9JOM

STRUCTURE OF ENDO-1,3-FUCANASE FUN168E FROM GH168 FAMILY

PDB DOI: https://doi.org/10.2210/pdb9JOM/pdb

Classification: HYDROLASE
Organism(s): Wenyingzhuangia fucanilytica
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2024-09-25 Released: 2024-11-27
Deposition Author(s): Chen, G.N., Chang, Y.G.
Funding Organization(s): National Natural Science Foundation of China (NSFC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.04 ?
R-Value Free: 0.234
R-Value Work: 0.177
R-Value Observed: 0.181

Starting Model: in silico
View more details

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Structure of endo-1.3-fucanase (Fun168E) at 2.04 Angstroms resulution.

Chen, G.N., Chang, Y.G.

To be published.

Macromolecule Content

Total Structure Weight: 45.57 kDa
Atom Count: 3,286
Modelled Residue Count: 354
Deposited Residue Count: 394
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Glycoside-hydrolase family GH114 TIM-barrel domain-containing protein	A	394	Wenyingzhuangia fucanilytica	Mutation(s): 0 Gene Names: AXE80_08855
UniProt
Find proteins for A0A1B1Y6K9 (Wenyingzhuangia fucanilytica) Explore A0A1B1Y6K9 Go to UniProtKB: A0A1B1Y6K9
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0A1B1Y6K9
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.04 ?
R-Value Free: 0.234
R-Value Work: 0.177
R-Value Observed: 0.181
Space Group: P 3₂ 2 1

Unit Cell:

Length ( ? )	Angle ( ? )
a = 83.135	α = 90
b = 83.135	β = 90
c = 100.43	γ = 120

Software Package:

Software Name	Purpose
PHENIX	refinement
Aimless	data scaling
XDS	data reduction
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2024-11-27

Deposition Author(s):

Funding Organization	Location	Grant Number
National Natural Science Foundation of China (NSFC)	China	U22A20542

Revision History (Full details and data files)

Version 1.0: 2024-11-27
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.