Download MendeleyRetrofitting antibodies with stabilizing mutations
Langley, D.B., Roome, B., Christ, D.To be published.
4X4X
Retrofitting antibodies with stabilizing mutations. Herceptin scFv mutant.
- PDB DOI: https://doi.org/10.2210/pdb4X4X/pdb
- Classification: IMMUNE SYSTEM
- Organism(s): Homo sapiens
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2014-12-04 Released: 2015-12-23
- Funding Organization(s): National Health and Medical Research Council (NHMRC, Australia)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.25 ?
- R-Value Free: 0.269 (Depositor), 0.270 (DCC)
- R-Value Work: 0.226 (Depositor), 0.230 (DCC)
- R-Value Observed: 0.228 (Depositor)
wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Human Variable Heavy Chain of Herceptin | 140 | Homo sapiens | Mutation(s): 0 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Human Variable Light Chain of Herceptin | 107 | Homo sapiens | Mutation(s): 0 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.25 ?
- R-Value Free: 0.269 (Depositor), 0.270 (DCC)
- R-Value Work: 0.226 (Depositor), 0.230 (DCC)
- R-Value Observed: 0.228 (Depositor)
Space Group: P 31 2 1
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 91.19 | ¦Á = 90 |
| b = 91.19 | ¦Â = 90 |
| c = 114.35 | ¦Ă = 120 |
| Software Name | Purpose |
|---|---|
| Blu-Ice | data collection |
| MOSFLM | data reduction |
| SCALA | data scaling |
| PHASER | phasing |
| REFMAC | refinement |
Entry History & Funding Information
Deposition Data
- Released Date: 2015-12-23 Deposition Author(s): Langley, D.B., Roome, B., Christ, D.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Health and Medical Research Council (NHMRC, Australia) | Australia | -- |
Revision History (Full details and data files)
- Version 1.0: 2015-12-23
Type: Initial release - Version 1.1: 2017-09-06
Changes: Author supporting evidence, Data collection, Derived calculations - Version 1.2: 2020-01-08
Changes: Author supporting evidence - Version 1.3: 2024-10-30
Changes: Data collection, Database references, Structure summary
