Download MendeleyCrystal structure of aminoglycoside resistance enzyme ApmA, complex with apramycin
Stogios, P.J.To be published.
7JM2
Crystal structure of aminoglycoside resistance enzyme ApmA, complex with apramycin
- PDB DOI: https://doi.org/10.2210/pdb7JM2/pdb
- Classification: TRANSFERASE
- Organism(s): Staphylococcus aureus
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2020-07-30 Released: 2020-09-16
- Funding Organization(s): National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 ?
- R-Value Free: 0.236
- R-Value Work: 0.197
- R-Value Observed: 0.198
Starting Model: experimental
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This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Aminocyclitol acetyltransferase ApmA | 274 | Staphylococcus aureus | Mutation(s): 0 Gene Names: apmA |  | |
UniProt | |||||
Find proteins for A0A1D0AST6 (Staphylococcus aureus) Explore A0A1D0AST6 Go to UniProtKB: A0A1D0AST6 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A1D0AST6 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| AM2 (Subject of Investigation/LOI) Query on AM2 | D [auth A], E [auth B], G [auth C] | APRAMYCIN C21 H41 N5 O11 XZNUGFQTQHRASN-XQENGBIVSA-N |  | ||
| CL Query on CL | F [auth B], H [auth C] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 ?
- R-Value Free: 0.236
- R-Value Work: 0.197
- R-Value Observed: 0.198
- Space Group: P 21 21 21
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 61.73 | ¦Á = 90 |
| b = 107.536 | ¦Â = 90 |
| c = 138.696 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| HKL-3000 | data reduction |
| HKL-3000 | data scaling |
| PHENIX | phasing |
| PHENIX | model building |
| Coot | model building |
Entry History & Funding Information
Deposition Data
- Released Date: 2020-09-16 Deposition Author(s): Stogios, P.J., Evdokimova, E., Di Leo, R., Bordeleau, E., Wright, G.D., Savchenko, A., Joachimiak, A., Satchell, K.J.F., Center for Structural Genomics of Infectious Diseases (CSGID)
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) | United States | HHSN272201700060C |
Revision History (Full details and data files)
- Version 1.0: 2020-09-16
Type: Initial release - Version 1.1: 2023-10-18
Changes: Data collection, Database references, Refinement description
