Download MendeleyMolecular basis of mRNA delivery to the bacterial ribosome
Webster, M.W., Chauvier, A., Rahil, H., Graziadei, A., Miropolskaya, N., Charles, K., Takacs, M., Saintandre, C., Rappsilber, J., Walter, N.G., Weixlbaumer, A.(2024) Science
9GUR
30S mRNA delivery complex TEC resolved (TEC only)
- PDB DOI: https://doi.org/10.2210/pdb9GUR/pdb
- EM Map EMD-51617: EMDB EMDataResource
- Classification: RIBOSOME
- Organism(s): Escherichia coli K-12, synthetic construct
- Expression System: Escherichia coli K-12
- Mutation(s): No
- Deposited: 2024-09-20 Released: 2024-11-20
- Funding Organization(s): European Research Council (ERC), Agence Nationale de la Recherche (ANR), European Molecular Biology Organization (EMBO), National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Wellcome Trust
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 4.20 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit alpha | A [auth 1] | 229 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: rpoA, pez, phs, sez, b3295, JW3257 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for P0A7Z4 (Escherichia coli (strain K12)) Explore P0A7Z4 Go to UniProtKB: P0A7Z4 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0A7Z4 | ||||
Sequence AnnotationsExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit omega | B [auth 5] | 71 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: rpoZ, b3649, JW3624 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for P0A800 (Escherichia coli (strain K12)) Explore P0A800 Go to UniProtKB: P0A800 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0A800 | ||||
Sequence AnnotationsExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 6 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit alpha | F [auth 2] | 230 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: rpoA, pez, phs, sez, b3295, JW3257 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for P0A7Z4 (Escherichia coli (strain K12)) Explore P0A7Z4 Go to UniProtKB: P0A7Z4 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0A7Z4 | ||||
Sequence AnnotationsExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 7 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit beta | G [auth 3] | 1,342 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for P0A8V2 (Escherichia coli (strain K12)) Explore P0A8V2 Go to UniProtKB: P0A8V2 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0A8V2 | ||||
Sequence AnnotationsExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 8 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA-directed RNA polymerase subunit beta' | H [auth 4] | 1,359 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: rpoC, tabB, b3988, JW3951 EC: 2.7.7.6 |  |
UniProt | |||||
Find proteins for P0A8T7 (Escherichia coli (strain K12)) Explore P0A8T7 Go to UniProtKB: P0A8T7 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0A8T7 | ||||
Sequence AnnotationsExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 9 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Transcription termination/antitermination protein NusG | I [auth Z] | 113 | Escherichia coli K-12 | Mutation(s): 0 Gene Names: nusG, b3982, JW3945 |  |
UniProt | |||||
Find proteins for P0AFG0 (Escherichia coli (strain K12)) Explore P0AFG0 Go to UniProtKB: P0AFG0 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0AFG0 | ||||
Sequence AnnotationsExpand | |||||
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Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| Non-Template DNA strand | C [auth 6] | 30 | synthetic construct |  | |
Sequence AnnotationsExpand | |||||
| |||||
Find similar nucleic acids by:
(by identity cutoff) | 3D Structure
Entity ID: 4 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| Template DNA strand | D [auth 7] | 30 | synthetic construct |  | |
Sequence AnnotationsExpand | |||||
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Find similar nucleic acids by: Sequence | 3D Structure
Entity ID: 5 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| mRNA | E [auth X] | 16 | synthetic construct |  | |
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ZN Query on ZN | K [auth 4], L [auth 4] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | ||
| MG Query on MG | J [auth 4] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 4.20 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
Entry History & Funding Information
Deposition Data
- Released Date: 2024-11-20 Deposition Author(s): Rahil, H., Weixlbaumer, A., Webster, M.W.
| Funding Organization | Location | Grant Number |
|---|---|---|
| European Research Council (ERC) | European Union | 679734 |
| Agence Nationale de la Recherche (ANR) | France | ANR-10-LABX-0030-INRT |
| Agence Nationale de la Recherche (ANR) | France | ANR-10-IDEX-0002-02 |
| European Molecular Biology Organization (EMBO) | European Union | -- |
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | R35GM131922 |
| Wellcome Trust | United Kingdom | 203149 |
Revision History (Full details and data files)
- Version 1.0: 2024-11-20
Type: Initial release
