9BNP | pdb_00009bnp

Cryo-EM structure of rhesus antibody V033-a.01 in complex with HIV-1 Env BG505 DS-SOSIP

PDB DOI: https://doi.org/10.2210/pdb9BNP/pdb
EM Map EMD-44733: EMDB EMDataResource

Classification: VIRAL PROTEIN/IMMUNE SYSTEM
Organism(s): Human immunodeficiency virus 1, Macaca mulatta
Expression System: Homo sapiens
Mutation(s): No

Deposited: 2024-05-02 Released: 2025-05-07
Deposition Author(s): Roark, R.S., Shapiro, L., Kwong, P.D.
Funding Organization(s): National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 3.17 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

HIV-1 neutralizing antibodies in SHIV-infected macaques recapitulate structurally divergent modes of human V2 apex recognition with a single D gene

Roark, R.S., Shapiro, L., Kwong, P.D.

To be published.

Macromolecule Content

Total Structure Weight: 254.57 kDa
Atom Count: 16,537
Modelled Residue Count: 1,920
Deposited Residue Count: 2,078
Unique protein chains: 4

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Envelope glycoprotein Gp120	A, C [auth E], F [auth I]	468	Human immunodeficiency virus 1	Mutation(s): 0 Gene Names: env
UniProt
Find proteins for Q2N0S6 (Human immunodeficiency virus type 1) Explore Q2N0S6 Go to UniProtKB: Q2N0S6
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q2N0S6
Glycosylation
Glycosylation Sites: 17	Glycosylation Focus chain A Focus chain C [auth E] Focus chain F [auth I]
Sequence Annotations Expand
Reference Sequence

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Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
Envelope glycoprotein Gp41	B, D [auth F], G [auth J]	145	Human immunodeficiency virus 1	Mutation(s): 0 Gene Names: env
UniProt
Find proteins for Q2N0S6 (Human immunodeficiency virus type 1) Explore Q2N0S6 Go to UniProtKB: Q2N0S6
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q2N0S6
Glycosylation
Glycosylation Sites: 2	Glycosylation Focus chain D [auth F] Focus chain G [auth J]
Sequence Annotations Expand
Reference Sequence

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(by identity cutoff) | 3D Structure

Entity ID: 3
Molecule	Chains	Sequence Length	Organism	Details	Image
V033-a.01 heavy chain	E [auth H]	132	Macaca mulatta	Mutation(s): 0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

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(by identity cutoff) | 3D Structure

Entity ID: 4
Molecule	Chains	Sequence Length	Organism	Details	Image
V033-a.01 light chain	H [auth L]	107	Macaca mulatta	Mutation(s): 0
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence

Oligosaccharides

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Entity ID: 5
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	BA [auth b], CA [auth c], DA [auth d], EA [auth e], FA [auth f], BA [auth b], CA [auth c], DA [auth d], EA [auth e], FA [auth f], GA [auth g], HA [auth h], I [auth C], IA [auth i], J [auth D], K [auth G], KA [auth k], M, N, NA [auth n], O, OA [auth o], P, PA [auth p], Q, QA [auth q], R, RA [auth r], S, T, U, V, X, Y	2		N-Glycosylation	Interactions Focus chain BA [auth b] Focus chain CA [auth c] Focus chain DA [auth d] Focus chain EA [auth e] Focus chain FA [auth f] Focus chain GA [auth g] Focus chain HA [auth h] Focus chain I [auth C] Focus chain IA [auth i] Focus chain J [auth D] Focus chain K [auth G] Focus chain KA [auth k] Focus chain M Focus chain N Focus chain NA [auth n] Focus chain O Focus chain OA [auth o] Focus chain P Focus chain PA [auth p] Focus chain Q Focus chain QA [auth q] Focus chain R Focus chain RA [auth r] Focus chain S Focus chain T Focus chain U Focus chain V Focus chain X Focus chain Y Interactions & Density Focus chain BA [auth b] Focus chain CA [auth c] Focus chain DA [auth d] Focus chain EA [auth e] Focus chain FA [auth f] Focus chain GA [auth g] Focus chain HA [auth h] Focus chain I [auth C] Focus chain IA [auth i] Focus chain J [auth D] Focus chain K [auth G] Focus chain KA [auth k] Focus chain M Focus chain N Focus chain NA [auth n] Focus chain O Focus chain OA [auth o] Focus chain P Focus chain PA [auth p] Focus chain Q Focus chain QA [auth q] Focus chain R Focus chain RA [auth r] Focus chain S Focus chain T Focus chain U Focus chain V Focus chain X Focus chain Y

