Download MendeleyHigh Resolution Structure of E28A Mutant Benzoylformate Decarboxylase from Pseudomonas Putida
Bera, A.K., Anderson, N.L., Hasson, M.S.To be published.
1PO7
HIGH RESOLUTION STRUCTURE OF E28A MUTANT BENZOYLFORMATE DECARBOXYLASE FROM PSEUDOMONAS PUTIDA
- PDB DOI: https://doi.org/10.2210/pdb1PO7/pdb
- Classification: LYASE
- Organism(s): Pseudomonas putida
- Expression System: Pseudomonas putida
- Mutation(s): Yes
- Deposited: 2003-06-13 Released: 2004-11-23
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.20 ?
- R-Value Free: 0.142
- R-Value Work: 0.113
Starting Model: experimental
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This is version 1.5 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Benzoylformate decarboxylase | 528 | Pseudomonas putida | Mutation(s): 1 Gene Names: MDLC EC: 4.1.1.7 |  | |
UniProt | |||||
Find proteins for P20906 (Pseudomonas putida) Explore P20906 Go to UniProtKB: P20906 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P20906 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| TZD Query on TZD | F [auth A] | 2-{3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-4-METHYL-2-OXO-2,3-DIHYDRO-1,3-THIAZOL-5-YL}ETHYL TRIHYDROGEN
DIPHOSPHATE C12 H18 N4 O8 P2 S ZGJUYGIRPQSCFA-UHFFFAOYSA-N |  | ||
| CA Query on CA | C [auth A], D [auth A], E [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
| MG Query on MG | B [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.20 ?
- R-Value Free: 0.142
- R-Value Work: 0.113
- Space Group: I 2 2 2
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 81.127 | ¦Á = 90 |
| b = 95.543 | ¦Â = 90 |
| c = 137.388 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| SHELXL-97 | refinement |
| SCALEPACK | data scaling |
| CNS | refinement |
| SHELXL-97 | model building |
| DENZO | data reduction |
| SHELXL-97 | phasing |
| CNS | phasing |
Entry History
Deposition Data
- Released Date: 2004-11-23 Deposition Author(s): Bera, A.K., Hasson, M.S.
Revision History (Full details and data files)
- Version 1.0: 2004-11-23
Type: Initial release - Version 1.1: 2008-04-29
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Derived calculations, Version format compliance - Version 1.3: 2019-07-24
Changes: Advisory, Data collection, Refinement description - Version 1.4: 2021-10-27
Changes: Advisory, Database references, Derived calculations - Version 1.5: 2023-08-16
Changes: Data collection, Refinement description
