Download MendeleyCrystal structure of S63A mutant of human macrophage migration inhibitory factor
Pantouris, G., Lolis, E.To be published.
5BSJ
Crystal structure of S63A mutant of human macrophage migration inhibitory factor
- PDB DOI: https://doi.org/10.2210/pdb5BSJ/pdb
- Classification: ISOMERASE
- Organism(s): Homo sapiens
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2015-06-02 Released: 2016-06-08
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 ?
- R-Value Free: 0.210
- R-Value Work: 0.183
- R-Value Observed: 0.184
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Macrophage migration inhibitory factor | 114 | Homo sapiens | Mutation(s): 1 Gene Names: MIF, GLIF, MMIF EC: 5.3.2.1 (PDB Primary Data), 5.3.3.12 (PDB Primary Data) |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P14174 (Homo sapiens) Explore P14174 Go to UniProtKB: P14174 | |||||
PHAROS: P14174 GTEx: ENSG00000240972 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P14174 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | D [auth A], E [auth A], F [auth A], L [auth C] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| GOL Query on GOL | I [auth B], J [auth C], K [auth C] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
| IPA Query on IPA | G [auth A], H [auth A] | ISOPROPYL ALCOHOL C3 H8 O KFZMGEQAYNKOFK-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.00 ?
- R-Value Free: 0.210
- R-Value Work: 0.183
- R-Value Observed: 0.184
- Space Group: P 31 2 1
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 95.785 | ¦Á = 90 |
| b = 95.785 | ¦Â = 90 |
| c = 103.889 | ¦Ă = 120 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data scaling |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
| HKL-2000 | data reduction |
| PHASER | phasing |
Entry History
Deposition Data
- Released Date: 2016-06-08 Deposition Author(s): Pantouris, G., Lolis, E.
Revision History (Full details and data files)
- Version 1.0: 2016-06-08
Type: Initial release - Version 1.1: 2023-09-27
Changes: Data collection, Database references, Derived calculations, Refinement description
