Download MendeleyThe Crystal Structure of Influenza Type a Virus Neuraminidase of the N6 Subtype at 1.85 A Resolution
Rudino-Pinera, E., Crennell, S.J., Webster, R.G., Laver, W.G., Garman, E.F.To be published.
2C4L
Structure of Neuraminidase Subtype N9 Complexed with 30 MM Sialic Acid (NANA, NEU5AC), Crystal Soaked for 24 Hours at 291 K and Finally Backsoaked for 30 Min in a Cryoprotectant Solution which did not contain NEU5AC
- PDB DOI: https://doi.org/10.2210/pdb2C4L/pdb
- Classification: HYDROLASE
- Organism(s): Influenza A virus
- Expression System: Gallus gallus
- Mutation(s): No
- Deposited: 2005-10-20 Released: 2007-03-27
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.15 ?
- R-Value Free: 0.196
- R-Value Work: 0.155
- R-Value Observed: 0.157
Starting Model: experimental
View more details
This is version 2.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| NEURAMINIDASE SUBTYPE N9 | 388 | Influenza A virus | Mutation(s): 0 EC: 3.2.1.18 |  | |
UniProt | |||||
Find proteins for P03472 (Influenza A virus (strain A/Tern/Australia/G70C/1975 H11N9)) Explore P03472 Go to UniProtKB: P03472 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P03472 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Small Molecules
| Ligands 4 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SIA Query on SIA | C [auth A], D [auth A] | N-acetyl-alpha-neuraminic acid C11 H19 N O9 SQVRNKJHWKZAKO-YRMXFSIDSA-N |  | ||
| NAG Query on NAG | E [auth A], F [auth A], L [auth A], M [auth A], N [auth A] | 2-acetamido-2-deoxy-beta-D-glucopyranose C8 H15 N O6 OVRNDRQMDRJTHS-FMDGEEDCSA-N |  | ||
| BMA Query on BMA | G [auth A], H [auth A], I [auth A], J [auth A], K [auth A] | beta-D-mannopyranose C6 H12 O6 WQZGKKKJIJFFOK-RWOPYEJCSA-N |  | ||
| CA Query on CA | O [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Biologically Interesting Molecules (External Reference) 1 Unique
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| ID | Chains | Name | Type/Class | 2D Diagram | 3D Interactions |
| PRD_900111 Query on PRD_900111 | B | 2alpha-alpha-mannobiose | Oligosaccharide / Metabolism |  | |
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.15 ?
- R-Value Free: 0.196
- R-Value Work: 0.155
- R-Value Observed: 0.157
- Space Group: I 4 3 2
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 181.178 | ¦Á = 90 |
| b = 181.178 | ¦Â = 90 |
| c = 181.178 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
| MOLREP | phasing |
Entry History
Deposition Data
- Released Date: 2007-03-27 Deposition Author(s): Rudino-Pinera, E., Tunnah, P., Crennell, S.J., Webster, R.G., Laver, W.G., Garman, E.F.
Revision History (Full details and data files)
- Version 1.0: 2007-03-27
Type: Initial release - Version 1.1: 2011-05-08
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2018-06-20
Changes: Data collection, Structure summary - Version 1.4: 2020-07-01
Changes: Advisory, Data collection, Derived calculations, Other - Version 2.0: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Advisory, Atomic model, Data collection, Derived calculations, Structure summary - Version 2.1: 2023-12-13
Changes: Data collection, Database references, Refinement description, Structure summary - Version 2.2: 2024-10-09
Changes: Structure summary
