Download MendeleyCrystal structure of Paradendryphiella salina PL7A alginate lyase in complex with tri-mannuronic acid
Fredslund, F., Welner, D.H., Wilkens, C.To be published.
7OOF
Crystal structure of Paradendryphiella salina PL7A alginate lyase in complex with tri-mannuronic acid
- PDB DOI: https://doi.org/10.2210/pdb7OOF/pdb
- Classification: LYASE
- Organism(s): Paradendryphiella salina
- Expression System: Komagataella pastoris
- Mutation(s): No
- Deposited: 2021-05-27 Released: 2022-06-08
- Funding Organization(s): European Union (EU)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.30 ?
- R-Value Free: 0.174
- R-Value Work: 0.159
- R-Value Observed: 0.159
Starting Model: experimental
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This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Alginate lyase (PL7) | 231 | Paradendryphiella salina | Mutation(s): 0 Gene Names: PsAlg7A |  | |
UniProt | |||||
Find proteins for A0A485PVH1 (Paradendryphiella salina) Explore A0A485PVH1 Go to UniProtKB: A0A485PVH1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A485PVH1 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| PG4 Query on PG4 | D [auth A] | TETRAETHYLENE GLYCOL C8 H18 O5 UWHCKJMYHZGTIT-UHFFFAOYSA-N |  | ||
| MAV (Subject of Investigation/LOI) Query on MAV | C [auth A] | alpha-D-mannopyranuronic acid C6 H10 O7 AEMOLEFTQBMNLQ-BYHBOUFCSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.30 ?
- R-Value Free: 0.174
- R-Value Work: 0.159
- R-Value Observed: 0.159
- Space Group: P 21 21 21
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 34.81 | ¦Á = 90 |
| b = 81.11 | ¦Â = 90 |
| c = 80.69 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| MxCuBE | data collection |
| XDS | data reduction |
| XDS | data scaling |
| Coot | model building |
| PHASER | phasing |
| PHENIX | refinement |
Entry History & Funding Information
Deposition Data
- Released Date: 2022-06-08 Deposition Author(s): Fredslund, F., Welner, D.H., Wilkens, C.
| Funding Organization | Location | Grant Number |
|---|---|---|
| European Union (EU) | Denmark | 9082-00021B |
Revision History (Full details and data files)
- Version 1.0: 2022-06-08
Type: Initial release - Version 1.1: 2024-01-31
Changes: Data collection, Refinement description - Version 1.2: 2024-11-20
Changes: Structure summary