Entity ID: 6
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	L [auth K], W	4		N-Glycosylation	Interactions Focus chain L [auth K] Focus chain W Interactions & Density Focus chain L [auth K] Focus chain W

Entity ID: 7
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	Z	6		N-Glycosylation	Interactions Focus chain Z Interactions & Density Focus chain Z

Entity ID: 8
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	AA [auth a]	7		N-Glycosylation	Interactions Focus chain AA [auth a] Interactions & Density Focus chain AA [auth a]

Entity ID: 9
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	JA [auth j], MA [auth m]	3		N-Glycosylation	Interactions Focus chain JA [auth j] Focus chain MA [auth m] Interactions & Density Focus chain JA [auth j] Focus chain MA [auth m]

Entity ID: 10
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	LA [auth l]	4		N-Glycosylation	Interactions Focus chain LA [auth l] Interactions & Density Focus chain LA [auth l]

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
NAG Query on NAG Download Ideal Coordinates CCD File SDF format, chain AB [auth E] SDF format, chain CB [auth F] SDF format, chain DB [auth I] SDF format, chain EB [auth I] SDF format, chain FB [auth I] SDF format, chain GB [auth I] SDF format, chain HB [auth I] SDF format, chain IB [auth J] SDF format, chain JB [auth J] SDF format, chain TA [auth A] SDF format, chain UA [auth A] SDF format, chain WA [auth A] SDF format, chain XA [auth A] SDF format, chain ZA [auth E] SDF format, chain BB [auth E] SDF format, chain SA [auth A] SDF format, chain VA [auth A] SDF format, chain YA [auth E] MOL2 format, chain AB [auth E] MOL2 format, chain CB [auth F] MOL2 format, chain DB [auth I] MOL2 format, chain EB [auth I] MOL2 format, chain FB [auth I] MOL2 format, chain GB [auth I] MOL2 format, chain HB [auth I] MOL2 format, chain IB [auth J] MOL2 format, chain JB [auth J] MOL2 format, chain TA [auth A] MOL2 format, chain UA [auth A] MOL2 format, chain WA [auth A] MOL2 format, chain XA [auth A] MOL2 format, chain ZA [auth E] MOL2 format, chain BB [auth E] MOL2 format, chain SA [auth A] MOL2 format, chain VA [auth A] MOL2 format, chain YA [auth E] mmCIF format, chain AB [auth E] mmCIF format, chain CB [auth F] mmCIF format, chain DB [auth I] mmCIF format, chain EB [auth I] mmCIF format, chain FB [auth I] mmCIF format, chain GB [auth I] mmCIF format, chain HB [auth I] mmCIF format, chain IB [auth J] mmCIF format, chain JB [auth J] mmCIF format, chain TA [auth A] mmCIF format, chain UA [auth A] mmCIF format, chain WA [auth A] mmCIF format, chain XA [auth A] mmCIF format, chain ZA [auth E] mmCIF format, chain BB [auth E] mmCIF format, chain SA [auth A] mmCIF format, chain VA [auth A] mmCIF format, chain YA [auth E]	AB [auth E] BB [auth E] CB [auth F] DB [auth I] EB [auth I] AB [auth E], BB [auth E], CB [auth F], DB [auth I], EB [auth I], FB [auth I], GB [auth I], HB [auth I], IB [auth J], JB [auth J], SA [auth A], TA [auth A], UA [auth A], VA [auth A], WA [auth A], XA [auth A], YA [auth E], ZA [auth E]	2-acetamido-2-deoxy-beta-D-glucopyranose C₈ H₁₅ N O₆ OVRNDRQMDRJTHS-FMDGEEDCSA-N		Interactions Focus chain AB [auth E] Focus chain BB [auth E] Focus chain CB [auth F] Focus chain DB [auth I] Focus chain EB [auth I] Focus chain FB [auth I] Focus chain GB [auth I] Focus chain HB [auth I] Focus chain IB [auth J] Focus chain JB [auth J] Focus chain SA [auth A] Focus chain TA [auth A] Focus chain UA [auth A] Focus chain VA [auth A] Focus chain WA [auth A] Focus chain XA [auth A] Focus chain YA [auth E] Focus chain ZA [auth E] Interactions & Density Focus chain AB [auth E] Focus chain BB [auth E] Focus chain CB [auth F] Focus chain DB [auth I] Focus chain EB [auth I] Focus chain FB [auth I] Focus chain GB [auth I] Focus chain HB [auth I] Focus chain IB [auth J] Focus chain JB [auth J] Focus chain SA [auth A] Focus chain TA [auth A] Focus chain UA [auth A] Focus chain VA [auth A] Focus chain WA [auth A] Focus chain XA [auth A] Focus chain YA [auth E] Focus chain ZA [auth E]