Download Mendeley12 mutant Ribonuclease from Hericium erinaceus GMP binding form
Takebe, K., Chida, T., Suzuki, M., Uzawa, N., Kobayashi, H.To be published.
7W05
12 mutant Ribonuclease from Hericium erinaceus GMP binding form
- PDB DOI: https://doi.org/10.2210/pdb7W05/pdb
- Classification: DNA BINDING PROTEIN
- Organism(s): Hericium erinaceus
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2021-11-17 Released: 2022-11-23
- Funding Organization(s): Japan Society for the Promotion of Science (JSPS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.29 ?
- R-Value Free: 0.166
- R-Value Work: 0.137
- R-Value Observed: 0.138
Starting Model: experimental
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This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Ribonuclease T1 | 100 | Hericium erinaceus | Mutation(s): 12 Gene Names: RNHe1 |  | |
UniProt | |||||
Find proteins for B1Q4V2 (Hericium erinaceus) Explore B1Q4V2 Go to UniProtKB: B1Q4V2 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | B1Q4V2 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| GMP Query on GMP | B [auth A] | GUANOSINE C10 H13 N5 O5 NYHBQMYGNKIUIF-UUOKFMHZSA-N |  | ||
| PEG (Subject of Investigation/LOI) Query on PEG | C [auth A] | DI(HYDROXYETHYL)ETHER C4 H10 O3 MTHSVFCYNBDYFN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.29 ?
- R-Value Free: 0.166
- R-Value Work: 0.137
- R-Value Observed: 0.138
- Space Group: P 21 21 21
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 34.589 | ¦Á = 90 |
| b = 46.31 | ¦Â = 90 |
| c = 53.524 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| PDB_EXTRACT | data extraction |
| XDS | data reduction |
| Aimless | data scaling |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2022-11-23 Deposition Author(s): Takebe, K., Chida, T., Suzuki, M., Itagaki, T., Morita, Y., Uzawa, N., Kobayashi, H.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Japan Society for the Promotion of Science (JSPS) | Japan | 20K21676 |
Revision History (Full details and data files)
- Version 1.0: 2022-11-23
Type: Initial release - Version 1.1: 2023-11-29
Changes: Data collection, Refinement description - Version 1.2: 2024-11-06
Changes: Structure summary
