Download MendeleyStructural plasticity is key to multiple ligand binding by the multidrug binding regulator EbrR
Dong, J., Ni, L., Schumacher, M., Brennan, R.To be published.
3HTI
Crystal structure of multidrug binding protein EbrR complexed with malachite green
- PDB DOI: https://doi.org/10.2210/pdb3HTI/pdb
- Classification: DNA BINDING PROTEIN
- Organism(s): Streptomyces lividans
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2009-06-11 Released: 2010-07-14
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.50 ?
- R-Value Free: 0.286
- R-Value Work: 0.254
- R-Value Observed: 0.254
Starting Model: experimental
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This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| EbrA repressor | 217 | Streptomyces lividans | Mutation(s): 0 Gene Names: ebrR |  | |
UniProt | |||||
Find proteins for Q79SH7 (Streptomyces lividans) Explore Q79SH7 Go to UniProtKB: Q79SH7 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q79SH7 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MGR Query on MGR | B [auth A] | MALACHITE GREEN C23 H25 N2 VFCNQNZNPKRXIT-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.50 ?
- R-Value Free: 0.286
- R-Value Work: 0.254
- R-Value Observed: 0.254
- Space Group: P 31 2 1
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 59.91 | ¦Á = 90 |
| b = 59.91 | ¦Â = 90 |
| c = 110.236 | ¦Ă = 120 |
| Software Name | Purpose |
|---|---|
| CrystalClear | data collection |
| PHASER | phasing |
| CNS | refinement |
| d*TREK | data reduction |
| d*TREK | data scaling |
Entry History
Deposition Data
- Released Date: 2010-07-14 Deposition Author(s): Dong, J., Ni, L., Schumacher, M., Brennan, R.
Revision History (Full details and data files)
- Version 1.0: 2010-07-14
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2023-09-06
Changes: Data collection, Database references, Derived calculations, Refinement description
