SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D DQF-COSY | 1 mM D arm tRNA(Met) | 100% D2O | 6.0 | ambient | 298 | ||
| 2 | 2D 1H-13C HSQC aliphatic | 1 mM D arm tRNA(Met) | 100% D2O | 6.0 | ambient | 298 | ||
| 3 | 2D 1H-13C HSQC aromatic | 1 mM D arm tRNA(Met) | 100% D2O | 6.0 | ambient | 298 | ||
| 4 | 2D 1H-1H NOESY | 1 mM D arm tRNA(Met) | 100% D2O | 6.0 | ambient | 298 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| molecular dynamics | TopSpin | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 10 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | data analysis | TopSpin | 2.1 | Bruker Biospin |
| 2 | structure solution | X-PLOR NIH | 3.851 | Schwieters, Kuszewski, Tjandra and Clore |
| 3 | refinement | X-PLOR NIH | Schwieters, Kuszewski, Tjandra and Clore | |
