Download MendeleySynergistic Lethality of a Binary Inhibitor of Mycobacterium tuberculosis KasA.
Kumar, P., Capodagli, G.C., Awasthi, D., Shrestha, R., Maharaja, K., Sukheja, P., Li, S.G., Inoyama, D., Zimmerman, M., Ho Liang, H.P., Sarathy, J., Mina, M., Rasic, G., Russo, R., Perryman, A.L., Richmann, T., Gupta, A., Singleton, E., Verma, S., Husain, S., Soteropoulos, P., Wang, Z., Morris, R., Porter, G., Agnihotri, G., Salgame, P., Ekins, S., Rhee, K.Y., Connell, N., Dartois, V., Neiditch, M.B., Freundlich, J.S., Alland, D.(2018) mBio 9
- PubMed: 30563908
- DOI: https://doi.org/10.1128/mBio.02101-17
- Primary Citation of Related Structures:
5W2O, 5W2P, 5W2Q, 5W2S - PubMed Abstract:
We report GSK3011724A (DG167) as a binary inhibitor of β-ketoacyl-ACP synthase (KasA) in Mycobacterium tuberculosis Genetic and biochemical studies established KasA as the primary target. The X-ray crystal structure of the KasA-DG167 complex refined to 2.0-? resolution revealed two interacting DG167 molecules occupying nonidentical sites in the substrate-binding channel of KasA. The binding affinities of KasA to DG167 and its analog, 5g, which binds only once in the substrate-binding channel, were determined, along with the KasA-5g X-ray crystal structure. DG167 strongly augmented the in vitro activity of isoniazid (INH), leading to synergistic lethality, and also synergized in an acute mouse model of M. tuberculosis infection. Synergistic lethality correlated with a unique transcriptional signature, including upregulation of oxidoreductases and downregulation of molecular chaperones. The lead structure-activity relationships (SAR), pharmacokinetic profile, and detailed interactions with the KasA protein that we describe may be applied to evolve a next-generation therapeutic strategy for tuberculosis (TB). IMPORTANCE Cell wall biosynthesis inhibitors have proven highly effective for treating tuberculosis (TB). We discovered and validated members of the indazole sulfonamide class of small molecules as inhibitors of Mycobacterium tuberculosis KasA-a key component for biosynthesis of the mycolic acid layer of the bacterium's cell wall and the same pathway as that inhibited by the first-line antitubercular drug isoniazid (INH). One lead compound, DG167, demonstrated synergistic lethality in combination with INH and a transcriptional pattern consistent with bactericidality and loss of persisters. Our results also detail a novel dual-binding mechanism for this compound as well as substantial structure-activity relationships (SAR) that may help in lead optimization activities. Together, these results suggest that KasA inhibition, specifically, that shown by the DG167 series, may be developed into a potent therapy that can synergize with existing antituberculars.
Organizational Affiliation:
Division of Infectious Disease, Department of Medicine, and the Ruy V. Lourenco Center for the Study of Emerging and Reemerging Pathogens, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, USA.
