Download MendeleyStructural and Functional Analysis of SAM-Dependent N-Methyltransferases Involved in Ovoselenol and Ovothiol Biosynthesis
Ireland, K.A., Kayrouz, C.M., Abbott, M.L., Seyedsayamdost, M.R., Davis, K.M.(2024) Structure
9BXH
OvsM from Marinimicrobium koreense, a SAM-dependent N-methyltransferase involved in ovoselenol biosynthesis
- PDB DOI: https://doi.org/10.2210/pdb9BXH/pdb
- Classification: TRANSFERASE
- Organism(s): Marinimicrobium koreense
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2024-05-22 Released: 2025-01-08
- Funding Organization(s): National Science Foundation (NSF, United States), National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.48 ?
- R-Value Free: 0.196
- R-Value Work: 0.176
- R-Value Observed: 0.177
Starting Model: in silico
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wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| 5-selenohistidine N-methyltransferase OvsM | 267 | Marinimicrobium koreense | Mutation(s): 0 Gene Names: EDC38_1804 |  | |
UniProt | |||||
Find proteins for A0A3N1P0S2 (Marinimicrobium koreense) Explore A0A3N1P0S2 Go to UniProtKB: A0A3N1P0S2 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A3N1P0S2 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| EDO Query on EDO | B [auth A], C [auth A], D [auth A] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.48 ?
- R-Value Free: 0.196
- R-Value Work: 0.176
- R-Value Observed: 0.177
- Space Group: C 2 2 21
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 83.838 | ¦Á = 90 |
| b = 90.058 | ¦Â = 90 |
| c = 80.321 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| XDS | data reduction |
| XDS | data scaling |
| PHASER | phasing |
| Coot | model building |
| PHENIX | refinement |
Entry History & Funding Information
Deposition Data
- Released Date: 2025-01-08 Deposition Author(s): Ireland, K.A., Davis, K.M.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Science Foundation (NSF, United States) | United States | 1937971 |
| National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM147557 |
Revision History (Full details and data files)
- Version 1.0: 2025-01-08
Type: Initial release
