Download MendeleyThe Role of Asp-Asp Short Hydrogen Bond in Maintaining Active Site Integrity of CTX-M Beta-Lactamase
Nichols, D.A., Kemp, M.T., Chen, Y.To be published.
6D7H
CTX-M-14 Apoenzyme
- PDB DOI: https://doi.org/10.2210/pdb6D7H/pdb
- Classification: HYDROLASE
- Organism(s): Escherichia coli
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2018-04-24 Released: 2019-07-24
- Funding Organization(s): National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 ?
- R-Value Free: 0.236
- R-Value Work: 0.183
- R-Value Observed: 0.188
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 2.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Beta-lactamase | 263 | Escherichia coli | Mutation(s): 0 Gene Names: blaCTX-M-14, beta-lactamase CTX-M-14, bla, bla CTX-M-14, bla-CTX-M-14a, blaCTX-M, blaCTX-M-14a, blaCTX-M-14b, blaCTX-M-14c, blaCTX-M-27b... EC: 3.5.2.6 |  | |
UniProt | |||||
Find proteins for H6UQI0 (Escherichia coli) Explore H6UQI0 Go to UniProtKB: H6UQI0 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | H6UQI0 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PO4 Query on PO4 | B [auth A] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| K Query on K | C [auth A], D [auth A], E [auth A], F [auth A] | POTASSIUM ION K NPYPAHLBTDXSSS-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.80 ?
- R-Value Free: 0.236
- R-Value Work: 0.183
- R-Value Observed: 0.188
- Space Group: P 32 2 1
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 41.71 | ¦Á = 90 |
| b = 41.71 | ¦Â = 90 |
| c = 232.524 | ¦Ă = 120 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| PDB_EXTRACT | data extraction |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2019-07-24 Deposition Author(s): Kemp, M., Nichols, D., Chen, Y.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) | United States | AI103158 |
Revision History (Full details and data files)
- Version 1.0: 2019-07-24
Type: Initial release - Version 1.1: 2019-12-18
Changes: Author supporting evidence - Version 2.0: 2022-07-06
Type: Coordinate replacement
Reason: Model completeness
Changes: Advisory, Atomic model, Data collection, Database references, Derived calculations, Polymer sequence, Refinement description, Source and taxonomy, Structure summary - Version 2.1: 2023-10-04
Changes: Data collection, Refinement description
