ELECTRON MICROSCOPY
Starting Model(s)
| Initial Refinement Model(s) | |||
|---|---|---|---|
| Type | Source | Accession Code | Details |
| experimental model | PDB | 3ZX8 | |
| Sample |
|---|
| Satellite tobacco necrosis virus 1 |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK IV |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 207142 |
| Reported Resolution (?) | 2.77 |
| Resolution Method | FSC 0.143 CUT-OFF |
| Other Details | |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | I |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (2BUK) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | FLEXIBLE FIT | ||||
| Refinement Target | cross-correlation coefficient | ||||
| Overall B Value | 43 | ||||
| Fitting Procedure | |||||
| Details | |||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | TFS FALCON 4i (4k x 4k) | TFS FALCON 4i (4k x 4k) | TFS FALCON 4i (4k x 4k) | ||||||
| Electron Dose (electrons/?**2) | 30 | 30 | 40 | ||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | |
| Temperature (Kelvin) | |
| Microscope Model | TFS TALOS F200C |
| Minimum Defocus (nm) | 700 |
| Maximum Defocus (nm) | 1800 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2.7 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | GATAN ELSA 698 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER |
| Nominal Magnification | 150000 |
| Calibrated Magnification | |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 200 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| PARTICLE SELECTION | cryoSPARC | 4.5.3 |
| IMAGE ACQUISITION | EPU | 3.7.1.6940 |
| CTF CORRECTION | cryoSPARC | 4.5.3 |
| MODEL FITTING | UCSF ChimeraX | 1.8 |
| MODEL FITTING | Coot | 0.9.8.95 EL (ccp4) |
| INITIAL EULER ASSIGNMENT | cryoSPARC | 4.5.3 |
| FINAL EULER ASSIGNMENT | cryoSPARC | 4.5.3 |
| CLASSIFICATION | cryoSPARC | 4.5.3 |
| RECONSTRUCTION | cryoSPARC | 4.5.3 |
| MODEL REFINEMENT | PHENIX | 1.21.2_5419: |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| PHASE FLIPPING AND AMPLITUDE CORRECTION | 2394377 | combined figure from the 3 datasets | ||
