Download MendeleyStructure of GH32 from Bifidobacterium adolescentis in complex with fructose
Lima, M.Z.T., Muniz, J.R.C.To be published.
6NUN
Structure of GH32 hydrolase from Bifidobacterium adolescentis in complex with frutose
- PDB DOI: https://doi.org/10.2210/pdb6NUN/pdb
- Classification: HYDROLASE
- Organism(s): Bifidobacterium adolescentis
- Expression System: Escherichia coli
- Mutation(s): No
- Deposited: 2019-02-01 Released: 2020-02-05
- Funding Organization(s): Sao Paulo Research Foundation (FAPESP), Brazilian National Council for Scientific and Technological Development (CNPq)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.87 ?
- R-Value Free: 0.198
- R-Value Work: 0.161
- R-Value Observed: 0.163
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Beta-fructofuranosidase | 518 | Bifidobacterium adolescentis | Mutation(s): 0 Gene Names: B0703_1339 EC: 3.2.1.26 |  | |
UniProt | |||||
Find proteins for A1A2J8 (Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)) Explore A1A2J8 Go to UniProtKB: A1A2J8 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A1A2J8 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| FRU Query on FRU | G [auth A] | beta-D-fructofuranose C6 H12 O6 RFSUNEUAIZKAJO-ARQDHWQXSA-N |  | ||
| EDO Query on EDO | B [auth A], C [auth A], D [auth A], E [auth A], F [auth A] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.87 ?
- R-Value Free: 0.198
- R-Value Work: 0.161
- R-Value Observed: 0.163
- Space Group: P 31 2 1
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 86.98 | ¦Á = 90 |
| b = 86.98 | ¦Â = 90 |
| c = 115.5 | ¦Ă = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| TRUNCATE | data reduction |
| SAINT | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2020-02-05 Deposition Author(s): Lima, M.Z.T., Muniz, J.R.C.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Sao Paulo Research Foundation (FAPESP) | Brazil | 2017/16291-5 |
| Brazilian National Council for Scientific and Technological Development (CNPq) | Brazil | 309767/2015-6 |
| Brazilian National Council for Scientific and Technological Development (CNPq) | Brazil | 486546/2013-6 |
| Brazilian National Council for Scientific and Technological Development (CNPq) | Brazil | 308865/2018-9 |
Revision History (Full details and data files)
- Version 1.0: 2020-02-05
Type: Initial release - Version 1.1: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary - Version 1.2: 2024-03-13
Changes: Data collection, Database references, Structure summary
