Download MendeleyBenzoylformate decarboxylase F464I and A460V mutant from Pseudomonas putida
Bera, A.K., Hasson, M.S.To be published.
5DGD
Benzoylformate decarboxylase F464I and A460V mutant from Pseudomonas putida
- PDB DOI: https://doi.org/10.2210/pdb5DGD/pdb
- Classification: LYASE
- Organism(s): Pseudomonas putida
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2015-08-27 Released: 2015-09-16
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.13 ?
- R-Value Free: 0.150
- R-Value Work: 0.142
- R-Value Observed: 0.142
Starting Model: experimental
View more details
This is version 2.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Benzoylformate decarboxylase | 524 | Pseudomonas putida | Mutation(s): 2 Gene Names: mdlC EC: 4.1.1.7 |  | |
UniProt | |||||
Find proteins for P20906 (Pseudomonas putida) Explore P20906 Go to UniProtKB: P20906 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P20906 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| TPP Query on TPP | H [auth A] | THIAMINE DIPHOSPHATE C12 H19 N4 O7 P2 S AYEKOFBPNLCAJY-UHFFFAOYSA-O |  | ||
| CA Query on CA | E [auth A], F [auth A], G [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
| MG Query on MG | B [auth A], C [auth A], D [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.13 ?
- R-Value Free: 0.150
- R-Value Work: 0.142
- R-Value Observed: 0.142
- Space Group: I 2 2 2
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 81.5 | ¦Á = 90 |
| b = 95.656 | ¦Â = 90 |
| c = 137.345 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| SCALEPACK | data scaling |
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| HKL-2000 | data reduction |
| CNS | phasing |
Entry History
Deposition Data
- Released Date: 2015-09-16 Deposition Author(s): Bera, A.K., Hasson, M.S.
Revision History (Full details and data files)
- Version 1.0: 2015-09-16
Type: Initial release - Version 2.0: 2021-08-04
Changes: Atomic model, Data collection, Derived calculations, Non-polymer description, Structure summary - Version 2.1: 2023-09-27
Changes: Data collection, Database references, Refinement description
