9BGQ | pdb_00009bgq

Cryo-EM structure of Trypanosoma cruzi MscS

PDB DOI: https://doi.org/10.2210/pdb9BGQ/pdb
EM Map EMD-44520: EMDB EMDataResource

Classification: TRANSPORT PROTEIN
Organism(s): Trypanosoma cruzi
Expression System: Komagataella pastoris
Mutation(s): No

Deposited: 2024-04-19 Released: 2025-05-07
Deposition Author(s): Zhang, J., Yuan, P.
Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 3.21 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

Starting Model: in silico
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wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Lipid-mediated gating of a miniature mechanosensitive MscS channel from Trypanosoma cruzi

Zhang, J., Bhatt, A., Maksaev, G., Luo, Y., Yuan, P.

To be published.

Macromolecule Content

Total Structure Weight: 131.62 kDa
Atom Count: 7,231
Modelled Residue Count: 959
Deposited Residue Count: 1,218
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Mechanosensitive ion channel MscS domain-containing protein	A, B, C, D, E A, B, C, D, E, F, G	174	Trypanosoma cruzi	Mutation(s): 0 Gene Names: ECC02_000513
UniProt
Find proteins for A0A7J6YIJ5 (Trypanosoma cruzi) Explore A0A7J6YIJ5 Go to UniProtKB: A0A7J6YIJ5
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0A7J6YIJ5
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: ELECTRON MICROSCOPY
Resolution: 3.21 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

EM Software:

Task	Software Package	Version
MODEL REFINEMENT	PHENIX	1.20.1

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2025-05-07

Deposition Author(s):

Funding Organization	Location	Grant Number
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	United States	R01GM143440

Revision History (Full details and data files)

Version 1.0: 2025-05-07
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.