Download MendeleyBacillus subtilis DHFR bound to NADP+ and folate
Smith, N., Horswill, A.R., Wilson, M.A.To be published.
8UVZ
Bacillus subtilis DHFR bound to NADP+ and folate
- PDB DOI: https://doi.org/10.2210/pdb8UVZ/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Bacillus subtilis
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2023-11-05 Released: 2023-11-15
- Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 0.93 ?
- R-Value Free: 0.123
- R-Value Work: 0.110
- R-Value Observed: 0.111
Starting Model: experimental
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This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Dihydrofolate reductase | 168 | Bacillus subtilis | Mutation(s): 0 Gene Names: dfrA EC: 1.5.1.3 |  | |
UniProt | |||||
Find proteins for P11045 (Bacillus subtilis (strain 168)) Explore P11045 Go to UniProtKB: P11045 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P11045 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NAP (Subject of Investigation/LOI) Query on NAP | C [auth A] | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE C21 H28 N7 O17 P3 XJLXINKUBYWONI-NNYOXOHSSA-N |  | ||
| FOL (Subject of Investigation/LOI) Query on FOL | B [auth A] | FOLIC ACID C19 H19 N7 O6 OVBPIULPVIDEAO-LBPRGKRZSA-N |  | ||
| 1PE Query on 1PE | D [auth A] | PENTAETHYLENE GLYCOL C10 H22 O6 JLFNLZLINWHATN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 0.93 ?
- R-Value Free: 0.123
- R-Value Work: 0.110
- R-Value Observed: 0.111
- Space Group: P 61
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 88.45 | ¦Á = 90 |
| b = 88.45 | ¦Â = 90 |
| c = 37.265 | ¦Ă = 120 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| MOSFLM | data reduction |
| Aimless | data scaling |
| CNS | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2023-11-15 Deposition Author(s): Smith, N., Horswill, A.R., Wilson, M.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | R01GM139978 |
Revision History (Full details and data files)
- Version 1.0: 2023-11-15
Type: Initial release - Version 1.1: 2024-11-06
Changes: Structure summary
