENERIC FILM | GENERIC FILM | ||||||
| Electron Dose (electrons/?**2) | 20 | 15 | |||||||
| Imaging Experiment | 1 | 2 | 3 |
|---|---|---|---|
| Date of Experiment | |||
| Temperature (Kelvin) | |||
| Microscope Model | FEI/PHILIPS EM420 | SIEMENS SULEIKA | JEOL 100B |
| Minimum Defocus (nm) | |||
| Maximum Defocus (nm) | |||
| Minimum Tilt Angle (degrees) | |||
| Maximum Tilt Angle (degrees) | |||
| Nominal CS | |||
| Imaging Mode | DIFFRACTION | BRIGHT FIELD | BRIGHT FIELD |
| Specimen Holder Model | |||
| Nominal Magnification | 66000 | 55000 | |
| Calibrated Magnification | |||
| Source | FIELD EMISSION GUN | ||
| Acceleration Voltage (kV) | 120 | 100 | 100 |
| Imaging Details | 60 degree tilted specimens | 0, 20, 45 degree + random degree tilts | , 20, 45 degree + random degree tilts |
