Download MendeleyStructural Insights into Subunit-Dependent Functional Regulation in Epithelial Sodium Channels
Houser, A., Baconguis, I.To be published.
9BTU
Human SCNN1B-SCNN1G ENaC dimers
- PDB DOI: https://doi.org/10.2210/pdb9BTU/pdb
- EM Map EMD-44896: EMDB EMDataResource
- Classification: MEMBRANE PROTEIN
- Organism(s): Homo sapiens, Mus musculus
- Expression System: Homo sapiens, Mus musculus
- Mutation(s): No
- Deposited: 2024-05-15 Released: 2024-11-27
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Cystic Fibrosis Foundation, National Science Foundation (NSF, United States)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 3.68 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Amiloride-sensitive sodium channel subunit beta | A [auth B] | 640 | Homo sapiens | Mutation(s): 0 Gene Names: SCNN1B |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P51168 (Homo sapiens) Explore P51168 Go to UniProtKB: P51168 | |||||
PHAROS: P51168 GTEx: ENSG00000168447 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P51168 | ||||
Glycosylation | |||||
| Glycosylation Sites: 4 | Go to GlyGen: P51168-1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| 10D4 Fab | B [auth D] | 116 | Mus musculus | Mutation(s): 0 |  |
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Amiloride-sensitive sodium channel subunit gamma | 649 | Homo sapiens | Mutation(s): 0 Gene Names: SCNN1G |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P51170 (Homo sapiens) Explore P51170 Go to UniProtKB: P51170 | |||||
PHAROS: P51170 GTEx: ENSG00000166828 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P51170 | ||||
Glycosylation | |||||
| Glycosylation Sites: 1 | Go to GlyGen: P51170-1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 4 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| 10D4 Fab | D [auth E] | 73 | Mus musculus | Mutation(s): 0 |  |
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Entity ID: 5 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
| beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | E [auth A], F [auth H], G [auth I] | 3 |  | N-Glycosylation | |
Glycosylation Resources | |||||
GlyTouCan: G15407YE GlyCosmos: G15407YE GlyGen: G15407YE | |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NAG Query on NAG | I [auth B], J [auth B], K [auth B], L [auth C], M [auth C] | 2-acetamido-2-deoxy-beta-D-glucopyranose C8 H15 N O6 OVRNDRQMDRJTHS-FMDGEEDCSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 3.68 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
| Task | Software Package | Version |
|---|---|---|
| MODEL REFINEMENT | ISOLDE | 1.6 |
| MODEL REFINEMENT | PHENIX | 1.2 |
| RECONSTRUCTION | cryoSPARC | 4.3.1 |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-11-27 Deposition Author(s): Houser, A., Baconguis, I.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | 1R01GM138862 |
| Cystic Fibrosis Foundation | United States | BACONG22G0 |
| National Science Foundation (NSF, United States) | United States | GVPRS0015B4 |
Revision History (Full details and data files)
- Version 1.0: 2024-11-27
Type: Initial release
