Download MendeleyA fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin
Wang, Y., Luo, Z., Shi, X., Wang, H., Nie, L., Huang, M.(2011) Protein Sci 20: 2095-2101
- PubMed: 21997768
- DOI: https://doi.org/10.1002/pro.749
- Primary Citation of Related Structures:
3TDL - PubMed Abstract:
11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.
Organizational Affiliation:
State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China.
