Download MendeleyCrystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with hexa-mannuronic acid
Fredslund, F., Welner, D.H., Wilkens, C.To be published.
7NCZ
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with hexa-mannuronic acid
- PDB DOI: https://doi.org/10.2210/pdb7NCZ/pdb
- Classification: LYASE
- Organism(s): Paradendryphiella salina
- Expression System: Komagataella pastoris
- Mutation(s): Yes
- Deposited: 2021-01-29 Released: 2022-03-02
- Funding Organization(s): European Union (EU)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.64 ?
- R-Value Free: 0.190
- R-Value Work: 0.152
- R-Value Observed: 0.154
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Alginate lyase (PL7) | 231 | Paradendryphiella salina | Mutation(s): 1 Gene Names: PsAlg7A EC: 4.2.2.3 |  | |
UniProt | |||||
Find proteins for A0A485PVH1 (Paradendryphiella salina) Explore A0A485PVH1 Go to UniProtKB: A0A485PVH1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A485PVH1 | ||||
Sequence AnnotationsExpand | |||||
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Oligosaccharides
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | 2D Diagram | Glycosylation | 3D Interactions |
| beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid | B | 7 |  | N/A | |
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| BTB Query on BTB | C [auth A] | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL C8 H19 N O5 OWMVSZAMULFTJU-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.64 ?
- R-Value Free: 0.190
- R-Value Work: 0.152
- R-Value Observed: 0.154
- Space Group: P 21 21 21
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 34.81 | ¦Á = 90 |
| b = 80.9 | ¦Â = 90 |
| c = 81.21 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XDS | data reduction |
| XDS | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2022-03-02 Deposition Author(s): Fredslund, F., Welner, D.H., Wilkens, C.
| Funding Organization | Location | Grant Number |
|---|---|---|
| European Union (EU) | Denmark | 9082-00021B |
Revision History (Full details and data files)
- Version 1.0: 2022-03-02
Type: Initial release - Version 1.1: 2024-01-31
Changes: Data collection, Refinement description - Version 1.2: 2024-11-06
Changes: Structure summary
