9HJ8 | pdb_00009hj8

TRPML1 in complex with compound 5

PDB DOI: https://doi.org/10.2210/pdb9HJ8/pdb
EM Map EMD-52211: EMDB EMDataResource

Classification: MEMBRANE PROTEIN
Organism(s): Homo sapiens
Expression System: Homo sapiens
Mutation(s): No

Deposited: 2024-11-28 Released: 2025-06-04
Deposition Author(s): Reeks, J., Mahajan, P., Clark, M., Cowan, S.R., Di Daniel, E., Earl, C.P., Fisher, S., Holvey, R.S., Jackson, S.M., Lloyd-Evans, E., Morgillo, C.M., Mortenson, P.N., O'Reilly, M., Richardson, C.J., Schopf, P., Tams, D.M., Waller-Evans, H., Ward, S.E., Whibley, S., Williams, P.A., Johnson, C.N.
Funding Organization(s): Not funded

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 2.10 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

Currently 9HJ8 does not have a validation slider image.

This is version 1.0 of the entry. See complete history.

Literature

Enabling High Throughput Electron Cryo-microscopy for Structure-based Design

Reeks, J., Mahajan, P., Clark, M., Cowan, S.R., Di Daniel, E., Earl, C.P., Fisher, S., Holvey, R.S., Jackson, S.M., Lloyd-Evans, E., Morgillo, C.M., Mortenson, P.N., O'Reilly, M., Richardson, C.J., Schopf, P., Tams, D.M., Waller-Evans, H., Ward, S.E., Whibley, S., Williams, P.A., Johnson, C.N.

To be published.

Macromolecule Content

Total Structure Weight: 292.9 kDa
Atom Count: 15,884
Modeled Residue Count: 1,836
Deposited Residue Count: 2,512
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Mucolipin-1	A, B, C, D	628	Homo sapiens	Mutation(s): 0 Gene Names: MCOLN1, ML4, TRPML1, MSTP080
UniProt & NIH Common Fund Data Resources
Find proteins for Q9GZU1 (Homo sapiens) Explore Q9GZU1 Go to UniProtKB: Q9GZU1
PHAROS: Q9GZU1 GTEx: ENSG00000090674
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9GZU1
Glycosylation
Glycosylation Sites: 1	Glycosylation Focus chain A Focus chain B Focus chain C Focus chain D	Go to GlyGen: Q9GZU1-1
Sequence Annotations Expand
Reference Sequence

Oligosaccharides

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Entity ID: 2
Molecule	Chains	Length	2D Diagram	Glycosylation	3D Interactions
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	E, F, G, H	2		N-Glycosylation	Interactions Focus chain E Focus chain F Focus chain G Focus chain H Interactions & Density Focus chain E Focus chain F Focus chain G Focus chain H

Small Molecules

Ligands 4 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
PC1 Query on PC1 Download Ideal Coordinates CCD File SDF format, chain K [auth A] SDF format, chain U [auth C] SDF format, chain Z [auth D] SDF format, chain P [auth B] MOL2 format, chain K [auth A] MOL2 format, chain U [auth C] MOL2 format, chain Z [auth D] MOL2 format, chain P [auth B] mmCIF format, chain K [auth A] mmCIF format, chain U [auth C] mmCIF format, chain Z [auth D] mmCIF format, chain P [auth B]	K [auth A], P [auth B], U [auth C], Z [auth D]	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE C₄₄ H₈₈ N O₈ P NRJAVPSFFCBXDT-HUESYALOSA-N		Interactions Focus chain K [auth A] Focus chain P [auth B] Focus chain U [auth C] Focus chain Z [auth D] Interactions & Density Focus chain K [auth A] Focus chain P [auth B] Focus chain U [auth C] Focus chain Z [auth D]
EUJ Query on EUJ Download Ideal Coordinates CCD File SDF format, chain I [auth A] SDF format, chain S [auth C] SDF format, chain X [auth D] SDF format, chain N [auth B] MOL2 format, chain I [auth A] MOL2 format, chain S [auth C] MOL2 format, chain X [auth D] MOL2 format, chain N [auth B] mmCIF format, chain I [auth A] mmCIF format, chain S [auth C] mmCIF format, chain X [auth D] mmCIF format, chain N [auth B]	I [auth A], N [auth B], S [auth C], X [auth D]	(2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dioctanoate C₂₅ H₄₉ O₁₉ P₃ QXHVLVSULWMTCV-DICZBTHZSA-N		Interactions Focus chain I [auth A] Focus chain N [auth B] Focus chain S [auth C] Focus chain X [auth D] Interactions & Density Focus chain I [auth A] Focus chain N [auth B] Focus chain S [auth C] Focus chain X [auth D]
A1IVD (Subject of Investigation/LOI) Query on A1IVD Download Ideal Coordinates CCD File SDF format, chain J [auth A] SDF format, chain O [auth B] SDF format, chain Y [auth D] SDF format, chain T [auth C] MOL2 format, chain J [auth A] MOL2 format, chain O [auth B] MOL2 format, chain Y [auth D] MOL2 format, chain T [auth C] mmCIF format, chain J [auth A] mmCIF format, chain O [auth B] mmCIF format, chain Y [auth D] mmCIF format, chain T [auth C]	J [auth A], O [auth B], T [auth C], Y [auth D]	N,N-dimethyl-4-[1'-[2,2,2-tris(fluoranyl)ethyl]spiro[2H-indole-3,4'-piperidine]-1-yl]sulfonyl-benzenesulfonamide C₂₂ H₂₆ F₃ N₃ O₄ S₂ OUXVDEHHRJIYFH-UHFFFAOYSA-N		Interactions Focus chain J [auth A] Focus chain O [auth B] Focus chain T [auth C] Focus chain Y [auth D] Interactions & Density Focus chain J [auth A] Focus chain O [auth B] Focus chain T [auth C] Focus chain Y [auth D]
OCT Query on OCT Download Ideal Coordinates CCD File SDF format, chain AA [auth D] SDF format, chain BA [auth D] SDF format, chain L [auth A] SDF format, chain M [auth A] SDF format, chain Q [auth B] SDF format, chain R [auth B] SDF format, chain W [auth C] SDF format, chain V [auth C] MOL2 format, chain AA [auth D] MOL2 format, chain BA [auth D] MOL2 format, chain L [auth A] MOL2 format, chain M [auth A] MOL2 format, chain Q [auth B] MOL2 format, chain R [auth B] MOL2 format, chain W [auth C] MOL2 format, chain V [auth C] mmCIF format, chain AA [auth D] mmCIF format, chain BA [auth D] mmCIF format, chain L [auth A] mmCIF format, chain M [auth A] mmCIF format, chain Q [auth B] mmCIF format, chain R [auth B] mmCIF format, chain W [auth C] mmCIF format, chain V [auth C]	AA [auth D] BA [auth D] L [auth A] M [auth A] Q [auth B] AA [auth D], BA [auth D], L [auth A], M [auth A], Q [auth B], R [auth B], V [auth C], W [auth C]	N-OCTANE C₈ H₁₈ TVMXDCGIABBOFY-UHFFFAOYSA-N		Interactions Focus chain AA [auth D] Focus chain BA [auth D] Focus chain L [auth A] Focus chain M [auth A] Focus chain Q [auth B] Focus chain R [auth B] Focus chain V [auth C] Focus chain W [auth C] Interactions & Density Focus chain AA [auth D] Focus chain BA [auth D] Focus chain L [auth A] Focus chain M [auth A] Focus chain Q [auth B] Focus chain R [auth B] Focus chain V [auth C] Focus chain W [auth C]