Download MendeleyCharacterization of the sorbitol dehydrogenase SmoS from Sinorhizobium meliloti 1021
Kohlmeier, M.G., Bailey-Elkin, B.A., Mark, B.L., Oresnik, I.J.(2021) Acta Crystallogr D Biol Crystallogr 77: 380-390
6PEJ
Structure of sorbitol dehydrogenase from Sinorhizobium meliloti 1021 bound to sorbitol
- PDB DOI: https://doi.org/10.2210/pdb6PEJ/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Sinorhizobium meliloti 1021
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2019-06-20 Released: 2020-06-24
- Funding Organization(s): Natural Sciences and Engineering Research Council (NSERC, Canada)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 ?
- R-Value Free: 0.249
- R-Value Work: 0.194
- R-Value Observed: 0.197
Starting Model: experimental
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This is version 1.3 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Sorbitol dehydrogenase (L-iditol 2-dehydrogenase) | 291 | Sinorhizobium meliloti 1021 | Mutation(s): 0 Gene Names: smoS, SMc01500 EC: 1.1.1.14 |  | |
UniProt | |||||
Find proteins for Q92N06 (Rhizobium meliloti (strain 1021)) Explore Q92N06 Go to UniProtKB: Q92N06 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q92N06 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SOR (Subject of Investigation/LOI) Query on SOR | E [auth A], F [auth B], G [auth C], H [auth D] | sorbitol C6 H14 O6 FBPFZTCFMRRESA-JGWLITMVSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 ?
- R-Value Free: 0.249
- R-Value Work: 0.194
- R-Value Observed: 0.197
- Space Group: P 1 21 1
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 83.298 | ¦Á = 90 |
| b = 88.298 | ¦Â = 117.39 |
| c = 87.317 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| XDS | data reduction |
| XDS | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2020-06-24 Deposition Author(s): Bailey-Elkin, B.A., Kohlmeier, M.G., Oresnik, I.J., Mark, B.L.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Natural Sciences and Engineering Research Council (NSERC, Canada) | Canada | -- |
Revision History (Full details and data files)
- Version 1.0: 2020-06-24
Type: Initial release - Version 1.1: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Derived calculations, Structure summary - Version 1.2: 2021-03-10
Changes: Database references, Structure summary - Version 1.3: 2023-10-11
Changes: Data collection, Database references, Refinement description
