Download MendeleyInsulin single mutant INS-Q
Rao, S.S., Kundapura, S.V., Kulal, A., Ramagopal, U.A.To be published.
8HSK | pdb_00008hsk
Insulin single mutant INS-Q
- PDB DOI: https://doi.org/10.2210/pdb8HSK/pdb
- Classification: HORMONE
- Organism(s): Homo sapiens
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2022-12-19 Released: 2023-12-20
- Funding Organization(s): Not funded
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.64 ?
- R-Value Free: 0.165 (Depositor), 0.170 (DCC)
- R-Value Work: 0.153 (Depositor), 0.170 (DCC)
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by: Sequence | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Insulin A chain | 21 | Homo sapiens | Mutation(s): 0 Gene Names: INS |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P01308 (Homo sapiens) Explore P01308 Go to UniProtKB: P01308 | |||||
PHAROS: P01308 GTEx: ENSG00000254647 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P01308 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Insulin B chain | 30 | Homo sapiens | Mutation(s): 1 Gene Names: INS |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P01308 (Homo sapiens) Explore P01308 Go to UniProtKB: P01308 | |||||
PHAROS: P01308 GTEx: ENSG00000254647 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P01308 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | C [auth B], D [auth B] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.64 ?
- R-Value Free: 0.165 (Depositor), 0.170 (DCC)
- R-Value Work: 0.153 (Depositor), 0.170 (DCC)
Space Group: I 21 3
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 78.38 | ¦Á = 90 |
| b = 78.38 | ¦Â = 90 |
| c = 78.38 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| iMOSFLM | data reduction |
| SCALA | data scaling |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2023-12-20 Deposition Author(s): Rao, S.S., Kundapura, S.V., Kulal, A., Ramagopal, U.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Not funded | India | -- |
Revision History (Full details and data files)
- Version 1.0: 2023-12-20
Type: Initial release - Version 1.1: 2024-10-09
Changes: Structure summary
