Download MendeleyImprovement of the Crystal Quality by the Surface Mutations on a Beta-Lactamase Toho-1
Shimamura, T., Nitanai, Y., Uchiyama, T., Ago, H., Matsuzawa, H., Miyano, M.To be published.
2ZQ7
Apo structure of Class A beta-lactamase Toho-1 E166A/R274N/R276N triple mutant
- PDB DOI: https://doi.org/10.2210/pdb2ZQ7/pdb
- Classification: HYDROLASE
- Organism(s): Escherichia coli
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2008-08-07 Released: 2009-07-28
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 0.94 ?
- R-Value Free: 0.147
- R-Value Work: 0.135
- R-Value Observed: 0.136
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Beta-lactamase Toho-1 | 262 | Escherichia coli | Mutation(s): 3 Gene Names: bla EC: 3.5.2.6 |  | |
UniProt | |||||
Find proteins for Q47066 (Escherichia coli) Explore Q47066 Go to UniProtKB: Q47066 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q47066 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | B [auth A], C [auth A], D [auth A], E [auth A], F [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 0.94 ?
- R-Value Free: 0.147
- R-Value Work: 0.135
- R-Value Observed: 0.136
- Space Group: P 32 2 1
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 72.545 | ¦Á = 90 |
| b = 72.545 | ¦Â = 90 |
| c = 97.656 | ¦Ă = 120 |
| Software Name | Purpose |
|---|---|
| MOLREP | phasing |
| REFMAC | refinement |
| HKL-2000 | data collection |
| HKL-2000 | data reduction |
| SCALEPACK | data scaling |
Entry History
Deposition Data
- Released Date: 2009-07-28 Deposition Author(s): Shimamura, T., Nitanai, Y., Uchiyama, T., Ago, H., Matsuzawa, H., Miyano, M.
Revision History (Full details and data files)
- Version 1.0: 2009-07-28
Type: Initial release - Version 1.1: 2011-07-13
Changes: Version format compliance - Version 1.2: 2021-11-10
Changes: Database references, Derived calculations - Version 1.3: 2023-11-01
Changes: Data collection, Refinement description
