2D9G

Solution structure of the zf-RanBP domain of YY1-associated factor 2

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_15N-separated_NOESY	1.05mM 13C, 15N-labeled protein; 20mM d-Tris-HCl (pH7.0); 100mM NaCl; 1mM d-DTT; 0.02%NaN3; 50uM ZnCl2+1mM IDA; 90%H2O, 10%D2O	90% H2O/10% D2O	120mM	7.0	ambient	298
2	3D_13C-separated_NOESY	1.05mM 13C, 15N-labeled protein; 20mM d-Tris-HCl (pH7.0); 100mM NaCl; 1mM d-DTT; 0.02%NaN3; 50uM ZnCl2+1mM IDA; 90%H2O, 10%D2O	90% H2O/10% D2O	120mM	7.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	JEOL	ECA	700

NMR Refinement
Method	Details	Software
torsion angle dynamics		Delta NMR

NMR Ensemble Information
Conformer Selection Criteria	target function,structures with the lowest energy,structures with the least restraint violations
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	Delta NMR	4.3	JEOL
2	processing	NMRPipe	20031121	Delaglio, F.
3	data analysis	NMRPipe	5.04	Johnson, B.A.
4	data analysis	KUJIRA	0.93191	Kobayashi, N.
5	structure solution	CYANA	2.0.17	Guntent, P.
6	refinement	CYANA	2.0.17	Guntent, P.

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.