9BTU
Human SCNN1B-SCNN1G ENaC dimers
ELECTRON MICROSCOPY
Starting Model(s)
| Initial Refinement Model(s) | |||
|---|---|---|---|
| Type | Source | Accession Code | Details |
| experimental model | PDB | 6WTH | |
Refinement
| RMS Deviations | |
|---|---|
| Key | Refinement Restraint Deviation |
| f_dihedral_angle_d | 11.911 |
| f_angle_d | 0.78 |
| f_chiral_restr | 0.046 |
| f_bond_d | 0.005 |
| f_plane_restr | 0.005 |
| Sample |
|---|
| Amiloride-sensitive sodium channel subunits BETA, and GAMMA in complex with 10D4 Fab |
| Sample Components |
| Human beta subunit isoform 1 |
| Human gamma subunit isoform 1 |
| Fab fragment from 10D4 IgG |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK IV |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 248323 |
| Reported Resolution (?) | 3.68 |
| Resolution Method | FSC 0.5 CUT-OFF |
| Other Details | Local Resolution with one of the dimers and the Fabs masked out |
| Refinement Type | |
| Symmetry Type | POINT |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (6wth) | ||||
| Refinement Space | |||||
| Refinement Protocol | OTHER | ||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | |||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | FEI FALCON III (4k x 4k) | ||||||||
| Electron Dose (electrons/?**2) | 50 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | |
| Temperature (Kelvin) | |
| Microscope Model | FEI TITAN KRIOS |
| Minimum Defocus (nm) | 800 |
| Maximum Defocus (nm) | 2500 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2.7 |
| Imaging Mode | DIFFRACTION |
| Specimen Holder Model | |
| Nominal Magnification | |
| Calibrated Magnification | |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| PARTICLE SELECTION | cryoSPARC | 4.3.1 |
| IMAGE ACQUISITION | SerialEM | unk |
| CTF CORRECTION | cryoSPARC | 4.3.1 |
| MODEL FITTING | PHENIX | 1.2 |
| MODEL REFINEMENT | ISOLDE | 1.6 |
| MODEL REFINEMENT | PHENIX | 1.2 |
| INITIAL EULER ASSIGNMENT | cryoSPARC | 4.3.1 |
| FINAL EULER ASSIGNMENT | cryoSPARC | 4.3.1 |
| CLASSIFICATION | cryoSPARC | 4.3.1 |
| RECONSTRUCTION | cryoSPARC | 4.3.1 |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| PHASE FLIPPING AND AMPLITUDE CORRECTION | From blob picking in cryoSPARC with a box size of 440 with a maximum particle diameter of 250 and a minimum of 150. Minimum separation distance was 0.6 diameters | |||
