Download MendeleyNanJ sialidase in complex with Neu5,9Ac
Medley, B.J., Boraston, A.To be published.
9C20
The Sialidase NanJ in complex with Neu5,9Ac
- PDB DOI: https://doi.org/10.2210/pdb9C20/pdb
- Classification: HYDROLASE
- Organism(s): Clostridium perfringens ATCC 13124
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2024-05-30 Released: 2024-09-04
- Funding Organization(s): Canadian Glycomics Network (GLYCONET)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.70 ?
- R-Value Free: 0.254
- R-Value Work: 0.184
Starting Model: experimental
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This is version 1.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| exo-alpha-sialidase | 451 | Clostridium perfringens ATCC 13124 | Mutation(s): 5 Gene Names: nanJ, CPF_0532 EC: 3.2.1.18 |  | |
UniProt | |||||
Find proteins for A0A0H2YT71 (Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A)) Explore A0A0H2YT71 Go to UniProtKB: A0A0H2YT71 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A0H2YT71 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| 5N6 (Subject of Investigation/LOI) Query on 5N6 | C [auth A] | 9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonic acid C13 H21 N O10 NYWZBRWKDRMPAS-CTAKWSDESA-N |  | ||
| EDO Query on EDO | B [auth A] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.70 ?
- R-Value Free: 0.254
- R-Value Work: 0.184
- Space Group: P 64 2 2
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 171.037 | ¦Á = 90 |
| b = 171.037 | ¦Â = 90 |
| c = 93.296 | ¦Ă = 120 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-09-04 Deposition Author(s): Medley, B.J., Low, K.E., Garber, J.M., Gray, T.E., Leeann, L.L., Fordwour, O.B., Inglis, G.D., Boons, G.J., Zandberg, W.F., Abbott, W., Boraston, A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Canadian Glycomics Network (GLYCONET) | Canada | -- |
Revision History (Full details and data files)
- Version 1.0: 2024-09-04
Type: Initial release
